杨锋,伊凯,吴雅琴,刘丹丹,刘志,赵玲玲

• 1:辽宁省果树科学研究所
• 2:河北省农林科学院昌黎果树研究所
• 3:山东农业大学园艺科学与工程学院
• 4:烟台市农林科学院果树科学研究所

摘要(Abstract)：

【目的】为探明平邑甜茶的无融合生殖遗传与发育机制,【方法】以平邑甜茶与B9、M27、M9为亲本杂交,并筛选杂种实生苗;用去雄套袋法鉴定杂种的无融合生殖率,用流式细胞仪鉴定杂种的染色体倍性。【结果】获得种子3101粒,培育实生苗2 388株,从中筛选出杂种实生苗73株,其中58株开始开花结果。经无融合生殖率鉴定,有25株为84.00%~95.06%,8株为10.00%~48.00%,25株为0~5.00%;倍性鉴定表明,10株四倍体皆为无融合生殖型,4株二倍体皆为有性生殖型,三倍体植株中无融合生殖型加上中间型与有性生殖型数量比接近1∶1。【结论】验证了平邑甜茶的无融合生殖以显性单基因质量性状为主,也有数量性状效应;初步认为平邑甜茶无融合生殖核基因型为"Aaa"("A"代表无融合生殖基因,显性;"a"代表有性生殖基因,隐性);平邑甜茶胚囊多数情况下由未减数的2n=3x配子直接发育成与母株基因相同的胚,部分2n配子与雄配子结合,形成2n+n=4x型后代,少量胚囊经减数分裂产生n=x、n=2x两种配子,无融合生殖基因随n=2x配子分离。

关键词(KeyWords)： 平邑甜茶;无融合生殖;核基因型;雌配子发育

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(nycytx-08-01-05)

作者(Author): 杨锋,伊凯,吴雅琴,刘丹丹,刘志,赵玲玲

DOI: 10.13925/j.cnki.gsxb.2012.04.011

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