朱迎春;孙德玺;刘君璞;孙小武;

• 1:湖南农业大学园艺学院
• 2:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】壳寡糖能够诱导植物抵抗生物胁迫和非生物胁迫,探究NaCl胁迫下壳寡糖对耐盐性不同的西瓜幼苗生理特性的影响,以期为西瓜抗性育种和盐碱地西瓜栽培提供理论依据。【方法】以2个西瓜材料(‘HQ-2’和‘HQ-3’)为试材,研究在180 mmol·L~(-1)NaCl胁迫下,壳寡糖对耐盐性不同的西瓜幼苗地下和地上部干鲜质量、光合色素含量及SOD、CAT、POD活性和MDA含量的影响。【结果】添加1 g·L~(-1)的壳寡糖可以一定程度提高西瓜幼苗的地下和地上部干鲜质量,增加叶绿素a、叶绿素b、类胡萝卜素含量。在处理10 d后,在180 mmol·L~(-1)NaCl胁迫下和壳寡糖+180 mmol·L~(-1)NaCl处理下不耐盐材料‘HQ-2’的SOD、CAT活性和MDA含量均低于对应的耐盐材料‘HQ-3’,而不耐盐材料‘HQ-2’POD活性高于耐盐材料‘HQ-3’。【结论】添加壳寡糖对不耐盐材料在180 mmol·L~(-1)NaCl胁迫下缓解作用显著,对耐盐材料虽有促进作用,但效果不显著。

关键词(KeyWords)： 西瓜;NaCl胁迫;壳寡糖

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系(CARS-25);; 中国农业科学院基本科研业务费专项(Y2019XK16-03);中国农业科学院科技创新工程(CAAS-ASTIP-2020-ZFRI);; 国家重点研发计划(2018YFD0201310);; 郑州市重大科技专项(188PCXZX802)

作者(Author): 朱迎春;孙德玺;刘君璞;孙小武;

Email:

