刘扩展;王力荣;朱更瑞;方伟超;李勇;曹珂;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】探讨‘红根甘肃桃1号’抗南方根结线虫的生理机制。【方法】选取对南方根结线虫免疫的‘红根甘肃桃1号’、高抗的‘白根甘肃桃1号’、高感的‘贝蕾’以及‘红根甘肃桃1号’与‘贝蕾’的回交群体BC1为试验材料,采用广泛靶向代谢组技术测定样品间的代谢组成分,并利用转录组分析了黄酮醇合成途径关键基因在‘红根甘肃桃1号’与‘贝蕾’中的表达。【结果】在不同样品间,共检出17类、427种代谢产物,比较这些物质在BC1亲本和抗/感病混合池材料以及‘红根甘肃桃1号’和‘白根甘肃桃1号’种质间的含量变化,鉴定出4种差异的代谢物质,其中甲基槲皮素O-己糖苷的相对含量在‘红根甘肃桃1号’中是‘白根甘肃桃1号’的31.15倍。转录组分析表明,黄酮醇合成酶基因在不同抗性种质间表达有明显差异,其关键基因为Prupe.1G502300。【结论】差异代谢物质甲基槲皮素O-己糖苷可能作为一种重要的抗线虫物质,参与了‘红根甘肃桃1号’对南方根结线虫的免疫反应,该研究为解析‘红根甘肃桃1号’抗根结线虫的生理和分子机制奠定理论基础。

关键词(KeyWords)： ‘红根甘肃桃1号’;南方根结线虫;代谢产物;甲基槲皮素O-己糖苷;候选基因

Abstract:

Keywords:

基金项目(Foundation): 国家作物种质资源保护和利用专项(2013NWB038);; 中国农业科学院科技创新工程（CAAS-ASTIP-2019-ZFRI-01）

作者(Author): 刘扩展;王力荣;朱更瑞;方伟超;李勇;曹珂;

Email:

DOI: 10.13925/j.cnki.gsxb.20190488

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