李芳菲;王莎;谷世超;程大伟;顾红;李明;陈锦永;杨英军;

• 1:河南科技大学林学院
• 2:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】研究叶面喷施脱落酸(ABA)和二氢茉莉酸丙酯(PDJ)对‘巨峰’葡萄果实着色及品质的影响。【方法】在果实转色初期和中期,使用不同浓度ABA(A1:10 mg·L~(-1)、A2:25 mg·L~(-1)、A3:50 mg·L~(-1))和PDJ(P1:5 mg·L~(-1)、P2:10mg·L~(-1)、P3:25 mg·L~(-1))对‘巨峰’葡萄进行叶面喷施处理,并分析不同处理下成熟果实花色苷、叶绿素、类胡萝卜素、可溶性固形物、可滴定酸和类黄酮含量,以及穗重、单粒重等指标的差异,通过主成分分析对葡萄果实着色指标和内在品质进行综合评价,以确定最适宜的ABA和PDJ施用浓度。【结果】不同浓度ABA和PDJ处理均能显著增加果皮花色苷含量,降低叶绿素和类胡萝卜素含量,提高了颜色指数(CIRG)和红绿色差指标(a~*),并降低果实亮度(L~*)和黄蓝色差指标(b~*),从而提高果实着色程度;果穗重、单粒重增大,可溶性固形物和果皮类黄酮含量显著上升,但对果实可滴定酸无影响。【结论】不同处理的效果综合评价依次为A2>P2>A1>A3>P1>P3>CK,在所有处理中,A2处理对于促进果实着色以及提高果实品质的效果最好,P2处理次之,ABA处理效果整体上优于PDJ处理。

关键词(KeyWords)： ‘巨峰’葡萄;脱落酸(ABA);二氢茉莉酸丙酯(PDJ);果实着色;品质

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金—葡萄细胞分裂素响应调节因子VvRR2在坐果与幼果发育中的功能分析(31701893);; 中央级公益性科研院所基本科研业务费专项(1610192019502);; 河南省大宗水果产业技术体系(S2014-11)

作者(Author): 李芳菲;王莎;谷世超;程大伟;顾红;李明;陈锦永;杨英军;

Email:

