陈能阜;赵光武;钟泰林;

• 1:浙江省农业项目服务中心
• 2:浙江农林大学农业与食品科学学院
• 3:浙江农林大学暨阳学院

摘要(Abstract)：

【目的】探讨氧传感技术在板栗种子活力快速测定中的应用效果。【方法】分别采集板栗‘浙早1号’‘浙早2号’各5个母株的种子材料,通过老化处理0、24、48 h,分别获得各品种3个活力梯度的15份种子样品,然后进行氧传感测定以及室内标准发芽和田间出苗比较试验。【结果】经老化处理后,板栗种子活力开始下降,老化时间越长下降幅度越大。相关分析表明,氧气消耗指数(O_(CI))、氧气消耗速率(OCR)与‘浙早1号’板栗种子的标准发芽率、杭州及温州田间出苗率均呈显著相关,O_(CI)、理论萌发时间(TGT)与‘浙早2号’板栗种子的3个发芽指标均呈显著相关。【结论】O_(CI)是评价2个板栗品种种子活力的最佳指标,并在贮藏种子的活力检测中得到了验证。

关键词(KeyWords)： 板栗;种子活力;氧传感技术;氧气消耗指数(OCI)

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业(农业)科研专项(201303002);; 国家自然科学基金(30800890);; 浙江省高校教师专业发展项目(FX2015050)

作者(Authors): 陈能阜;赵光武;钟泰林;

