王春林;张玉鑫;陈年来;代春艳;方春媛;康恩祥;

• 1:甘肃农业大学农学院
• 2:陇东学院
• 3:甘肃农业大学资源与环境学院

摘要(Abstract)：

通过对含有苯并噻二唑(BTH)、咖啡酸(CA)或氯化钴(CC)等不同MS培养上甜瓜愈伤组织的研究,测定了超氧阴离子(O2■)产生速率、过氧化氢(H2O2)含量及抗性相关酶活性的变化,探讨了BTH诱导的甜瓜ROS代谢和抗性酶活性及其相关的信号途径。结果表明,CA可以消除BTH对甜瓜愈伤组织O2■增加和H2O2积累的诱导作用;CA或CC能够部分抵消BTH对SOD增强的诱导作用;CC对BTH诱导的POD增强具有抑制效应,CA+CC可完全消除BTH对POD的诱导作用;CA和CC均能促进BTH对PAL增强的诱导作用,且具有协同效应。BTH对甜瓜O2■升高、H2O2积累和CAT降低的诱导主要通过SA途径实现,对POD的影响主要通过ET途径实现,对SOD升高的诱导通过SA和ET 2条途径实现,而对PAL活性的影响通过ET和SA以外的途径实现。

关键词(KeyWords)： 甜瓜;苯并噻二唑;咖啡酸;氯化钴;活性氧;抗病相关酶;信号途径

Abstract:

Keywords:

基金项目(Foundation): 甘肃省农业生物技术研究与应用开发项目(GNSW-2006-06)

作者(Author): 王春林;张玉鑫;陈年来;代春艳;方春媛;康恩祥;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.02.019

