王晓芳;相昆;孙岩;崔冬冬;亓雪龙;王来平;翟衡;李勃;

• 1:山东省果树研究所
• 2:山东农业大学园艺科学与工程学院

摘要(Abstract)：

【目的】探究光氧化胁迫条件下叶面肥对葡萄叶片的保护及缓解效应。【方法】以设施栽培的‘巨峰’葡萄为试材,叶面喷施CaCl_2、NaHSO_3、氨基酸钙叶面肥、氨基酸钙叶面肥+NaHSO_3,然后叶片涂抹甲基紫精(MV)模拟光氧化胁迫,研究叶面肥对光氧化条件下葡萄叶片的光合、荧光参数、超氧阴离子(O_2~)产生速率、H_2O_2含量及MDA含量等的影响。【结果】叶片涂抹MV后发生光氧化胁迫,各处理的相关指标均发生改变,其指标表现均不如仅喷清水的对照,但与喷清水+MV处理相比,喷施叶面肥处理指标变化幅度均变小,不同程度地缓解了光氧化对葡萄叶片的伤害。喷清水+MV处理的净光合速率(P_n)下降幅度最大,比清水对照下降了47.9%,喷施氨基酸钙肥600倍液以及氨基酸钙肥600倍液+NaHSO_3的效果好,P_n下降幅度小,分别比喷清水+MV处理的低34.8个百分点和33.7个百分点,差异显著;气孔导度(G_s)变化趋势同P_n。初始荧光(F_0)指标,喷清水+MV处理增幅最大,为22.0%;喷施氨基酸钙600倍液+NaHSO_3处理的增幅最小,为5.1%,比前者低16.9个百分点;最大荧光F_m、PSⅡ最大光化学效率F_v/Fm、F_v/F_0、实际光化学效率Φ_(PSⅡ),喷施叶面肥处理下降幅度均小于喷清水+MV处理。各处理的超氧阴离子(O_2~)产生速率、H_2O_2含量、MDA含量增加幅度不同,其中喷清水+MV处理增加幅度最大,分别为56.0%、83.8%、34.8%;喷施叶面肥处理增加幅度均变小,其中喷施氨基酸钙600倍液+NaHSO_3处理的增加幅度最小,分别为19.4%、15.1%、0.99%,明显减轻了光氧化胁迫。【结论】综合各项指标,喷施叶面肥对葡萄光氧化胁迫有明显的缓解效应,以喷施氨基酸钙600倍液+NaHSO_3效果最好,建议在生产中选择应用。

关键词(KeyWords)： ‘巨峰’葡萄;光氧化;叶面肥;光合作用;叶绿素荧光;MDA;H2O2

Abstract:

Keywords:

基金项目(Foundation): 国家现代葡萄产业技术体系建设专项(CARS-30);; 泰安市科技发展计划(2014NS54)

作者(Author): 王晓芳;相昆;孙岩;崔冬冬;亓雪龙;王来平;翟衡;李勃;

Email:

DOI:

