张雷;贾玥;王继源;陶建敏;

• 1:南京农业大学园艺学院

摘要(Abstract)：

【目的】探讨普通单层白色纸袋和双层黑色纸袋对葡萄果实品质、花色苷组分及合成相关基因表达的影响。【方法】以‘美人指’葡萄为试材,采用HPLC方法研究花色苷含量与组成,采用荧光PCR技术研究花色苷合成相关基因表达的影响。【结果】套袋对‘美人指’葡萄果实品质影响不显著;在‘美人指’葡萄果皮中检测到了12种花色苷,黑袋处理显著提高了花色苷含量;黑袋处理下PAL、4CL、CHS1、CHS3、CHI1、F3’5’H、DFR、LDOX、OMT、3GT、5GT、MYB5a、Vlmyb A 1-1等花色苷合成相关基因上调表达,提高了这些基因的转录水平。【结论】双层黑色纸袋处理显著促进了花色苷的合成及相关基因的表达,从而使葡萄果皮更好的着色。

关键词(KeyWords)： ‘美人指’葡萄;套袋;花色苷;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系(CARS-30);; 江苏省科技厅(BE2012453);; 江苏省农业自主创新基金[CX(12)3005]

作者(Author): 张雷;贾玥;王继源;陶建敏;

Email:

DOI: 10.13925/j.cnki.gsxb.20140135

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