参考文献(References)：

• [1]LAMB C,DIXON R A.The oxida tive burst in plant disease resis-tance[J].Annual Review of Plant Physiology and Plant Molecular Bi-ology,1997,48:251-275.
• [2]WANG Luo-xia,ZHANG Sheng-min,CHEN Yin-hua.Roles of re-active oxygen species and nitric oxide in interactions between plantsand pathogens[J].Chinese Journal of Tropical Agriculture,2009,29(6):70-77.王罗霞,张盛敏,陈银华.活性氧和一氧化氮在植物-病原体互作反应中的作用[J].热带农业科学,2009,29(6):70-77.
• [3]DONG Hai-li,JING Jin-xue.Role of ROS and NO in plant diseaseresistance responses[J].Journal of Northwest A&F University:Nat-ural Science Edition,2003,31(1):161-166.董海丽,井金学.活性氧和一氧化氮在植物抗病反应中的作用[J].西北农林科技大学学报:自然科学版,2003,31(1):161-166.
• [4]WU Qing-li,TAN Xiao-rong.Role of reactive oxygen species incrop disease resistance[J].Journal of Henan University of Technology:Natural Science Edition,2008,29(3):81-86.吴庆丽,谭晓荣.活性氧在农作物抗病中的作用[J].河南工业大学学报:自然科学版,2008,29(3):81-86.
• [5]ZHOU Song,YI Jing.Ligand-induced generation of reactive oxygenspecies in cell signaling[J].Chinese Journal of Cell Biology,2006,28(5):656-660.周崧,易静.信号配体诱导的活性氧生成[J].细胞生物学杂志,2006,28(5):656-660.
• [6]PAN Xiao-wu,LI Yong-chao,LI Xiao-xiang,HUANG Rong-feng.Metabolism and signal transduction of H2O2 in plants under abioticstresses[J].Journal of Agricultural Science and Technology,2010,12(2):38-43.潘孝武,黎用朝,李小湘,黄荣峰.非生物胁迫条件下植物H2O2的代谢及信号转导[J].中国农业科技导报,2010,12(2):38-43.
• [7]ZHAO Li-kun,LIAO Xiang-ru,JIANG Ji-zhi.Study advance inactive oxygen and plant systemic acquired resistance[J].Journal ofAgricultural University of Hebei,2002,25:173-176.赵丽坤,廖祥儒,蒋继志.活性氧与植物系统获得抗病性研究进展[J].河北农业大学学报,2002,25:173-176.
• [8]KUNKEL B N,BROOKS D M.Cross talk between signaling pathwaysin pathogen defense[J].Current Opinion in Plant biology,2002,5(4):325-331.
• [9]STVEN H S,ANNEMART K,SUSANNE M C C.NPR1 modulatescross-talk between salicylate-and jasmonate-dependent defensepathways through a novel function in the cytosol[J].The Plant Cell,2003,15:760-770.
• [10]BERNARDS M A,STRACK D,WRAY V,ELLIS B E.Caffeoyl glu-cosides in fungal challenged tomato suspension cultures[J].Phyto-chemistry,1991,30(2):497-499.
• [11]YAN Wen-wen,LI Yan-fang,HE Li-hong,BIN Jin-hua.Effect ofcafeic acid and cobalt chloride on the defense related enzymes oftobacco cain inducing by methyl jasmonate[J].Chinese Bulletin ofBotany,2003,20(1):67-74.严文文,李艳芳,贺立红,宾金华.咖啡酸和氯化钴对茉莉酸甲酯诱导抗病相关酶活性的影响[J].植物学通报,2003,20(1):67-74.
• [12]FAN Wen-jing,CUI Xin-yi,WANG Xue-li.Progresses of PlantActivator Benzothiadiazole[J].Journal of Tianjin Agricultural Uni-versity,2009,16(3):52-55.范文静,崔新仪,王学利.植物激活剂苯并噻二唑(BTH)的研究进展[J].天津农学院学报,2009,16(3):52-55.
• [13]CHEN Nian-lai,HU Min,QIAO Chang-ping,NAI Xiao-ying,WANG Rui.Effects of BTH,SA,and SiO2treatment on disease re-sistance and leaf HRGP and lignin contents of melon seedlings[J].Scientia Agricultura Sinica,2010,43(3):535-541.陈年来,胡敏,乔昌萍,乃小英,王锐.BTH、SA和SiO2处理对甜瓜幼苗白粉病抗性及叶片HRGP和木质素含量的影响[J].中国农业科学,2010,43(3):535-541.
• [14]CHEN Nian-lai,ZHU Zhen-jia,DAI Chun-yan,WANG Chun-lin,AN Cui-xiang.Induction of resistance of melon cultivars withdifferent resistances to powdery mildew after BTH treatment[J].Journal of Fruit Science,2010,27(5):764-769.陈年来,朱振家,代春艳,王春林,安翠香.BTH诱导不同抗性水平甜瓜抗白粉病的效应[J].果树学报,2010,27(5):764-769.[15]DAI Chun-yan,ZHU Zhen-jia,AN Cui-xiang,CHEN Nian-lai.Powdery mildew resistant enzyme activities induced with BTH in melon seedlings[J].Jiangsu J of Agr Sci,2010,26(2):286-291.代春艳,朱振家,安翠香,陈年来.诱抗剂BTH诱导甜瓜幼苗抗白粉病相关酶活性的研究[J].江苏农业学报,2010,26(2):286-291.[16]MA Ke-qi,CHEN Nian-lai,WANG Ming.Melon cultivation theory and practice[M].Beijing:China Agricultural Press,2001,111-117.马克奇,陈年来,王鸣.甜瓜优质栽培理论与实践[M].北京:中国农业出版社,2001,111-117.[17]ZONG Hui,HU Wen-yu,CHEN Gui.Determination of superoxide anion radicals content in fruits by the method of hydrorylamine oxidization[J].Journal of Shenyang Agricultural University,1998,29(4):326-329.宗会,胡文玉,陈贵.羟胺氧化法测定果实中O 2.含量方法[J].沈阳农业大学报,1998,29(4):326-329.[18]LIN Zhi-fang,LI Shuan-shun,LIN Gui-zhu,GUO Jun-yan.The Accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation[J].Acta Phytophysiologica Sinica,1988,14(1):16-22.林植芳,李双顺,林桂珠,郭俊彦.衰老叶片和叶绿体中H 2 O 2的积累与膜脂过氧化的关系[J].植物生理学报,1988,14(1):16-22.
• [19]LI He-sheng.Plant Physiology and Biochemistry experimental prin-ciple and technology[M].Beijing:Higher Education Press,2000,213-214.李合生.植物生理生化试验原理与技术[M].北京:高等教育出版社,2000,167-169.
• [20]ZOU Qi.Plant Physiology experimental guide[M].Beijing:China A-gricultural Press,2001:57-58.邹琦.植物生理学实验指导[M].北京:中国农业出版社,2001:57-58.
• [21]CHEN Nian-lai,ZHU Zhen-jia,AN Cui-xiang,HU Min.Effects of chemical elicitors on several defense-related enzyme activities in muskmelon leaves[J].Acta Botanica Boreali-Occidentalia Sinica,2008,28(7):1354-1358.陈年来,朱振家,安翠香,胡敏.诱抗处理对甜瓜叶片防卫酶活性的影响[J].西北植物学报,2008,28(7):1354-1358.
• [22]CAO S F,ZHENG Y H,YANG Z F,TANG S S,JIN P,WANG K T,WANG X M.Effect of methyl jasmonate on the inhibition of Col-letotrichum acutatum infection in loquat fruit and the possible mech-anisms[J].Postharvest Biology and Technology,2008,49(2):301-307.
• [23]IWANO M,CHE F S,GOTO K,TANAKA N,TAKAYAMA S,ISOGAI A.Electron microscopic analysis of the H 2 O 2 accumulation preceding hypersensitive cell death induced by an incompatible strain of Pseudomonas avenae in cultured rice cells[J].Molecular Plant Pathology,2002,3(1):1-8.