菠萝种质鉴定及亲缘关系的AFLP分析Identification of pineapple germplasm and relationship analysis with AFLP
刘卫国,易干军,刘岩,张秋明,曾继吾
摘要(Abstract)：
从FISH-AFLP试剂盒(PSTI/MseI型)64对引物中,筛选出8对AFLP选择性引物P-GAA/M-CTA、P-GAC/M-CTG、P-GAC/M-CTT、P-GAG/M-CAT、P-GAG/M-CTA、P-GAT/M-CAT、P-GAT/M-CTA、P-GAT/M-CTC,对39份菠萝种质进行了AFLP分析,都得到了清晰的多态性指纹图谱。8对引物共获得454个遗传位点,其中多态性位点332个,多态性比例平均为73.1%。UPGMA聚类表明,各种质间的相似系数为0.73～0.98,说明39份菠萝种质遗传关系相对较近。依相似系数0.80的水平,将供试的39份菠萝种质分为4类。研究结果可以作为菠萝分类的理论依据和基础。
关键词(KeyWords)： 菠萝;亲缘关系;AFLP
基金项目(Foundation): 广东省科技厅农业攻关项目(2003C201005)
作者(Author): 刘卫国,易干军,刘岩,张秋明,曾继吾
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