参考文献(References)：

• [1]李俊,晁赢,官春云,马霓,张春雷.甘蓝型油菜幼苗对光氧化胁迫的生理响应[J].中国油料作物学报,2013,35(5):551-556.LI Jun,CHAO Ying,GUAN Chunyun,MA Ni,ZHANG Chunlei.Response of physiological changes in rapeseed seedling under oxidative stress[J].Chinese Journal of Oil Crop Sciences,2013,35(5):551-556.
• [2]邵玉娇.不同光强下油菜品质形成的生理基础研究[D].武汉:华中农业大学,2005.SHAO Yujiao.Studies on the physiological basis of quality formation of rapeseed under different light intensity[D].Wuhan:Huazhong Agricultural University,2005.
• [3]LI H W,TONG Y P,LI B,JING R L,LU C M,LI Z S.Genetic analysis of tolerance to photo-oxidative stress induced by high light in winter wheat(Triticum aestivum L.)[J].Journal of Genetics and Genomics,2010,37(6):399-412.
• [4]陈坤梅,李宏伟,林凡云,陈耀锋,李滨,郑琪,李振声.利用病毒介导基因沉默方法研究小麦抗光氧化相关基因[J].作物学报,2014,40(11):1905-1913.CHEN Kunmei,LI Hongwei,LIN Fanyun,CHEN Yaofeng,LI Bin,ZHENG Qi,LI Zhensheng.Functional analysis of photo-oxidative stress responsive genes in wheat using virus-induced gene silencing system[J].Acta Agronomica Sinica,2014,40(11):1905-1913.
• [5]赖东.水稻光氧化基因LPO1(t)的初步定位[D].南京:南京农业大学,2012.LAI Dong.Genetic mapping of a leaf photooxidation gene,LPO1(t)in rice(Oryza sativa L.)[D].Nanjing:Nanjing Agricultural University,2012.
• [6]刘鑫磊,满为群,杜维广,栾晓燕,陈怡,杨玉芳.大豆品种(系)间耐光氧化的差异及其与产量的关系[J].大豆科学,2005,24(4):296-300.LIU Xinlei,MAN Weiqun,DU Weiguang,LUAN Xiaoyan,CHEN Yi,YANG Yufang.Difference of photosynthetic tolerance to photooxidation among soybean varieties(lines)and relationship between it and yield[J].Soybean Science,2005,24(4):296-300
• [7]黄俊,吴震,郭世荣.白菜耐光氧化能力鉴定及其与活性氧代谢关系的研究[J].园艺学报,2006,33(5):979-984.HUANG Jun,WU Zhen,GUO Shirong.Identification of photooxidation tolerance and its relationship with metabolism of reactive oxygen species in Pak-choi[J].Acta Horticulturae Sinica,2006,33(5):979-984.
• [8]魏海蓉,宗晓娟,谭钺,王甲威,张道辉,陈新,徐丽,刘庆忠.温室弱光条件下樱桃砧木幼苗移栽到自然光下的光合和荧光特性研究[J].果树学报,2014,31(增刊):78-83.WEI Hairong,ZONG Xiaojuan,TAN Yue,WANG Jiawei,ZHANG Daohui,CHEN Xin,XU Li,LIU Qingzhong.Studies on characteristics of photosynthesis and chlorophyll fluorescence of sweet cherry rootstock seedlings transplanted from lowlightgrown in greenhouse to sunlight[J].Journal of Fruit Science,2014,31(suppl.):78-83.
• [9]徐凯.草莓的光抑制特性及光质对其生长结果的影响[D].杭州:浙江大学,2005.XU Kai.Photoinhibition of photosynthesis in strawberry and effects of light quality on its growth and fruiting[D].Hangzhou:Zhejiang University,2005.
• [10]杨兴洪,邹琦,王玮.遮阴棉花转入强光后光合作用的光抑制及其恢复[J].植物学报,2001,43(2):1255-1259.YANG Xinghong,ZOU Qi,WANG Wei.Photoinhibition in shaded cotton leaves after exposing to high light and the time course of its restoration[J].Acta Botanica Sinica,2001,43(12):1255-1259.
• [11]杨丹霞,张亚田,蔡敦江,孙广玉.育苗温室弱光下的玉米幼苗移栽到自然光下的光合和荧光特性研究[J].玉米科学,2010,18(5):60-64.YANG Danxia,ZHANG Yatian,CAI Dunjiang,SUN Guangyu.Studies on photosynthetic characteristics and chlorophyll fluorescence in maize seedlings transferred from lowlight-grown in greenhouse to sunlight[J].Journal of Maize Sciences,2010,18(5):60-64.
• [12]王帅,王海波,王孝娣,史祥宾,王宝亮,郑晓翠,刘凤之.施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响[J].果树学报,2015,32(2):206-214.WANG Shuai,WANG Haibo,WANG Xiaodi,SHI Xiangbin,WANG Baoliang,ZHENG Xiaocui,LIU Fengzhi.Effects of selenium and 6-BA on leaf senescence and active oxygen metabolism in grape[J].Journal of Fruit Science,2015,32(2):206-214.
