陈世昌,侯殿明,吴文祥,孙文英,邱立友

• 1:河南农业职业学院
• 2:中国科学院地理科学与资源研究所
• 3:河南农业大学生命科学学院

摘要(Abstract)：

【目的】为探明套种平菇对梨园土壤生物活性及梨果实品质的影响,【方法】在7 a生梨树行间覆土套种平菇,将出菇后的菇渣旋耕还田,以不套种平菇为对照,测定分析梨园0～60 cm不同土层中细菌、放线菌、真菌数量,微生物量碳、量氮,脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性以及成熟梨果实品质。【结果】结果表明,套种平菇显著提高0～40 cm土层3大类群土壤微生物数量、生物量及脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性,并随套种年限的增加趋于增加。连续3 a套种平菇处理的细菌、放线菌、真菌数量较对照提高47.4%、32.0%、125.1%;微生物量碳、量氮比对照提高25.6%、28.1%;土壤脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性提高42.3%、47.2%、29.5%、11.6%。土壤微生物数量、生物量、脲酶、蔗糖酶和磷酸酶活性随土层加深均有所降低,而过氧化氢酶活性则呈升高趋势。套种平菇后能不同程度改善果实品质,显著提高了梨果单果质量、硬度、可溶性固形物及可溶性糖含量,降低了可滴定酸含量。【结论】套种平菇对提高梨园0～40 cm土层土壤肥力、改善梨品质有显著效果。

关键词(KeyWords)： 梨园;平菇;土壤微生物;生物量;酶活性;品质

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41172154);; 河南省重点科技攻关计划项目(082102110004)

