龙泉1号天然三倍体枇杷的花粉形态及生活力Pollen morphology and viability of Longquan No. 1 natural triploid of loquat
郭启高;季昆;吴琼;何桥;武宇坤;李晓林;梁国鲁;
摘要(Abstract):
以龙泉1号枇杷的二倍体、天然三倍体株系为材料,应用扫描电镜进行了花粉形态的分析,并通过FDA荧光染色法、醋酸洋红染色、TTC法及离体萌发试验对花粉生活力进行测定,利用田间正反交试验,对其花粉在柱头上的自然萌发以及花粉管的生长过程进行观察。结果表明,(1)龙泉1号二倍体花粉属于N3P4C5类型,天然三倍体花粉有6种形态;(2)FDA染色表明15个龙泉1号三倍体枇杷株系的花粉生活力具有显著差异,且其测定的花粉生活力最接近实际的离体萌发率;(3)不同倍性植株的花粉在柱头中的生长主要取决于枇杷花粉的生活力,而与母本的倍性关系较小,且花粉管在柱头中为非匀速生长。
关键词(KeyWords): 枇杷;天然三倍体;花粉形态;花粉活力;花粉管
基金项目(Foundation): 国家自然科学基金(30971986);; 重庆市科委自然科学基金(9214)
作者(Author): 郭启高;季昆;吴琼;何桥;武宇坤;李晓林;梁国鲁;
Email:
DOI: 10.13925/j.cnki.gsxb.2010.03.011
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