• [13]TAN W,MENG Q W,BRESTIC M,OLSOVSKA K,YANG X H.Photosynthesis is improved by exogenous calcium in heatstressed tobacco plants[J].Journal of Plant Physiology,2011,168(17):2063-2071.
• [14]张睿佳,李瑛,虞秀明,娄玉穗,许文平,张才喜,赵丽萍,王世平.高温胁迫与外源油菜素内酯对‘巨峰’葡萄叶片光合生理和果实品质的影响[J].果树学报,2015,32(4):590-596.ZHANG Ruijia,LI Ying,YU Xiuming,LOU Yusui,XU Wenping,ZHANG Caixi,ZHAO Liping,WANG Shiping.Effects of heat stress and exogenous brassinolide on photosynthesis of leaves and berry quality of‘Kyoho’grapevine[J].Journal of Fruit Science,2015,32(4):590-596.
• [15]郑秋玲.温度胁迫对葡萄生长的影响及叶面肥喷布效应[D].泰安:山东农业大学,2010.ZHENG Qiuling.Temperature stress on grape growth and effects of foliar fertilizer spraying[J].Tai’an:Shandong Agricultural University,2010.
• [16]谭伟,梁婷,翟衡.乙草胺对葡萄叶片光合和叶绿素荧光特性及叶绿体结构的影响[J].应用生态学报,2012,23(8):2185-2190.TAN Wei,LIANG Ting,ZHAI Heng.Effects of acetochlor on the photosynthetic and fluorescence characteristics and chloroplast structure of grape leaves[J].Chinese Journal of Applied Ecology,2012,23(8):2185-2190.
• [17]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:165-260.LI Hesheng.Principles and techniques of plant physiology and biochemistry experiment[M].Beijing:Higher Education Press,2006:165-260.
• [18]杨志晓,丁燕芳,张小全,薛刚,王轶,任学良,任周营,杨铁钊.赤星病胁迫对不同抗性烟草品种光合作用和叶绿素荧光特性的影响[J].生态学报,2015,35(12):4146-4154.YANG Zhixiao,DING Yanfang,ZHANG Xiaoquan,XUE Gang,WANG Yi,REN Xueliang,REN Zhouying,YANG Tiezhao.Impacts of Alternaria alternata stress on characteristics of photosynthesis and chlorophyll fluorescence in two tobacco cultivars with different resistances[J].Acta Ecologica Sinica,2015,35(12):4146-4154.
• [19]赵滢,艾军,王振兴,秦红艳,张庆田,许培磊,刘迎雪,沈育杰.外源NO对Na Cl胁迫下山葡萄叶片叶绿素荧光和抗氧化酶活性的影响[J].核农学报,2013,27(6):867-872.ZHAO Ying,AI Jun,WANG Zhenxing,QIN Hongyan,ZHANG Qingtian,XU Peilei,LIU Yingxue,SHEN Yujie.Effects of exogenous nitric oxide on chlorophyll fluorescence and antioxidant enzymes activity in Amur grape(Vitis amurensis Rupr)leaves under salt stress[J].Journal of Nuclear Agricultural Sciences,2013,27(6):867-872.
• [20]蔡永萍,李玲,李合生,骆炳山,林毅.霍山石斛叶片光合速率和叶绿素荧光参数的日变化[J].园艺学报,2004,31(6):778-783.CAI Yongping,LI Ling,LI Hesheng,LUO Bingshan,LIN Yi.Daily change of photosynthesis and chlorophyll fluorescence of Dendrobium huoshanense[J].Acta Horticulturae Sinica,2004,31(6):778-783.
• [21]王晨光,郝兴宇,李红英,韩渊怀,韩雪,宗毓铮,李萍.CO2浓度升高对大豆光合作用和叶绿素荧光的影响[J].核农学报,2015,29(8):1583-1588.WANG Chenguang,HAO Xingyu,LI Hongying,HAN Yuanhuai,HAN Xue,ZONG Yuzheng,LI Ping.Effects of elevated atmospheric CO2concentration on soybean photosynthesis and chlorophyll fluorescence parameters[J].Journal of Nuclear Agricultural Sciences,2015,29(8):1583-1588.
• [22]尚宏芹,高昌勇.外源NO对高温胁迫下辣椒幼苗生长和生理指标的影响[J].核农学报,2015,29(8):1617-1623.SHANG Hongqin,GAO Changyong.Effects of exogenous nitric oxide on growth and physiological indexes of pepper seedlings under high temperature stress[J].Journal of Nuclear Agricultural Sciences,2015,29(8):1617-1623.
• [23]刘昭伟,张盼,王瑞,蒯婕,李磊,王友华,周治国.花铃期土壤持续干旱对棉铃对位叶气体交换参数和叶绿素荧光特性的影响[J].应用生态学报,2014,25(12):3533-3539.LIU Zhaowei,ZHANG Pan,WANG Rui,KUAI Jie,LI Lei,WANG Youhua,ZHOU Zhiguo.Effects of soil progressive drought during the flowering and boll-forming stage on gas exchange parameters and chlorophyll fluorescence characteristics of the subtending leaf to cotton boll[J].Chinese Journal of Applied Ecology,2014,25(12):3533-3539.
