尚素琴;柳永花;刘宁;张彩虹;他光崇;

• 1:甘肃农业大学植物保护学院·甘肃省农作物病虫害生物防治工程实验室

摘要(Abstract)：

【目的】明确甲氰菊酯对苹果蠧蛾[Cydia pomonella(L.)]解毒酶系的影响。【方法】采用胃毒法测定甲氰菊酯对苹果蠹蛾3龄幼虫的LC_(10)和LC_(20)。以甲氰菊酯LC_(10)和LC_(20)质量浓度处理苹果蠧蛾3龄幼虫不同时间,测定其体内多功能氧化酶(MFOs)、羧酸酯酶(Car Es)和谷胱甘肽S-转移酶(GSTs)的活性变化。【结果】LC_(20)处理后,Car Es比活力在12、24、36、48 h时为对照的1.54、2.19、1.53和1.34倍,6 h和60 h时无明显变化;LC_(10)处理后,Car Es比活力在12、24、36 h时为对照的1.40、2.27和1.42倍。LC_(20)处理后GSTs比活力在12、24、36和48 h时均显著高于对照和LC_(10)处理(P<0.05);LC_(10)处理36 h和48 h时高于对照(P<0.05),其余时间均无明显变化。LC_(20)处理后,MFOs比活力在12、24和36 h时显著高于对照(P<0.05),48 h时显著低于对照(P<0.05),其余时间差异不显著;LC_(10)处理后12、24和36 h显著高于对照(P<0.05),其余时间差异均不显著。Car Es的K_m在LC_(20)处理后6、12、24、36、48和60 h分别比对照降低了40.00%、37.23%、32.17%、29.51%、52.94%和28.09%,V_(max)在6、12和48 h分别比对照升高了24.59%、19.36%和81.97%,差异显著(P<0.05);GSTs的K_m值和V_(max)变化趋势与Car Es相反;MFOs的K_m值在48 h显著低于对照(P<0.05),其他时间点无明显变化,V_(max)在12和48 h时无明显变化,其余时间点均显著低于对照(P<0.05)。【结论】LC_(10)和LC_(20)甲氰菊酯处理后的苹果蠹蛾体内Car Es、MFOs和GSTs均有解毒作用,但Car Es起主导作用。

关键词(KeyWords)： 苹果蠹蛾;甲氰菊酯;解毒酶;亚致死效应;抗药性

Abstract:

Keywords:

基金项目(Foundation): 甘肃省省级大学生创新创业训练计划(201610733002)

作者(Authors): 尚素琴;柳永花;刘宁;张彩虹;他光崇;

