王延秀;朱燕芳;陈佰鸿;贾旭梅;郭爱霞;赵通;程丽;胡亚;

• 1:甘肃农业大学园艺学院

摘要(Abstract)：

【目的】探讨干旱胁迫下楸子对外源一氧化氮(nitric oxide,NO)处理的生理响应特性与作用机制,综合评价后筛选缓解干旱胁迫的最佳NO处理浓度。【方法】以10叶龄的楸子[Malus prunifolia (Willd.) Borkh.]实生苗为试材,采用盆栽控水的方法,在中度水分胁迫(即含水量为土壤最大持水量45%～55%)下,喷施不同浓度外源NO(180μmol·L~(-1)、190μmol·L~(-1)、200μmol·L~(-1)、210μmol·L~(-1)),测定净光合速率(Pn)等光合参数、过氧化氢酶(CAT)等抗氧化酶活性及以脯氨酸(Pro)、丙二醛(MDA)含量、相对电导率(REC)等指标。【结果】相比于对照,外源NO处理缓解了水分胁迫下楸子叶片中MDA含量、Pro、REC、胞间二氧化碳浓度(Ci)以及过氧化物酶(POD)活性的上升幅度,同时也减缓了Pn、气孔导度(Gs)、蒸腾速率(Tr)、超氧化物歧化酶(SOD)与CAT酶活性的下降幅度,且具有明显的浓度效应。【结论】主成分分析(PCA)结果显示,特征根大于1的2个主成分的方差贡献率达92.932%,190μmol·L~(-1)硝普钠(SNP)处理得分最高,效果最佳。适宜浓度SNP处理能够激活水分胁迫下楸子幼苗的抗氧化酶系统,并可以保护细胞膜系统,及增强细胞光合性能,从而提高其耐旱性。

关键词(KeyWords)： 楸子;水分胁迫;一氧化氮;光合;耐旱性;主成分分析

Abstract:

Keywords:

基金项目(Foundation): 甘肃省自然科学基金项目(145RJZA167);; 甘肃省教育厅基本科研业务项目(035-041051);; 甘肃省农牧厅生物技术专项项目(035-034070)

作者(Author): 王延秀;朱燕芳;陈佰鸿;贾旭梅;郭爱霞;赵通;程丽;胡亚;

Email:

DOI: 10.13925/j.cnki.gsxb.20180022

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