‘富有’柿果实韧皮部胞间连丝研究Ultrastructural investigation on plasmodesmata in phloems in developing fruits of persimmon ‘Fuyu'
刘林;
摘要(Abstract):
【目的】果实韧皮部胞间连丝分布特点决定韧皮部同化物卸载途径类型,为了解‘富有’柿果实膨大期韧皮部胞间连丝分布特点,【方法】采用透射电子显微技术对膨大期果实韧皮部做超微结构研究,统计不同类型细胞界面上胞间连丝发生频率。【结果】结果表明,柿果实维管束没有维管束鞘,直接与果肉细胞连接,韧皮部由筛管分子、伴胞和维管薄壁细胞构成,筛管-伴胞界面、伴胞-维管薄壁细胞界面、维管薄壁细胞-维管薄壁细胞界面以及维管薄壁细胞-果肉细胞界面上都存在胞间连丝,伴胞-维管薄壁细胞界面上的胞间连丝发生频率约为维管薄壁细胞-果肉细胞界面上的一半。【结论】据此认为,‘富有’柿果实韧皮部伴胞与维管薄壁细胞界面上分布有中等数量的胞间连丝,显示韧皮部卸载途径为共质体类型。
关键词(KeyWords): ‘富有’柿;果实;韧皮部;胞间连丝;超微结构
基金项目(Foundation): 临沂大学科研基金(HX09104);; 临沂市科技局计划项目(200101);; 山东省教育厅科研计划项目(J01H51);; 山东省自然科学基金(ZR2011CL002)
作者(Authors): 刘林;
DOI: 10.13925/j.cnki.gsxb.2012.05.030
参考文献(References):
- [1]AYRE B G.Membrane-transport systems for sucrose in relation towhole-plant carbon partitioning[J].Mol Plant,2011,4(3):377-394.
- [2]GEIGER D.Plant sucrose transporters from a biophysical point ofview[J].Mol Plant,2011,4(3):395-406.
- [3]PATRICK J W.Phloem unloading:sieve element unloading and post-sieve element transport[J].Annu Rev Plant Physiol Plant Mol Biol,1997,48:191-222.
- [4]HU L,SUN H,LI R,ZHANG L,WANG S,SUI X,ZHANG Z.Phloem unloading follows an extensive apoplasmic pathway in cu-cumber(Cucumis sativus L.)fruit from anthesis to marketable ma-turing stage[J].Pant Cell Environ,2011,34(11):1835-1848.
- [5]ZHANG L Y,PENG Y B,PELLESCHI-TRAVIER S,FAN Y,LUY F,LU Y M,GAO X P,SHEN Y Y,DELROT S,ZHANG D P.Evidence for apoplasmic phloem unloading in developing apple fruit[J].Plant Physiol,2004,135:574-586.
- [6]WU G L,ZHANG X Y,ZHANG L Y,PAN Q H,SHEN Y Y,ZHANG D P.Phloem unloading in developing walnut fruit is sym-plasmic in the seed pericarp and apoplasmic in the fleshy pericarp[J].Plant Cell Physiol,2004,45(10):1461-1470.
- [7]XIA Guo-hai,ZHANG Da-peng.Intercellular symplastic connectionand isolation of the unloading zone in flesh of the developing grapeberry[J].Acta Botanica Sinica,2000,42(9):898-904.夏国海,张大鹏.葡萄果肉同化物卸载区细胞间的共质体联系与隔离[J].植物学报,2000,42(9):898-904.
- [8]ZHANG X Y,WANG X L,WANG X F,XIA G H,PAN Q H,FANR C,WU F Q,YU X C,ZHANG D P.A shift of phloem unloadingfrom symplasmic to apoplasmic pathway is involved in developmentalonset of ripening in grape berry[J].Plant Physiol,2006,142:220-232.