DOI: 10.13925/j.cnki.gsxb.20170231

参考文献(References)：

• [1]桑红梅,彭祚登,李吉跃.我国林木种子活力研究进展[J].种子,2006,25(6):55-59.SANG Hongmei,PENG Zuodeng,LI Jiyue.Research progress on seed vigor of forest trees in China[J].Seed,2006,25(6):55-59.
• [2]SKOUSEN J,COOK T,WILSON-KOKES L,PENA-YEWTUKHIW E.Survival and growth of chestnut backcross seeds and seedlings on surface mines[J].Journal of Environmental Quality,2013,42(3):690-695.
• [3]刘明久,王铁固,陈士林,王春虎,赵新亮.玉米种子人工老化过程中生理特性与种子活力的变化[J].核农学报,2008,22(4):510-513.LIU Mingjiu,WANG Tiegu,CHEN Shilin,WANG Chunhu,ZHAO Xinliang.Physioloycial and seed vigour changes of maize seeds during artificial aging course[J].Journal of Nuclear Agricultural Sciences,2008,22(4):510-513.
• [4]ZHONG T L,ZHAO G W.Application of oxygen-sensing technology to measure seed quality of Ginkgo biloba[J].Journal of Forestry Research,2017,28(4):725-731.
• [5]LI H Y,WANG Y X,WANG C,ZHANG S H,LI S H,ZHOU G Q,WANG S X,ZHANG J C.Extraction,selenylation modification and antitumor activity of the glucan from Castanea mollissima Blume[J].Glycoconjugate Journal,2017,34(2):207-217.
• [6]刘国彬,兰彦平,曹均,周连第,兰卫宗.板栗雄花败育资源及其利用研究[J].果树学报,2013,30(4):582-585.LIU Guobin,LAN Yanping,CAO Jun,ZHOU Liandi,LAN Weizong.Research and utilization of male-inflorescence-abortive resource in Castanea mollissima[J].Journal of Fruit Science,2013,30(4):582-585.
• [7]邱文明,何秀娟,徐育海.板栗花芽性别调控研究进展[J].果树学报,2015,32(1):142-149.QIU Wenming,HE Xiujuan,XU Yuhai.Research progress on sex control of chestnut flower buds[J].Journal of Fruit Science,2015,32(1):142-149.
• [8]刘畅,王书军,王硕.板栗淀粉结构和功能特性研究进展[J].中国粮油学报,2016,31(11):157-162.LIU Chang,WANG Shujun,WANG Shuo.Structure and functional properties of chestnut starches[J].Journal of the Chinese Cereals and Oils Association,2016,31(11):157-162.
• [9]LICHATSCHOV B S.The importance of determining vigor in the evaluation of seed quality[J].Seed Science and Technology,1981,9:623-631.
• [10]JR MCDONALD M B.Vigor test subcommittee report[J].AOSA Newsletter,1980,54:37-40.
• [11]HOFFMASTER A L,FUJIMURA K,MCDONALD M B.An automated system for vigor testing three-day-old soybean seedlings[J].Seed Science and Technology,2003,31(3):701-713.
• [12]PANDEY P K,GOYAL R D,PRAKASH V,KATIYAR R P,SINGH C B.Association between laboratory vigour tests and field emergence in cucurbits[J].Seed Research,1990,18:40-43.
• [13]SHEN T Y,ODEN P C.Fumarase activity as a quick vigour test for Scots pine(Pinus sylvestris L.)seeds[J].Seed Science and Technology,2000,28(3):825-835.
• [14]TEKRONY D M.Precision is an essential component in seed vigour testing[J].Seed Science and Technology,2003,31(2):435-447.
• [15]WANG Y R,YU L,NAN Z B.Vigor tests used to rank seed lot quality and predict field emergence in four forage species[J].Crop Science,2004,44(2):535-541.
• [16]ZHAO G W,SUN Q,WANG J H.Improving seed vigour assessment of super sweet corn and sugar enhanced sweet corn[J].New Zealand Journal of Crop and Horticultural Science,2007,35(3):349-356.
• [17]赵光武,许芳忠,钟泰林.基于氧传感技术测定杉木种子活力的初步研究[J].种子,2011,30(4):4-7.ZHAO Guangwu,XU Fangzhong,ZHONG Tailin.Preliminary study on seed vigor testing of China fir based on oxygen sensing technology[J].Seed,2011,30(4):4-7.
• [18]陶嘉龄,郑光华.种子活力[M].北京:科学出版社,1991.TAO Jialing,ZHENG Guanghua.Seed vigor[M].Beijing:Science Press,1991.
• [19]赵光武,钟泰林,应叶青.现代种子种苗实验指南[M].北京:中国农业出版社,2015.ZHAO Guangwu,ZHONG Tailin,YING Yeqing.Modern seed and seedling experiment guide[M].Beijing:China Agriculture Press,2015.
• [20]WANG B S P.Laboratory germination criteria for red pine(Pinus resinosa Ait.)seed[J].Proceedings of the Association of Official Seed Analysts,1973,63:94-101.
• [21]BONNER F T.Measurement of seed vigor for loblolly(Pinus taeda)and slash pines(Pinus elliottii)[J].Forest Science,1986,32(3):170-178.
• [22]郑林.林木种子活力的电导法测定[J].福建林业科技,1996,23(4):11-14.ZHENG Lin.The determination of forest tree seed vigor by a conductance method[J].Journal of Fujian Forestry Science&Technology,1996,23(4):11-14.
• [23]ZHAO G W,ZHONG T L.Improving the assessment method of seed vigor in Cunninghamia lanceolata and Pinus massoniana based on oxygen sensing technology[J].Journal of Forestry Research,2012,23(1):95-101.
• [24]PERRY D A.Report of the vigor test committee 1974-1977[J].Seed Science and Technology,1978,6:159-181.
• [25]PERL M,KRETSCHMER M.Biochemical activities and compounds in seeds:Possible tools for seed quality evaluation[J].Annals of Botany,1988,62(1):61-68.
• [26]李淑娴,陈幼生,吴琼美.湿地松种子活力测定方法的研究[J].南京林业大学学报,1996,20(3):16-19.LI Shuxian,CHEN Yousheng,WU Qiongmei.A study on seed vigor index of slash pine[J].Journal of Nanjing Forestry University,1996,20(3):16-19.