郑焕;任俊鹏;贾玥;章镇;陶建敏;

• 1:南京农业大学园艺学院

摘要(Abstract)：

‘2004-6-12’是南京农业大学从欧亚种‘魏可’自交实生后代中选育出来的雄性不育优良单株。以葡萄雄性不育新种质‘2004-6-12’及其雄性可育亲本‘魏可’为试材,采用酶联免疫(ELISA)检测技术对花药发育不同时期(1.造孢期、2.花粉母细胞期、3.减数分裂期、4.四分体期、5.单核期、6.二核期、7.成熟花粉期)的花蕾组织中的吲哚乙酸(IAA)、赤霉素(GAS)、细胞分裂素(CTK)、脱落酸(ABA)含量进行测定。结果表明,‘2004-6-12’与‘魏可’花蕾中的IAA,GAS的含量在减数分裂期之前差异不显著,在四分体期及单核期‘2004-6-12’显著低于‘魏可’。‘2004-6-12’中的CTK含量在减数分裂期之前显著低于‘魏可’。‘魏可’在单核期ABA含量开始降低,而‘2004-6-12’在二核期才开始降低。‘2004-6-12’的IAA/ABA和GAS/ABA在二核期之前明显高于‘魏可’且比‘魏可’比值变化较大,因此认为IAA,GAS,CTK的亏缺,ABA的延后降低及IAA/ABA,GAS/ABA比例失调与葡萄雄性不育有一定的关系。

关键词(KeyWords)： 葡萄;雄性不育;内源激素

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系(CARS-30);; 江苏省科技厅(BE2009314-2);; 江苏省农业科技自主创新资金(CX(10)110)

作者(Author): 郑焕;任俊鹏;贾玥;章镇;陶建敏;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.03.025

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