- [9]ROBERTS A G,CRUZ S S,ROBERTS I M,PRIOR D A M,TUR-GEON R,OPARKA K J.Phloem unloading in sink leaves of Nico-tiana benthamíana:comparison of a fluorescent solute with a fluores-cent virus[J].Plant Cell,1997,9:1381-1396.
- [10]VIOLA R,ROBERTS A G,HAUPT S,GAZZANI S,HANCOK RD,MARMIROLI N,MACHRAY G C,OPARKA K J.Tuberizationin potato involves a switch from apoplastic to symplastic phloem un-loading[J].Plant Cell,2001,13:385-398.
- [11]LI Jing,NIE Ji-yun,LI Hai-fei,XU Guo-feng,WANG Xiao-di,WU Yong-long,WANG Zhen-xu.On determination conditions fortotal polyphenols in fruits and its derived products by Folin-phenolmethods[J].Journal of Fruit Science,2008,25(1):126-131.李静,聂继云,李海飞,徐国峰,王孝娣,毋永龙,王贞旭.Folin-酚法测定水果及其制品中总多酚含量的条件[J].果树学报,2008,25(1):126-131.
- [12]JANG I C,JO E K,BAE M S,LEE H J,JEON G I,PARK E,YUKH G,AHN G H,LEE S C.Antioxidant and antigenotoxic activitiesof different parts of persimmon(Diospyros kaki cv.Fuyu)fruit[J].Journal of Medicinal Plants Research,2010,4(2):155-160.
- [13]FISHER D G.Plasmodesmatal frequency and other structural aspectsof assimilate collection and phloem loading in leaves of Sonchus ol-eraceus(Asleraceae),a species with minor vein transfer cells[J].Am J Bot,1991,78:1549-1559.
- [14]BOTHA C E J,HARTLEY B J,CROSS R H M.The ultrastructureand computer-enhanced digital image analysis of plasmodesmata atthe Kranz mesophyll-bundle sheath interface of Themeda triandravar.imberbis(Retz)A.camus in conventionally fixed leaf blades[J].Ann Bot,1993,72:255-261.
- [15]VERCHOT-LUBICZ J.Plasmodesmata transport of GFP and GFPfusions requires little energy and transitions during leaf expansion[J].Plant Signal Behav,2008,3(10):902-905.
- [16]LUCAS W J.Plant viral movement proteins:agents for cell-to-celltrafficking of viral genomes[J].Virology,2006,344:169-184.
- [17]LUCAS W J,LEE J Y.Plasmodesmata as a supracellular controlnetwork in plants[J].Nat Rev Mol Cell Biol,2004,5:712-726.
- [18]COOK M E,GRAHAM L E,BOTHA C E J,LAVIN C A.Compara-tive ultrastructure of plasmodesmata of Chara and selected bryophytes:toward an elucidation of the evolutionary origin of plant plasmodes-mata[J].Am J Bot,1997,84(9):1169-1178.
- [19]FAULKNER C,BRANDOM J,MAULE A,OPARKA K.Plasmod-esmata 2004.Surfing the symplasm[J].Plant Physiol,2005,137:607-610.
- [20]MEDINA E N,HAUPT S,THOW G,BOEVINK P,CHAPMAN S,OPARKA K.High-throughput viral expression of cDNA-green fluo-rescent protein fusions reveals novel subcellular addresses and iden-tifies unique proteins that interact with plasmodesmata[J].Plant Cell,2003,15:1507-1523.
- [21]NIE P,WANG X,HU L,ZHANG H,ZHANG J,ZHANG Z,ZHANGL.The predominance of the apoplasmic phloem-unloading pathwayis interrupted by a symplasmic pathway during Chinese jujube fruitdevelopment[J].Plant Cell Physiol,2010,51(6):1007-1018.
- [22]RENNIE E A,TURGEON R.A comprehensive picture of phloemloading strategies[J].PNAS,2009,106(33):14162-14167.
- [23]GAMALEI Y.Structure and function of leaf minor veins in trees andherbs[J].Trees,1989,3:96-110.