水分胁迫下柑桔果皮的生化变化与复水后补偿生长的关系Biochemical Changes in the Rind of Water Stressed Citrus Fruit as Related to Compensatory Growth After Rewatering
黄旭明,黄辉白,吕雪娟
摘要(Abstract)：
比较了控水与正常浇水处理的盆栽柑桔（CitrusreticulataBlanco.ev.Zhuju）果实的果皮中过氧化物酶（POD）、SDS-不可溶性（共价键壁结合）蛋白氨基酸组成、羟脯氨酸（Hpro）含量及SDS-可溶性成分中氨基酸组成与含量。结果表明，柑桔砂囊膨大早期，水分胁迫并未引起果皮中POD活性提高及共价键壁结合结构蛋白在质与量上的明显改变，显示POD介导的细胞壁硬化未被激活，而氨基酸组成中最明显的变化是脯氨酸（Pro）比率大幅提高，这暗示渗透调节已经启动，这些均有利于胁迫果复水后迅速恢复膨大生长。
关键词(KeyWords)： 柑桔;水分胁迫;过氧化物酶;氨基酸组成;羟脯氨酸;脯氨酸
基金项目(Foundation): 国家自然科学基金
作者(Author): 黄旭明,黄辉白,吕雪娟
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参考文献(References)：
- 1黄辉白,高飞飞,许建楷,等.水分胁迫对甜橙果实发育的影响.园艺学报,1986,13(4):238～244
- 2黄辉白,高飞飞,李建国.果实膨大生长和吸水与气候变化之间的关系园艺学报,1994,21(2):124～128
- 3Li SH, Huguet JG.Scholch PG, et al. Response of peach tree growth and cropping to soil water deficit at various pheno-logical stages of fruit development.J Hort Sci,1989,64,541～542
- 4李绍华.果树生长发育、产量和果树品质对水分胁迫反应的敏感期及节水灌溉植物生理学通讯,1993,29(1):10～16
- 5Chalmers DJ, Mitchell PD, Heek LV. Control of peach of tree glowth and productivity by regulated water supply, treedensity and summer pruning.J Amer Soc Hort Sci,1981,106:307～312
- irrigstion.Aust J Plant Physiol,1988,15:633～639
- 6Cohen A ,Goell A. Fruit growth and dry matter accumulation in grape-fruit during period of water withholding and after irrigation .Aust J Plant Physiol ,1988, 15: 633～639
- 7 Kramer PJ.Water Relations of Plant.New York:Academic Press,1983.357
- 8李连潮,王学臣.水分亏缺对植物细胞壁的影响及其与细胞壁延伸生长的关系植物生理学通讯,1996,32:321～327
- 9 Lamport DTA.Carbohydrates:Structure and function.In,Preiss J(ed.)The biochemistry of plant.Vol.3.New York.Academic Press,1980.501～541
- 10黄海,沈曾佑,张志良,等.细胞壁中富含羟脯氨酸蛋白质与烟草细胞扩大生长的关系.植物生理学报,1983,9(1):85～91
- 11张志良.植物生理学实验指导.北京:高等教育出版社,1990.154
- 12汤章城.植物对渗透和淹水胁迫的适应机理.见:余叔文主编.植物生理与分子生物学北京:科学出版杜,1992.404～416
- 13 Nuemann PM. The role of wall adjustment in plant resistance to water deficits. Crop Sol, 1995, 35: 1258～1266