邢丽敏;槐心体;张新忠;王忆;韩振海;

• 1:中国农业大学农学与生物技术学院园艺植物研究所

摘要(Abstract)：

【目的】为了解苹果实生砧木资源重要砧木性状的种间、居群间和株系间差异及株系内性状分离,【方法】搜集了7个种21份苹果资源的种子,建立实生群体,播种当年调查实生苗株高、干粗、叶形指数和分枝数等形态性状,当年秋芽接富士和金冠品种,第2年调查实生苗越冬抽条率、耐盐性、嫁接芽萌芽率、抽梢率和成苗率。通过接种鉴定评价腐烂病和轮纹病的抗病性。【结果】形态性状种间差异显著,平邑甜茶形态一致性好。1 a生实生苗耐越冬抽条能力种间差异大。栽培品种实生苗耐抽条能力差,且易感苹果轮纹病。八棱海棠和平顶海棠耐盐碱性较强,山定子耐盐碱性差,且居群间差异极显著。腐烂病和轮纹病的抗病性均存在种间、居群间或株系间差异及严重的株系内性状分离。平邑甜茶和小金海棠的嫁接芽抽梢率和秋季成苗率均低。【结论】多数性状不仅种间、居群间或株系间差异显著,株系内性状也严重分离。选育、应用苹果无性系砧木是迫切需求。

关键词(KeyWords)： 苹果;实生砧木;种质资源;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业(农业)科研专项(201203075);; 国家苹果产业技术体系;; 国家现代苹果产业技术体系;; 农业部园艺作物营养与生理重点实验室;; 农业科研杰出人才及其创新团队项目

作者(Authors): 邢丽敏;槐心体;张新忠;王忆;韩振海;

