郭启高;季昆;吴琼;何桥;武宇坤;李晓林;梁国鲁;

• 1:西南大学园艺园林学院

摘要(Abstract)：

以龙泉1号枇杷的二倍体、天然三倍体株系为材料,应用扫描电镜进行了花粉形态的分析,并通过FDA荧光染色法、醋酸洋红染色、TTC法及离体萌发试验对花粉生活力进行测定,利用田间正反交试验,对其花粉在柱头上的自然萌发以及花粉管的生长过程进行观察。结果表明,(1)龙泉1号二倍体花粉属于N3P4C5类型,天然三倍体花粉有6种形态;(2)FDA染色表明15个龙泉1号三倍体枇杷株系的花粉生活力具有显著差异,且其测定的花粉生活力最接近实际的离体萌发率;(3)不同倍性植株的花粉在柱头中的生长主要取决于枇杷花粉的生活力,而与母本的倍性关系较小,且花粉管在柱头中为非匀速生长。

关键词(KeyWords)： 枇杷;天然三倍体;花粉形态;花粉活力;花粉管

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30971986);; 重庆市科委自然科学基金(9214)

作者(Author): 郭启高;季昆;吴琼;何桥;武宇坤;李晓林;梁国鲁;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.03.011

参考文献(References)：

• [1]CHEN Zhen-guang,LIN Shun-quan,LIN Qing-liang.The primary introduction on obtaining loquat plants by endosperm in vitro[J].Journal of Fujian Agricultural Sciences,1983,12(4):343-346.陈振光,林顺权,林庆良.枇杷胚乳培养再生植株试验初报[J].福建农业学报,1983,12(4):343-346.
• [2]ZHANG Ze-huang,ZHENG Shao-quan,LIU You-jie.Studies on the seedless loquat fruit[J].Subtropical Plant Science,2002,31(1):9-13.张泽煌,郑少泉,刘友接.无籽枇杷研究初报[J].亚热带植物科学,2002,31(1):9-13.
• [3]SHENG Bao-long,WU Wei-min.Studies on the production of seed-less loquat[J].China Southern Fruits,1998,27(5):3-6.盛宝龙,吴伟民.无籽枇杷生产技术研究[J].中国南方果树,1998,27(5):3-6.
• [4]谷秀樹，段正幸,加藤彰宏,堀内昭作.ビワの無種子果実生産に関する研究(第2報)ジベレリン処理の低濃度化.園学雑.59別,1990(2):60-61.谷秀樹，段正幸,加藤彰宏,堀内昭作.无核枇杷果实生产的研究(第2报)低浓度赤霉素处理技术[J].园艺学会杂志59增刊(日本),1990(2):60-61.
• [5]LI Sun-kui,ZHUO Xiao-neng.The discussing on the effect of the radialization of Co60to loquat seeds[J].Fujian Fruit,1991(1):7-10.李孙逵,卓小能.钴60射线对枇杷种子辐射效应的探讨[J].福建果树,l991(1):7-10.
• [6]LIU Guo-qiang,LIN Zhi-qing,LIN Wen-zhong,LAI Zhong-xiong.A review of progress in study of Chinese loquat germplasm resource,selection and breeding[J].Subtropical Plant Research Communica-tion,2000,29(2):65-70.刘国强,林志清,林文忠,赖钟雄.我国枇杷种质资源及选育种研究进展[J].亚热带植物通讯,2000,29(2):65-70.
• [7]GUO Q G,LI X L,WANG W X,HE Q,LIANG G L.Occurrence of natural triploids in Loquat[J].Acta Horticulturae,2007,750,125-128.
• [8]HU Shi-yi.Experimental methods in plant embryology(Ⅰ)Determi-nation:of pollen viability[J].Chinese Bulletin of Botany,1993,10(2):60-62.胡适宜.植物胚胎学实验方法﹙一﹚花粉生活力的测定[J].生物学通报,1993,10(2):60-62.
• [9]WANG Ding-kang,SUN Gui-fang,GUO Zhi-ming,GUO Li-hong,CHEN Xue,GUO Qing.Comparison of determination methods of cy-nanchum otophllum schneid pollen activity[J].Journal of Anhui Agri-cultural Sciences,2008,36(12):5014,5016.王定康,孙桂芳,郭志明,郭丽红,陈雪,郭庆.青阳参花粉活力测定方法比较[J].安徽农业科学,2008,36(12):5104,5106.
• [10]HU Shi-yi.Method of preparation of slides used to examine the pollen germination on the stigma and pollen tube growth in the style[J].Chinese Bulletin of Botany,1994,11:58-60.胡适宜.植物胚胎学实验方法﹙五﹚检查花粉在柱头上萌发和花粉管在花拄中生长的制片法[J].植物学通报,1994,11:58-60.
• [11]ERDTMAN G.The handbook of pollen[M].Beijing:Beijing Science Press,1978.埃尔特曼.孢粉学手册[M].北京:北京科学出版社,1978.
• [12]ZHANG Hui-zhi,PENG Shu-ang,CAI Li-hong,FANG De-qiu.The germplasm resources of the genus Eriobotrya with special refer-ence on the origin of E.Japonica Lindl.[J].Acta Horticulturae Sini-ca,1990,17(1):5-12.章恢志,彭抒昂,蔡礼鸿,方德秋.中国枇杷属种质资源及普通枇杷起源研究[J].园艺学报,199017(1):5-12.
• [13]YANG Xiang-hui,WU Ying-xin,LIN Shun-quan.SEM observa-tiononthe pollenmorphologyofsix Eriobotrya plants[J].Journal of Fruit Science,2009,26(4):572-576.杨向晖,吴颖欣,林顺权.6种枇杷属植物花粉形态扫描电镜观察[J].果树学报,2009,26(4):572-576.
• [14]LU Xiu-min,CHEN Jing-ying,ZHANG Li-mei,ZHENG Shao-quan,YU Dong,LIAO Ru-yu.Observation and comparison on pollen morphology of a new hybrid loquat variety Zaozhong6and its parents[J].Acta Horticulturae Sinica,2002,29(3):271-273.陆修闽,陈菁瑛,张丽梅,郑少泉,余东,廖汝玉.枇杷杂交新品种早钟6号与亲本花粉形态观察比较[J].园艺学报,2009,29(3):271-273.
• [15]ZHANG Zi-xue,SUN Feng.The selection of the best testing method on the pollen viability in hot pepper[J].Seed,2002(1):32-33.张子学,孙峰.辣椒花粉生活力最佳测定方法的筛选[J].种子,2002(1):32-33.
• [16]Thomas Hunt Morgan,LU Hui-lin translate.The theory of the gene[M].Beijing:Peiking University Press,2007:95-98.摩尔根著,卢惠霖译.基因论[M].北京:北京大学出版社,2007:95-98.
• [17]GRANT V.Plant speciation[M].New York:Columbia University Press,1981.
• [18]HOLDAWAY-CLARKE T L,FEIJO J A,HACKETT GR.Pollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase while extracellular calcium influx is delayed[J].The Plant Cell,1997,9:1999-2010.