计玮玮;邱翠花;焦云;郭延平;滕元文;

• 1:浙江大学园艺系·农业部园艺植物生长发育与生物技术重点开放实验室
• 2:西北农林科技大学园艺学院

摘要(Abstract)：

【目的】研究夏季高温强光下砂梨叶片光合特征的变化,探讨梨光抑制的分子机制。【方法】以1年生‘翠冠’和‘圆黄’梨的盆栽苗为试验材料,测定了夏季晴天高温强光对气体交换参数和光响应曲线的影响,以及D1蛋白、Deg1蛋白酶含量和PsbA mRNA表达的变化。【结果】‘翠冠’和‘圆黄’梨叶片在夏季晴天中午高温强光的双重胁迫下,净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、表观量子效率(AQY)、D1蛋白和Deg1蛋白酶含量显著下降,而PsbA mRNA积累增加。到了17:00,‘翠冠’的D1蛋白、Deg1蛋白酶含量得到恢复,Pn略有回升,而‘圆黄’未出现明显的恢复迹象。【结论】高温强光下2个梨品种的叶片均发生了光抑制,低含量的Deg1不是降解D1蛋白的主要蛋白酶。高温强光诱导的D1蛋白净含量的减少主要是由于D1蛋白合成在翻译阶段受到了抑制。从下午的光合和D1蛋白的恢复来看,‘翠冠’梨的PSII比‘圆黄’梨更抗高温。

关键词(KeyWords)： 砂梨;高温强光;D1蛋白;Deg1蛋白酶;PsbA

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业(梨)产业技术体系建设专项资金(nycytx-29)

作者(Authors): 计玮玮;邱翠花;焦云;郭延平;滕元文;

DOI: 10.13925/j.cnki.gsxb.2012.05.017

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