DOI: 10.13925/j.cnki.gsxb.2013.04.010

参考文献(References)：

• [1]LI Yu-nong.Researches of germplasm resources of Malus Mill.[M].Beijing:China Agriculture Press,2001.李育农.苹果属植物种质资源研究[M].北京:中国农业出版社,2001.
• [2]HOKANSON S C,LAMBOY W F,SZEWC-MCFADDEN A K,MCFERSON G R.Microsatellite(SSR)variation in collection ofMalus(apple)species and hybrids[J].Euphytica,2001,118:281-294.
• [3]LIU Zun-chun,MIAO Wei-dong,LIU Da-liang,CHEN Xue-sen.Construction and evaluation of the segregation population in Malussieversii[J].Journal of Fruit Science,2012,29(5):722-728.刘遵春,苗卫东,刘大亮,陈学森.新疆野苹果分离群体的构建和评价[J].果树学报,2012,29(5):722-728.
• [4]BENSON L L,LAMBOY W F,ZIMMERMAN R H.Molecular iden-tification of Malus hupehensis(tea crabapple)accessions using Sim-ple Sequence Repeats[J].HortSci,2001,36(5):961-966.
• [5]YAN Guo-rong,LONG Hong,SONG Wen-qin,CHEN Rui-yang.Genetic polymorphism of Malus sieversii populations in Xinjiang,China[J].Genetic Resources and Crop Evolution,2008,55:171-181.
• [6]ZHANG Chun-yu,CHEN Xue-sen,HE Tian-ming,LIU Xiao-li,FENG Tao,YUAN Zhao-he.Genetic structure of Malus sieversiipopulation from Xinjiang,China,revealed by SSR Markers[J].Journalof Genetics and Genomics,2007,34(10):947-955.
• [7]RICHARDS C M,VOLK G M,REILLEY A A,HENK A D,LOCK-WOOD D R,REEVES P A,FORSLINE P L.Genetic diversity andpopulation structure in Malus sieversii,a wild progenitor species ofdomesticated apple[J].Tree Genetics&Genomes,2009,5:339-347.
• [8]QIN Wei,SHA Hong,LIU Li-qiang,LIAO Kang,GENG Wen-juan,WANG Yun.SSR analysis for genetic diversity of Malus sieversiifrom Xinjiang,China[J].Journal of Fruit Science,2012,29(2):161-165.秦伟,沙红,刘立强,廖康,耿文娟,王云.新疆野苹果资源遗传多样性SSR分析[J].果树学报,2012,29(2):161-165.
• [9]ZHANG Jing-guo,HU Hong-ju,XU Yu-hai,LUO Zheng-rong.Germplasm identification and genetic relationship of some Malus hu-pehensis(Pamp.)Rehd.accessions[J].Journal of Huazhong Agri-cultural University,2009,28(6):736-740.张靖国,胡红菊,徐育海,罗正荣.部分湖北海棠种质资源的鉴定及亲缘关系分析[J].华中农业大学学报,2009,28(6):736-740.
• [10]CHEN Xi,TANG Geng-guo,ZHENG Yu-hong,WANG Lei-hong.RAPD analysis of genetic diversity of Malus baccata(L.)Borkh[J].Acta Bot Boreal-Occident Sin,2008,28(10):1954-1959.陈曦,汤庚国,郑玉红,王雷宏.苹果属山荆子遗传多样性的RAPD分析[J].西北植物学报,2008,28(10):1954-1959.
• [11]SHI Sheng-you,CHENG Ming-hao,LIANG Guo-lu,GUO Qi-gao,LI Xiao-lin,ZHOU Zhi-qin.Genetic diversity of Malus toringoides(Rehd.)Hughes based on AFLP[J].Acta Horticulturae Sinica,2006,33(2):381-384.石胜友,成明昊,梁国鲁,郭启高,李晓林,周志钦.变叶海棠遗传多样性的AFLP分析[J].园艺学报,2006,33(2):381-384.
• [12]LIU Hai-tao,LI Cai-li,ZHANG Yu-jin,LI Chun-min,ZHAOYong-bo,CHEN Dong-mei,WANG Yi,ZHANG Xin-zhong,HANZhen-hai.Inheritance and molecular marker of resistance to botcanker in Malus domestica[J].Agricultural Sciences in China,2011,10(2):175-184.
• [13]LIU Hai-tao,ZHANG Yu-jing,LI Chun-min,WANG Yi,LIU Guo-jian,CHANG Rui-feng,ZHAO Yong-bo,XU Xue-feng,HANZhen-hai,ZHANG Xin-zhong.Evaluation of the resistance of Malusgermplasm to bot canker caused by botryosphaeria dothidea[J].JPhytopathol,2011(159):511-515.
• [14]ZANG Rui,HUANG Li-li,KANG Zhen-sheng,WANG Xu-li.Bio-logical characteristics and pathogenicity of different isolates of Cy-tospora spp.isolated from apple trees in Shannxi province(in Chi-nese)[J].Acta Phytopa thologica Sinica,2007,37(4):343-351.臧睿,黄丽丽,康振生,王旭丽.陕西苹果腐烂病(Cytopora spp.)不同分离株的生物学特性与致病性研究[J].植物病理学报,2007,37(4):343-351.
