卢博彬;黄天林;潘丽佳;傅嘉欣;王英;刘朝吉;胡桂兵;刘成明;

• 1:华南农业大学园艺学院
• 2:广东省潮州市果树研究所
• 3:亚热带农业生物资源保护与利用国家重点实验室
• 4:华南园艺作物种质创新与利用广东省普通高校重点实验室

摘要(Abstract)：

【目的】在龙眼上,营养系杂交和杂种真实性早期鉴定的工作都还开展甚少,为了研发出既操作简便又稳定可靠的早期鉴定技术,【方法】以人工杂交获得的‘凤梨朵’ב大乌圆’的大规模F1代杂交群体(简称FD群体)中的230个单株为试材,先通过40个RAPD引物对双亲进行扩增筛选获得父本特异性的显性标记,再以父本的小规模自交群体(9株)为材料,明确这些标记在自交群体中的分离情况,最终鉴别出纯显性标记并用于对FD群体的杂种真实性鉴定。【结果】结果表明,对双亲进行扩增的RAPD引物中有9个能扩增出父本特异性的显性标记,用这9个引物检测父本自交群体的分离情况,最终鉴别出纯显性标记1个,用其对FD群体中的230个单株进行杂种真实性鉴定,发现有223个单株为真杂种,真杂种比例高达96.96%。【结论】既获得了早期鉴定杂种真实性的简便方法,又证明了创建的龙眼杂种群体非常可靠,值得在今后的育种工作中借鉴和使用。

关键词(KeyWords)： 龙眼;营养系杂交育种;杂种鉴定

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2012A020200005,2009B020202001);; 广东省现代农业产业技术体系(LNSG-2011-5,LNSG-2010-5);; 广东省国际合作项目(2009B050300004);; 国家自然科学基金(30771498,30270922);; 中国博士后基金(20090460780,20080440763)

作者(Author): 卢博彬;黄天林;潘丽佳;傅嘉欣;王英;刘朝吉;胡桂兵;刘成明;

Email:

DOI: 10.13925/j.cnki.gsxb.2013.03.010

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