DOI: 10.13925/j.cnki.gsxb.20190551

参考文献(References)：

• [1]国家葡萄产业技术体系育种研究室.植物生长调节剂在葡萄生产中的应用[M].北京：中国农业出版社，2011.Breeding Research Laboratory of National Crape Industry Technology System. Application of plant growth regulator in grape production[M]. Beijing:China Agriculture Press,2011.
• [2]黄璐.巨峰葡萄不同芽节位夏季花芽分化规律及相关影响因子的研究[D].南宁：广西大学，2015.HUANG Lu. Study on the summer differentiation of buds in different nodes and its influence factors in Kyoho grape[D].Nanning:Guangxi University,2015.
• [3]陈锦永，顾红，赵长竹，张威远，魏世忠. ABA促进巨峰葡萄着色和成熟试验简报[J].中外葡萄与葡萄酒，2010(1):43-44.CHEN Jinyong,GU Hong,ZHAO Changzhu,ZHANG Weiyuan,WEI Shizhong. The test briefing of ABA promoting grape coloring and maturity[J]. Sino-Overseas Grapevine&Wine,2010(1):43-44.
• [4] NAMBARA E,MARION-POLL A. Abscisic acid biosynthesis and catabolism[J]. Annual Review of Plant Biology,2005,56(1):165-185.
• [5]马文瑶，程大伟，顾红，黄海娜，陈锦永，杨英军.脱落酸(ABA)促进果实着色研究进展[J].果树学报，2018,35(8):1016-1026.MA Wenyao,CHENG Dawei,GU Hong,HUANG Haina,CHEN Jinyong,YANG Yingjun. Advances in ABA promoting fruit coloration[J]. Journal of Fruit Science,2018,35(8):1016-1026.
• [6]于淼.脱落酸和乙烯利对葡萄花色苷生物合成相关基因表达的影响[D].哈尔滨：东北林业大学，2010.YU Miao. Effects of abscisic acid and ethephon on expression of genes related to anthocyanin biosynthesis in grapevine[D].Ha'erbin:Northeast Forestry University,2010.
• [7]李云鹏. ABA和BR及其复合剂对葡萄果实着色的效应及机理研究[D].沈阳：沈阳农业大学，2003.LI Yunpeng. Effect and mechanism of ABA,BR and its compound on fruit coloring of grape[D]. Shenyang:Shenyang Agricultural University,2003.
• [8]王芳，陈子林.茉莉酸类植物激素分析研究进展[J].生命科学，2010,22(1):45-58.WANG Fang,CHEN Zilin. Advance in the analysis of plant hormone jasmonates[J]. Chinese Bulletin of Life Sciences,2010,22(1):45-58.
• [9] YU Y H,JIAO Z L,BIAN L,WAN Y R,YU K K,ZHANG G H,GUO D L. Overexpression of Vitis vinifera VvbZIP60 enhances Arabidopsis resistance to powdery mildew via the salicylic acid signaling pathway[J]. Scientia Horticulturae,2019,256:108640.
• [10] KONDO S,TOMIYAMA A,SETO H. Changes of endogenous jasmonic acid and methyl jasmonate in apples and sweet cherries during fruit development[J]. Journal of the American Society for Horticultural Science,2000,125(3):282-287.
• [11] PORTU J,SANTAMARíA P,LóPEZ-ALFARO I,LóPEZ R,GARDE-CERDáN T. Methyl jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content[J]. Journal of Agricultural&Food Chemistry,2015,63(8):2328.
• [12]徐后娟，丁秀英，刘峰，张军.二氢茉莉酸丙酯对烟草黑胫病的控制作用及对osmotin基因表达的影响[J].中国烟草学报，2005,11(5):31-34.XU Houjuan,DING Xiuying,LIU Feng,ZHANG Jun. Effects of propyl dihydrojasmonate on tobacco black shank and its effect on osmotin gene expression[J]. Acta Tabacaria Sinica,2005,11(5):31-34.
• [13]马文瑶，程大伟，黄海娜，陈锦永，杨英军. PDJ对‘巨玫瑰’葡萄果实着色及品质的影响[J].中国农业科技导报，2019,21(3):118-125.MA Wenyao,CHENG Dawei,HUANG Haina,CHEN Jinyong,YANG Yingjun. Effect of prohydrojasmon on coloration and quality of the‘Jumeigui’grape berry[J]. Journal of Agricultural Science and Technology,2019,21(3):118-125.
• [14]马焕普，陈静，刘志民，梁宝岩.天然芸薹素和茉莉酸酯对葡萄果实品质及成熟期的影响[J].北方果树，2004(4):8-9.MA Huanpu,CHEN Jing,LIU Zhimin,LIANG Baoyan. Effects of natural brassinolide and jasmonate on the quality and ripening stage of grape fruits[J]. Northern Fruits,2004(4):8-9.
• [15]孟祥云，王枝翠，王雨歌，樊新民，赵宝龙，刘怀锋.地面遮阴对新疆‘红地球’葡萄果实着色的影响[J].果树学报，2014,31(1):60-65.MENG Xiangyun,WANG Zhicui,WANG Yuge,FAN Xinmin,ZHAO Baolong,LIU Huaifeng. Effects of terrestrial shading on the berry coloring of‘Red Globe’grape(Vitis vinifera L.)in Xinjang[J]. Journal of Fruit Science,2014,31(1):60-65.
• [16] AMIRI M E,FALLAHI E,PARSEH S. Application of ethephon and ABA at 40%veraison advanced maturity and quality of‘Beidaneh Ghermez’grape[J]. International Symposium on Plant Bioregulators in Fruit Production,2010,(884):371-377.