DOI: 10.13925/j.cnki.gsxb.20170280

参考文献(References)：

• [1]DENNI H S.Agricultural insect pests of temperate region sand their control[M].London:Cambridge University Press,1987:337-339.
• [2]秦晓辉,马德成,张煜,李广华,王培.苹果蠢蛾在我国西北发生危害情况[J].植物检疫,2006,20(2):49-51.QIN Xiaohui,MA Decheng,ZHANG Yu,LI Guanghua,WANG Pei.The harmfulness of Cydia pomonella in the northwest China[J].Plant Quarantine,2006,20(2):49-51.
• [3]杨建强,赵骁,严勇敢,张雅林,冯纪年.7种药剂对苹果蠹蛾的防治效果[J].西北农业学报,2011,20(9):191-196.YANG Jianqiang,ZHAO Xiao,YAN Yonggan,ZHANG Yalin,FENG Jinian.Efficacy of seven kinds of pesticides for controlling codling moth Cydia pomonella[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(9):191-196.
• [4]DAVID G,JAMES S,TANYA S.Fecundity in two spotted spider mite(Acari:Tetranychidae)is increased by direct and systemic exposure to imidacloprid[J].Journal of Economic Entomology,2002,95(4):729-732.
• [5]NEMOTO H.Mechanism of resurgence of the diamondback moth,Plutella xylostella(L.)(Lepidoptera:Yponomeutidae)[J].Japan Agriculture Research Quarterly,1993,27(1):27-30.
• [6]MOORE A,TABASHNIK B E,RETHWISCH M D.Sublethal effects of fenvalerate on adults of the diamomdback moth(Lepidoptera:Plutellidae)[J].Journal of Economic Entomology,1992,85(5):1624-1627.
• [7]PERVEEN F.Sublethal effects of chlorfluazuron on reproductivity and viability of Spodoptera litura(F.)(Lep.,Noctuidae)[J].Journal of Applied Entomology,2000,124(5/6):223-231.
• [8]张自启,仲嘉伟,韩瑞华,刘长营.氯虫苯甲酰胺对二点委夜蛾的亚致死效应研究[J].西南农业学报,2014,27(5):1949-1952.ZHANG Ziqi,ZHONG Jiawei,HAN Ruihua,LIU Changying.Sublethal effects of chlorantraniliprole on Athetis lepigone[J].Southwest China Journal of Agricultural Sciences,2014,27(5):1949-1952.
• [9]柏敏战,郑贵彬.番茄枯萎抗病性室内鉴定方法研究[J].植物病理学报,1997,27(1):59-63.BAI Minzhan,ZHENG Guibin.A method for determination of resistance to tomato fusarium with in greenhouse[J].Acta Phytopathologica Sinica,1997,27(1):59-63.
• [10]沈慧敏.苹果黄蚜抗甲氰菊酯种群对13种杀虫剂的交互挤性研究[J].农药,1998(1):20-22.SHEN Huimin.Study on cross-resistance of fenpropathrin-resistant strains of the Aphis citricold to 13 pesticides[J].Pesticides,1998(1):20-22.
• [11]何玉仙,黄建.烟粉虱抗药性的研究进展[J].生物安全学报,2005,14(4):336-342.HE Yuxian,HUANG Jian.Advance of insecticide resistance in Bemisia tabaci[J].Journal of Biosafety,2005,14(4):336-342.
• [12]DUNLEY J E,WELTER S C.Correlated insecticide cross-resistance in azinphosmethyl resistant codling moth(Lepidoptera:Tortricidae)[J].Journal of Economic Entomology,2000,93(3):955-962.
• [13]REYES M,SAUPHANOR B.Resistance monitoring in codling moth:a need for standardization.[J].Pest Management Science,2008,64(9):945-953.
• [14]BOUVIER J C,CUANY A,MONIER C,BROSSE V,SAUPHANOR B.Enzymatic diagnosis of resistance to deltamethrin in diapausing larvae of the codling moth,Cydia pomonella,(L.)[J].Archives of Insect Biochemistry&Physiology,1998,39(2):55-64.
• [15]KONANZ S.Characterization of mechanisms of resistance to common insecticides in noctuid pest species and resistance risk assessment for the new lepidopteran specific compound flubendiamide[D].Stuttgart:University Hohenheim,2009.
• [16]BRADFORD W W.A rapid and sensitive method for the quantization of microgram quantities of protein utilization the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1):248-254.
• [17]何林,谭仕禄,曹小芳,赵志模,邓新平,王进军.朱砂叶螨的抗药性选育及其解毒酶活性研究[J].农药学学报,2003,5(4):23-29.HE Lin,TAN Shilu,CAO Xiaofang,ZHAO Zhimo,DENG Xinping,WANG Jinjun.Study on resistance selection and activity of detoxification enzyme in Tetranychus cinnabarinus[J].Chinese Journal of Pesticide Science,2003,5(4):23-29.
• [18]何恒果.桔全爪螨对甲氰菊酯和阿维菌素的抗性及其酯酶基因的克隆与表达研究[D].重庆:西南大学,2010.HE Hengguo.Fenpropathrin and avermectin resistance,and esterasen gene cloning and expression in Panonychus citri(McGregor)[D].Chongqing:Southwest University,2010.
• [19]CLARK A G,DICK G L,SMITH J N.Kinetic studies on a glutathione S-transferase from the larvae of Costelytra zealandica[J].Biochemistry,1984,217(1):51-58.
• [20]HABIG W H,PABST M J,JAKOBY W B.Glutathiones S-transferases the first enzymatic step in mercapturic acid formation[J].The Journal of Biological Chemistry,1974,249(22):7130-7139.
• [21]KIM Y J,PARK H M,CHO J R,AHN Y J.Multiple resistance and biochemical mechanisms of pyridaben resistance in Tetranychus urticate(Acari:Tetranychidae)[J].Journal of Economic Entomology,2006,99(3):954-958.
• [22]WILKINSON G N.Statistical estimations in enzyme kinetics[J].Biochemical Journal,1961,80(2):324-332.
• [23]尹飞,冯夏,李振宇,林庆胜,胡珍娣,张德雍,陈焕瑜.亚致死剂量氯虫苯甲酰胺对小菜蛾体内活性酶的影响[J].植物保护,2014,40(2):66-69.YIN Fei,FENG Xia,LI Zhenyu,LIN Qingsheng,HU Zhendi,ZHANG Deyong,CHEN Huanyu.Effects of sublethal concentrations of chlorantraniliprole on detoxifying enzyme activity in Plutella xylostella(L.)[J].Plant Protection,2014,40(2):66-69.
• [24]唐振华,毕强.杀虫剂作用的分子行为[M].上海:上海远东出版社,2003:675.TANG Zhenhua,BI Qiang.Molecular behavior of insecticides action[M].Shanghai:Shanghai Far East Press,2003:675.
• [25]唐振华.昆虫抗药性及其治理[M].北京:农业出版社,1993.TANG Zhenhua.Insecticide resistance and treatment of insects[M].Beijing:Agricultural Press,1993.
• [26]陈列忠,王开金,陈建明,吕仲贤,郑许松,徐红星,张珏锋,俞晓平.雷公藤生物碱对小菜蛾幼虫生长及其解毒酶系的影响[J].生物安全学报,2005,14(3):238-242.CHEN Liezhong,WANG Kaijin,CHEN Jianming,LüZhongxian,ZHENG Xusong,XU Hongxing,ZHANG Juefeng,YU Xiaoping.Effect of tripterygium wilfordii alkaloids on diamondback moth(Plutella xylostella)larval growth and 2 detoxification enzyme activities[J].Journal of Biological Safety,2005,14(3):238-242.
• [27]尹显慧,吴青君,李雪锋,张友军,徐宝云.多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响[J].农药学学报,2008,10(1):28-34.YIN Xianhui,WU Qingjun,LI Xuefeng,ZHANG Youjun,XU Baoyun.Effect of sublethal concentrations of spinosad on the activities of detoxifying enzymes in the larvae of diamondback moth Plutella xylostella[J].Chinese Journal of Pesticide Science,2008,10(1):28-34.
• [28]孟和生,王开运,姜兴印,仪美芹.桔全爪螨的抗药性选育及其解毒酶活力变化[J].昆虫学报,2002,45(1):58-62.MENG Hesheng,WANG Kaiyun,JIANG Xingyin,YI Meiqin.Studies on resistance selection by abamectin and fenpropathrin and activity change of detoxicant enzymes in Panonychus citri[J].Acta Entomologica Sinica,2002,45(1):58-62.