张倩;贝峰;孙欣;王明林;辛力;

• 1:山东省果树研究所
• 2:山东农业大学食品科学与工程学院
• 3:泰安出入境检验检疫局

摘要(Abstract)：

【目的】寻求建立甜樱桃果实中赤霉酸的定性定量检测方法,为研究赤霉素在大棚甜樱桃的使用安全性上提供方法参考。【方法】样品经乙腈提取,液-液分配法进行净化,采用高效液相色谱-串联质谱(HPLC-MS/MS)法在SRM模式下对目标物质进行定性和定量检测。【结果】方法的最低检出限(S/N=3)为1.21μg·kg~(-1),定量限(S/N=10)为4.03μg·kg~(-1),在5.0~100.0μg·L~(-1)内呈现良好的线性关系,相关系数r2为0.999 3,添加浓度为0.001、0.002、0.01 mg·kg~(-1)时赤霉素加标回收率分别为89.69%,91.84%和95.37%(n=6),相对标准偏差分别为7.46%,6.21%和4.85%,方法的不确定度为0.13μg·kg~(-1),5份大棚樱桃样品中GA_3含量在21.3~71.1μg·kg~(-1)。【结论】本方法简单快速,灵敏度高,定量准确,可用于樱桃果实中的赤霉素残留量测定。

关键词(KeyWords)： 甜樱桃;赤霉酸;高效液相色谱-串联质谱;定性定量检测

Abstract:

Keywords:

基金项目(Foundation): 山东省现代农业产业技术体系水果创新团队建设(SDAIT-06-13);; 山东省农业重大应用技术创新项目——樱桃采后商品化处理设备研制与试验示范;; 泰安市科技计划重大专项(201340629)

作者(Author): 张倩;贝峰;孙欣;王明林;辛力;

Email:

DOI: 10.13925/j.cnki.gsxb.20160298

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