• [17]孙晓文.茉莉酸酯类对葡萄果实着色及品质的影响[D].北京：中国农业科学院，2016.SUN Xiaowen. Effects of jasmonates on coloration and quality of grape berry[D]. Beijing:Chinese Academy of Agricultural Sciences,2016.
• [18] MEYERS K J,WATKINS C B,PRITTS M P,LIU R U. Antioxidant and antiproliferative activities of strawberries[J]. Journal of Agricultural&Food Chemistry,2003,51(23):6887-6892.
• [19] HE J J,LIU Y X,PAN Q H,CUI X Y,DUAN C Q. Different anthocyanin profiles of the skin and the pulp of Yan7(Muscat Hamburg×Alicante Bouschet)grape berries[J]. Molecules,2010,15(3):1141-53.
• [20]刘洪海，张晓丽，杜平，张希波. pH示差法测定烟73葡萄中花青素含量[J].中国调味品，2009,34(4):110-111.LIU Honghai,ZHANG Xiaoli,DU Ping,ZHANG Xibo. Determination the anthocyanin content in Yan73 grape by pH-differential method[J]. China Condiment,2009,34(4):110-111.
• [21]王延书.葡萄果实着色前后ABA代谢合成关键酶的基因表达分析[D].泰安：山东农业大学，2011.WANG Yanshu. Transcriptional analysis of key enzymes for ABA metabolism and biosynthesis around the veraison of grape berry ripening[D]. Tai’an:Shandong Agricultural University,2011.
• [22]马文婷.脱落酸、乙烯利和芸薹素内酯对蛇龙珠葡萄果实品质及果皮花色苷的影晌[D].银川：宁夏大学，2015.MA Wenting. Effects of ABA,ETH and BR on fruit quality and anthocyanin on grape peel of Cabernet Gernischt[D]. Yinchuan:Ningxia University,2015.
• [23]程云，吴欣欣，李百健，高志红.外源脱落酸对魏可葡萄果实着色及品质的影响[J].江苏农业科学，2014,42(10):163-166.CHENG Yun,WU Xinxin,LI Baijian,GAO Zhihong. Effect of exogenous abscisic acid on coloration and quality of Weike grape[J]. Jiangsu Agricultural Sciences,2014,42(10):163-166.
• [24]李芳菲，马文瑶，程大伟，黄海娜，顾红，陈锦永，杨英军.植物生长调节物质对葡萄着色影响的研究进展[J].果树学报，2019,36(7):928-938.LI Fangfei,MA Wenyao,CHENG Dawei,HUANG Haina,GU Hong,CHEN Jinyong,YANG Yingjun. Advances in grape coloration by plant growth regulators[J]. Journal of Fruit Science,2019,36(7):928-938.
• [25]王鹏洋，曲姗姗. ABA对果实品质的影响研究进展[J].现代农业科技，2019(7):198.WANG Pengyang,QU Shanshan. Advances in effects of ABA on fruit quality[J]. Modern Agricultural Sciences and Technology,2019(7):198.
• [26]谢周.根域限制和不同植物生长调节剂对葡萄生长发育的影响[D].南京：南京农业大学，2010.XIE Zhou. The effects of root restriction and different plant growth regulators on the development of grapevines[D]. Nanjing:Nanjing Agricultural University,2010.
• [27] KOYAMA R,ROBERTO S R,DE SOUZA R T,BORGES W F S,ANDERSON M,WATERHOUSE A L,CANTU D,FIDELIBUS M W,BLANCO-ULATE B. Exogenous abscisic acid promotes anthocyanin biosynthesis and increased expression of flavonoid synthesis genes in Vitis vinifera×Vitis labrusca table grapes in a Subtropical Region[J]. Frontiers in Plant Science,2018,9:323.
• [28]何平.茉莉酸酯类对秋甜桃果实着色及品质的影响[J].分子植物育种，2019,17(7):2371-2378.HE Ping. Effects of jasmonates on coloration and quality of qiutian peach[J]. Molecular Plant Breeding,2019,17(7):2371-2378.
• [29] ATAY E. Effect of application time of prohydrojasmon on fruit coloration of Gala and Braeburn apples[J]. Bulgarian Journal of Agricultural Science,2015,21(6):1242-1247.
• [30]任俊鹏，李小红，董瑞奇，宋新新，顾克余，沈月芳，陶建敏.环剥和脱落酸处理对‘夏黑’葡萄果实着色及相关基因表达的影响[J].果树学报，2013 30(6):968-974.REN Junpeng,LI Xiaohong,DONG Ruiqi,SONG Xinxin,GU Keyu,SHEN Yuefang,TAO Jianmin. Effect of girdling and ABA treatment on fruit quality and peel coloring of‘Summer Black’grape[J]. Journal of Fruit Science,2013,30(6):968-974.
• [31] JEONG S T,GOTO-YAMAMOTO N,KOBAYASHI S,ESAKA M. Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins[J]. Plant Science,2004,167(2):247-252.
• [32]闵卓，欧阳亚南，张阳，刘敏，鞠延仑，房玉林.葡萄与葡萄酒中类黄酮物质的研究进展[J].北方园艺，2018(5):160-170.MIN Zhuo,OUYANG Yanan,ZHANG Yang,LIU Min,JU Yanlun,FANG Yulin. Advances in flavonoids in grape and wine[J].Northern Horticulture,2018(5):160-170.