• [24]徐凯.草莓的光抑制特性及光质对其生长结果的影响[D].杭州:浙江大学,2005.XU Kai.Photoinhibition of photosynthesis in strawberry and effect of light quality on its growth and fruiting[D].Hangzhou:Zhejiang University,2005.
• [25]WANG P,DUAN W,TAKABAYASHI A,ENDO T,SHIKANAI T,YE J Y,MI H L.Chloroplastic NAD(P)H dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress[J].Plant Physiology,2006,141(2):465-474.
• [26]李涛,丁在松,关东明,陈传永,孙锐,赵明.水稻远缘杂交后代的耐强光和抗光氧化特性[J].作物学报,2006,32(12):1913-1916.LI Tao,DING Zaisong,GUAN Dongming,CHEN Chuanyong,SUN Rui,ZHAO Ming.Characteristics of photosynthesis and photo-oxidation in the hybrid between Oryza sativa and Oryza rufipogon[J].Acta Agronomica Sinica,2006,32(12):1913-1916.
• [27]闫宁,王晓清,王志丹,张艳丽,薛惠民,郭得平.食用黑粉菌侵染对茭白植株抗氧化系统和叶绿素荧光的影响[J].生态学报,2013,33(5):1584-1593.YAN Ning,WANG Xiaoqing,WANG Zhidan,ZHANG Yanli,XUE Huimin,GUO Deping.Antioxidative system and chlorophyll fluorescence of Zizania latifolia Turcz.plants are affected by Ustilago esculenta infection[J].Acta Ecologica Sinica,2013,33(5):1584-1593.
• [28]孙锦,贾永霞,郭世荣,李娟.海水胁迫对菠菜(Spinacia olerancea L.)叶绿体活性氧和叶绿素代谢的影响[J].生态学报,2009,29(8):4361-4371.SUN Jin,JIA Yongxia,GUO Shirong,LI Juan.Effects of sea water stress on metabolism of reactive oxygen species and chlorophyll in chloroplasts of spinach(Spinacia olerancea L.)[J].Acta Ecologica Sinica,2009,29(8):4361-4371.
• [29]LIANG Y C,HU F,YANG M C,YU J.Antioxidative defenses and water deficit induced oxidative damage in rice(Oryza sativa L.)growing on non-flooded paddy soils with ground mulching[J].Plant and Soil,2003,257(2):407-416.
• [30]车俊峰,郭春会,苏婷.叶面肥组配喷施对克瑞森无核葡萄产量和品质的影响[J].干旱地区农业研究,2011,29(3):174-179.CHE Junfeng,GUO Chunhui,SU Ting.Effect of different combination of foliar fertilizers on the yield and quality of Crimson seedless grape[J].Agricultural Research in the Arid Areas,2011,29(3):174-179.
• [31]ARAUJO W L,NUNES-NESI A,NIKOLOSKI Z,LEE SWEETLOVE J,FERNIE A R.Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues[J].Plant,Cell&Environment,2012,35(1):1-21.
• [32]FEDOSEEVA I V,VARAKINA N N,RUSALEVA T M,BOROVSKII G B,RIKHVANOV E G,VOINIKOV V K.Effect of calcium ions on the Hsp104 synthesis and heat tolerance of Saccharomyces cerevisiae[J].Microbiology,2010,79(2):153-159.
• [33]YANG S,WANG F,GUO F,MENG J J,LI X G,WAN S B.Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance[J].Journal of Integrative Plant Biology,2015,57(5):486-495.
• [34]孙克香,杨莎,郭峰,刘翠敏,孟静静,胡春梅,李新国.高温强光胁迫下外源钙对甜椒(Capsicum fructescens L.)幼苗光合生理特性的影响[J].植物生理学报,2015,51(3):280-286.SUN Kexiang,YANG Sha,GUO Feng,LIU Cuimin,MENG Jingjing,HU Chunmei,LI Xinguo.Effects of exogenous calcium on photosynthetic chracteristics of sweet pepper(Capsicum fructescens L.)seedlings[J].Plant Physiology Journal,2015,51(3):280-286.
• [35]王芳,杨莎,郭峰,孟静静,孟庆伟,万书波,李新国.钙对花生幼苗生长、活性氧积累和光抑制程度的影响[J].生态学报,2015,35(5):1496-1504.WANG Fang,YANG Sha,GUO Feng,MENG Jingjing,MENG Qingwei,WAN Shubo,LI Xinguo.Effects of calcium on peanut(Arachis hypogaea L.)seedling growth,accumulation of reactive oxygen species and photoinhibition[J].Acta Ecologica Sinica,2015,35(5):1496-1504.