• [15]LIU Xin-ying,LSong,WANG Yi,WANG Kun,LI Tian-hong,HAN Zhen-hai,ZHANG Xin-zhong.Evaluation of resistance ofMalus germplasms to apple canker(Valsa ceratosperma)[J].Journalof Fruit Science,2011,28(5):843-848.刘欣颖,吕松,王忆,王昆,李天红,韩振海,张新忠.苹果种质资源对苹果腐烂病抗性评价[J].果树学报,2011,28(5):843-848.
• [16]LI Shao-hua,LUO Zheng-rong,LIU Guo-jie,PENG Shu-ang.Intro-duction to pomiculture[M].Beijing:Higher Education Press,1999:24-27.李绍华,罗正荣,刘国杰,彭抒昂.果树栽培概论[M].北京:高等教育出版社,1999:24-27.
• [17]Changli Institute of Fruit Science.Apple of Hebei[M].AgriculturalPublishing House,1985.河北昌黎果树研究所编.河北省苹果志[M].北京:农业出版社,1985.
• [18]SHU Huai-rui.Pomology[M].Beijing:China Agriculture Press,1999:414-416.束怀瑞.果树学[M].北京:中国农业出版社,1999:414-416.
• [19]SU Shi-rong.The harmful effects of using apple varieties as rootstock[J].China Fruits,2009(6):73.苏世荣.苹果共砧苗的危害[J].中国果树,2009(6):73.
• [20]WANG Kun,LIU Feng-zhi,ZHAO Jin-chun,GONG Xin,GAOYuan,LIU Li-jun.Apple Germplasm Resources section of pheno-typic diversity[J].China Fruits,2008(5):20-25.王昆,刘凤之,赵进春,龚欣,高源,刘立军.苹果种质资源部分表型多样性研究[J].中国果树,2008(5):20-25.
• [21]MA Hong-ju,HE Ping,DENG Hong-ping,CHEN Jian-min,ZOUXin-hui.Analyses of morphological differentiation within six popu-lations of Malus hupehensis(Rosaceae)[J].Journal of SouthwestChina Normal University:Natural Science Edition,2002,27(4):261-264.马洪菊,何平,邓洪平,陈建民,邹新慧.湖北海棠不同居群形态变异的数量分析[J].西南师范大学学报:自然科学版,2002,27(4):261-264.
• [22]LIU Jing,ZHOU Qing-he,SUN Hai-wei,QIU Zhi-lin,WANG Yu-wen,SUN Zhong-xu.Study on the phenotype biodiversity of XinjiangWild Apples(Malus sieversii)[J].Journal of Fruit Science,2004,21(4):285-288.刘静,周庆和,孙海伟,邱治霖,王玉文,孙仲序.新疆野生苹果表型多样性研究[J].果树学报,2004,21(4):285-288.
• [23]WANG Lei-hong,TANG Geng-guo.Phenotypic variations of exsic-cate-specimen of Malus baccata(L.)Borkh[J].Acta Bot Boreal-Oc-cident Sin,2007,27(8):1690-1694.王雷宏,汤庚国.山荆子腊叶标本表型性状变异分析[J].西北植物学报,2007,27(8):1690-1694.
• [24]DENG Hong-ping,CHENG Ming-hao,CHEN Ya-fei,HUANG Lin.A study on the relation between the variant types of MalusToringoides and Malus Kansuensis[J].Journal of Southwest ChinaNormal University:Natural Science Edition,2002,27(1):78-83.邓洪平,成明昊,陈亚飞,黄琳.变叶海棠变异类型的形态多样性分化与陇东海棠关系的研究[J].西南师范大学学报:自然科学版,2002,27(1):78-83.
• [25]LIU Han-zhong,REN Qing-mian,LIU Li-jun.Research on the re-sistant of apple canker identification traits[J].Journal of Fruit Science,1990,7(2):65-70.刘捍中,任庆棉,刘立军.苹果属种质资源抗腐烂病性状鉴定研究[J].果树科学,1990,7(2):65-70.
• [26]ZHANG Yu-jing,WANG Kun,WANG Yi,HAN Zhen-hai,GAOYuan,XU Xue-feng,ZHANG Xin-zhong.Evaluation of resistance tofruit ring rot for apple germplasms[J].Acta Horticulturae Sinica,2010,37(4):539-546.张玉经,王昆,王忆,韩振海,高源,许雪峰,张新忠.苹果种质资源果实轮纹病抗性评价[J].园艺学报,2010,37(4):539-546.
• [27]DONG Shao-zheng,YU Hong.Malus plants and apomixes[J].Journalof Fruit Science,1987,4(4):27-29.董绍珍,俞宏.苹果属植物与无融合生殖[J].果树科学,1987,4(4):27-29.
• [28]CHENG Ming-hao,YANG Xiao-hong,ZENG Wei-guang.A pre-liminary report on investigation and study of M.xiaojinensis-an ap-ple stock resource[J].J Southwest University,1984,6(3):38-43.成明昊,杨晓红,曾维光.苹果砧木资源-小金海棠的调查研究初报[J].西南农学院学报,1984,6(3):38-43.
• [29]WANG Lei,HAN De-guo,GAO Chao,WANG Yi,ZHANG Xin-zhong,XU Xue-feng,HAN Zhen-Hai.Paternity and ploidy segrega-tion of progenies derived from tetraploid Malus xiaojinensis[J].TreeGenetics&Genomes,2012,8:1469-1476.
• [30]CHENG Ming-hao,LI Xiao-lin,ZHANH Yun-gui.Malus xiaoji-nensi-A promising stock for apple tree[J].Journal of Southwest Agri-cultural University,2000,22(5):383-386.成明昊,李晓林,张云贵.苹果优良砧木资源-小金海棠[J].西南农业大学学报,2000,22(5):383-386.