张志宏;李丽丽;杨洪一;

• 1:沈阳农业大学园艺学院
• 2:沈阳农业大学园艺学院
• 3:沈阳农业大学园艺学院 沈阳110161
• 4:沈阳110161

摘要(Abstract)：

依赖核酸序列的扩增(Nucleic acid sequence based amplification,NASBA)技术是以cDNA为中介体在等温条件下直接扩增单链RNA特异序列的核酸扩增技术,研究探索利用NASBA技术检测草莓斑驳病毒(Strawberry mottle virus,SMoV)。在对14个SMoV分离物序列分析的基础上,设计和筛选出适于SMoV检测的NASBA引物。NASBA检测SMoV的适宜反应温度为40℃。在9份草莓样品上检测SMoV的结果显示,NASBA检测结果与RT-PCR的检测结果一致,这表明初步建立了利用NASBA技术检测SMoV的技术体系。这是利用NASBA检测SMoV的首次报道。

关键词(KeyWords)： 草莓;斑驳病毒;NASBA;检测

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30200187)

作者(Author): 张志宏;李丽丽;杨洪一;

Email:

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