作者(Author): 陈世昌,侯殿明,吴文祥,孙文英,邱立友

DOI: 10.13925/j.cnki.gsxb.2012.04.018

参考文献(References)：

• [1]DORAN J,SARRANTONIO M,LIEBIG M A.Soil health and sus-tainability[J].Advances in Agronomy,1996,56:1-54.
• [2]ACOSTA-MARTINEZ V,BUROW C,ZOBECK T M,ALLEN V G.Soil microbial community and function in alternative systems to con-tinuous cotton[J].Soil Science Society of America Journal,2010,74(4):1181-1192.
• [3]LIU En-ke,ZHAO Bing-qiang,LI Xiu-ying,JIANG Rui-bo,LIYan-ting,HWAT Bing So.Biological properties and enzymatic ac-tivity of arable soils affected by long-term different fertilization sys-tems[J].Journal of Plant Ecology,2008,32(1):176-182.刘恩科,赵秉强,李秀英,姜瑞波,李燕婷,HWAT B S.长期施肥对土壤微生物量及土壤酶活性的影响[J].植物生态学报,2008,32(1):176-182.
• [4]QIU Li-ping,LIU Jun,WANG Yi-quan,SUN Hui-ming,HE Wen-xiang.Research on relationship between soil enzyme activities andsoil fertility[J].Plant Nutrition and Fertilizer Science,2004,10(3):277-280.邱莉萍,刘军,王益权,孙慧敏,和文祥.土壤酶活性与土壤肥力的关系研究[J].植物营养与肥料学报,2004,10(3):277-280.
• [5]LI Hui-ke,ZHANG Guang-jun,ZHAO Zheng-yang,LI Kai-rong.Effects of interplanting of herbage on soil nutrient of non-irrigatedapple orchards in the Loess Plateau[J].Acta Horticulturae Sinica,2007,34(2):477-480.李会科,张广军,赵政阳,李凯荣.黄土高原旱地苹果园生草对土壤养分的影响[J].园艺学报,2007,34(2):477-480.
• [6]DENG Feng-chan,AN Gui-yang,YU Jun-yi,DU Zhi-hui.Researchon growing grass in apple orchard in Weibei upland[J].Journal ofFruit Science,2003,20(6):506-508.邓丰产,安贵阳,郁俊谊,杜志辉.渭北旱塬苹果园的生草效应[J].果树学报,2003,20(6):506-508.
• [7]WU Hong-ying,KONG Yun,YAO Yun-cong,BI Ning-ning,YUANLi-ping,FU Zhan-guo.Effects of intercropping aromatic plants onsoil microbial quantity and soil nutrients in pear orchard[J].ScientiaAgricultura Sinica,2010,43(1):140-150.吴红英,孔云,姚允聪,毕宁宁,亓丽萍,付占国.间作芳香植物对沙地梨园土壤微生物数量与土壤养分的影响[J].中国农业科学,2010,43(1):140-150.
• [8]ZHOU Qi-ji.Agricultural Microbiology[M].(2rd ed).Beijing:ChinaAgriculture Press,2010.周奇迹.农业微生物[M].(第二版).北京:中国农业出版社2010.
• [9]JOERGENSEN R G.The fumigation-extraction method to estimatesoil microbial biomass:Calibration of the KEC value[J].Soil Biology&Biochemistry,1996,28(1):25-31.
• [10]VANCE E D,BROOKES P C,JENKINSON D S.An exaction methodfor measuring soil microbial biomass C[J].Soil Biology&Biochem-istry,1987,19(6):703-707.
• [11]GUAN Song-yin.Soil enzyme and its study method[M].Beijing:A-gricultural Press,1986.关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
• [12]LI He-sheng.Plant physiology biochemistry experiment principleand technology[M].Beijing:Higher Education Press,2000.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
• [13]TAYLOR T P,WILSON B,MILLS M S,BURNS R G.Comparison ofmicrobial numbers and enzymatic activities in surface soils and sub-soils using various techniques[J].Soil Biology&Biochemistry,2002,34(3):387-401.
• [14]JIA Zhi-hong,YANG Zhen-ping,ZHANG Yong-qing,MIAO Guo-yuan.Study on the quantity of three main colony of soil microbe inwheat farmland[J].Journal of Triticeae Crops,2004,24(3):53-56.贾志红,杨珍平,张永清,苗果园.麦田土壤微生物三大类群数量的研究[J].麦类作物学报,2004,24(3):53-56.
• [15]FIDANZA M A,SANFORD DL,BEYER D M,DAVID J,AURENTZD J.Analysis of fresh mushroom compost[J].HortTechnology,2010,20(2):449-453.
• [16]RIBAS L C C,MENDONCA M M,CAMELINI C M,SOARES C H L.Use of spent mushroom substrates from Agaricus subrufescens(syn.A.blazei,A.brasiliensis)and Lentinula edodes productions in theenrichment of a soil-based potting media for lettuce(Lactuca sativa)cultivation:Growth promotion and soil bioremediation[J].BioresourceTechnology,2009,100(20):4750-4757.
• [17]JIANG Yong,LIANG Wen-ju,WEN Da-zhong.Effects of no-tillageon soil biological properties in farmlands[J].Chinese Journal of SoilScience,2004,35(3):347-351.姜勇,梁文举,闻大中.免耕对农田土壤生物学特性的影响[J].土壤通报,2004,35(3):347-351.
• [18]GAO Ming,ZHOU Bao-tong,WEI Chao-fu,XIE De-ti,ZHANG Lei.Effect of tillage system on soil animal,microorganism and enzymeactivity in paddy field[J].China Journal Applied Ecology,2004,15(7):1177-1181.高明,周保同,魏朝富,谢德体,张磊.不同耕作方式对稻田土壤动物、微生物及酶活性的影响研究[J].应用生态学报,2004,15(7):1177-1181.
• [19]LI Chun-xia,CHEN Fu,WANG Jun-zhong,LI You-jun.Effect ofdifferent tillage practices on soil enzyme activity[J].Chinese Journalof Soil Science,2007,38(3):601-603.李春霞,陈阜,王俊忠,李友军.不同耕作措施对土壤酶活性的影响[J].土壤通报,2007,38(3):601-603.
• [20]WANG Gui-fang,LI Bing-zhi,ZHANG Lin-sen,GUO Wen.Effectof application of biogas slurry with potassium on soil enzyme activityand quality of Red Fuji apple[J].Journal of Northwest Forestry Uni-versity,2009,24(5):88-91.王桂芳,李丙智,张林森,郭雯.苹果园沼液配施钾肥对土壤酶活性及果实品质的影响[J].西北北林学院学报,2009,24(5):88-91.
• [21]LI Si-jun,ZHONG Ze,LIU Xiang-rong,XIE Jian-zhuang,LI Guang-xian.Studies on the standard fertilizing formula and specific fertiliz-ers for apple trees in organic orchards[J].Journal of Fruit Science,2007,24(6):845-848.李四俊,钟泽,刘项荣,解建庄,李广献.苹果无公害标准化施肥方案及配套专用肥研究[J].果树学报,2007,24(6):845-848.
• [22]LI Bing-zhi,WANG Gui-fang,QIN Xiao-fei,ZHANG Lin-sen,HAN Ming-yu,ZHANG Li.Effect of application of biogas slurry withpotassium on orchard soil properties and soil microorganism and fruitquality[J].Scientia Agricultura Sinica,2010,43(22):4671-4677.李丙智,王桂芳,秦晓飞,张林森,韩明玉,张黎.沼液配施钾肥对果园土壤理化特性和微生物及果实品质影响[J].中国农业科学,2010,43(22):4671-4677.