DOI: 10.13925/j.cnki.gsxb.20200035

参考文献(References)：

• [1]狄文伟.壳寡糖在蔬菜生产上的应用[J].北方园艺，2016(8):54-55.DI Wenwei. Application of chitooligosaccharides in vegetable production[J].Northern Horticulture, 2016(8):54-55.
• [2]张绿萍，解璞，袁启凤，王立娟.采前喷施壳聚糖对火龙果贮藏性能及品质的影响[J].贵州农业科学，2017,45(6):40-44.ZHANG Lüping, XIE Pu, YUAN Qifeng, WANG Lijuan. Effects of preharvest spraying chitosan on storage properties and quality of pitaya[J]. Guizhou Agricultural Sciences, 2017, 45(6):40-44.
• [3]刘幸海，李正名，王宝雷.具有农业生物活性壳寡糖的研究进展[J].农药学学报，2006,8(1):1-7.LIU Xinghai, LI Zhengming, WANG Baolei. Progress in pesticidal bioactivities for chitosan oligosaccharide[J]. Journal of Pesticide Science, 2006, 8(1):1-7.
• [4]郭卫华，赵小明，杜昱光.壳寡糖对黄瓜种子萌发和幼苗生长及光合特性的影响[J].中国农学通报，2009,25(3):164-169.GUO Weihua, ZHAO Xiaoming, DU Yuguang. Effects of oligochitosan on the seed germination seedling growth and photosynthetic characters of cucumber[J]. Chinese Agricultural Science Bulletin, 2009, 25(3):164-169.
• [5]李艳，魏立君，王青，李洪艳，赵小明，侯和胜，杜昱光.壳寡糖对油菜叶片光合特性的影响[J].中国农学通报，2010,26(2):132-136.LI Yan, WEI Lijun, WANG Qing, LI Hongyan, ZHAO Xiaoming, HOU Hesheng, Du Yuguang. Effects of oligochitosan on photosynthetic parameter of Brassica napus L. leaves[J]. Chinese Agricultural Science Bulletin, 2010, 26(2):132-136.
• [6]丁振中，曾哲灵，龚劲松，张超，雷鹏，高小燕，徐俊山，张家宏.NaCl胁迫下壳寡糖对油菜苗生长的影响[J].生物化工，2018,4(3):29-33.DING Zhenzhong, ZENG Zheling, GONG Jinsong, ZhANG Chao, LEI Peng, GAO Xiaoyan, XU Junshan, ZHANG Jiahong.Effects of chitooligosaccharides on the growth of rape seedlings stressed with NaCl[J]. Biological Chemical Engineering, 2018, 4(3):29-33.
• [7]曲丹阳，顾万荣，张立国，陈喜昌，李晶，李彩凤，魏湜.壳聚糖对镉胁迫下玉米幼苗根系抗氧化酶活性和内源激素水平的影响[J].西北植物学报，2017,37(4):719-727.QU Danyang, GU Wanrong, ZHANG Liguo, CHEN Xichang,LI Jing, LI Caifeng, WEI Shi. Effect of exognous chitosan on antioxidant enzyme activities and endogenous hormones in maize seedling roots under cadmium stress[J]. Acta Botanica BorealiOccidentalia Sinica, 2017, 37(4):719-727.
• [8]李合生.植物生理生化实验原理和技术[M].北京：高等教育出版社，2000:134-137.LI Hesheng. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing:Higher Education Press, 2000:134-137.
• [9]陈建勋，王晓峰.植物生理学实验指导[M].广州：华南理工大学出版社，2002:119-121.CHEN Jianxun, WANG Xiaofeng. Experimental guidance of plant physiology[M]. Guangzhou:South China University of Technology Press, 2002:119-121.
• [10]戴伟民，蔡润，潘俊松，何欢乐.盐胁迫对番茄幼苗生长发育的影响[J].上海农业学报，2002,18(1):58-62.DAI Weimin, CAI Run, PAN Junsong, HE Huanle. Effects of salt stress on the growth and development of tomato seedlings[J]. Acta Agricultural Shanghai, 2002, 18(1):58-62.
• [11]杨秀玲，郁继华，李雅佳，李建建，张韵.NaCl胁迫对黄瓜种子萌发及幼苗生长的影响[J].甘肃农业大学学报，2004,39(1):6-9.YANG Xiuling, YU Jihua, LI Yajia, LI Jianjian, ZHANG Yun.Effects of NaCl stress on seed germination and seedling growth of cucumis sativus[J]. Journal of Gansu Agricultural University,2004, 39(1):6-9.
• [12]韩志平，郭世荣，王其传.盐胁迫对小型西瓜幼苗生长的影响[J].北方园艺，2016(7):13-16.HAN Zhiping, GUO Shirong, WANG Qichuan. Effect of salinity growth of mini-watermelon seedings[J]. Northern Horticulture, 2016(7):13-16.
• [13]杨升，张华新，张丽.植物耐盐生理生化指标及耐盐植物筛选综述[J].西北林学院学报，2010,25(3):59-65.YANG Sheng, ZHANG Huaxin, ZHANG Li. Physiological and biochemical indices of salt tolerance and scanning of salt-tolerance plants:a review[J]. Journal of Northwest Forestry University, 2010, 25(3):59-65.
• [14]付晴晴，谭雅中，翟衡，杜远鹏.NaCl胁迫对耐盐性不同葡萄株系叶片活性氧代谢及清除系统的影响[J].园艺学报，2018,45(1):30-40.FU Qingqing, TAN Yazhong, ZHAI Heng, DU Yuanpeng. Effects of salt stress on the generation and scavenging of reactive oxygen species in leaves of grape strains with different salt tolerance[J]. Acta Agricultural Sinica, 2018, 45(1):30-40.
• [15]程丽萍，刘晋秀，胡青平.外源NO对盐胁迫下小麦幼苗叶片丙二醛、叶绿素及氧化酶的影响[J].麦类作物学报，2013,33(6):1222-1225.CHENG Liping, LIU Jinxiu, HU Qingping. Effect exogenous nitric oxide on the contents of MDA and chlorophyll and the activities of oxidases in leaves of wheat seedlings under salt[J]. Journal of Triticeae Crops, 2013, 33(6):1222-1225.
• [16] HERNNDEZ J A, FRRER M A, JIMENEZ A, BARCELO A R,SEVILLA F. Antioxidant systems and O2.-/H2O2production in the apoplast of pea leaves. its relation with salt-induced necrotic lesions in minor veins[J]. Plant Physiology, 2001, 127(3):817-831.
• [17] SAIRAM R K, SRIVASTAVA G C. Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress[J]. Plant Science,2002, 162(6):897-904.
• [18] SALMAN S R, ABOU-HUSSEIN S D, ABDEL-MAWGOUD A M R, EL-NEMR M A. Fruit yield and quality of watermelon as affected by hybrids and humic acid application[J]. Journal of Applied Sciences Research, 2005, 1(1):51-58.
• [19] PARIDA A K, DAS A B. Salt tolerance and salinity effects on plants:a review[J]. Ecotoxicology and Environmental Safety,2005, 60(3):324-349.
• [20]焦彦生,郭世荣,李娟,樊怀福,胡晓辉,王素平.钙对根际低氧胁迫下黄瓜幼苗活性氧代谢的影响[J]．西北植物学报，2006, 26(10):2056-2062.JIAO Yansheng, GUO Shirong, LI Juan, FAN Huaifu, HU Xiaohui, WANG Suping. Effect of low rhizospere oxygen stress on reactive oxygen species metabolism in cucumber seedlings[J].Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(10):2056-2062.
• [21]樊怀福,李娟,郭世荣,杜长霞.外源NO对NaCl胁迫下黄瓜幼苗生长和根系谷胱甘肽抗氧化酶系统的影响[J]．西北植物学报, 2007, 27(8):1611-1618.FAN Huaifu, LI Juan, GUO Shirong, DU Changxia. Effects of exogenous nitric oxide on growth and glutathione dependent antioxidative system in cucumber seedling roots under NaCl stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(8):1611-1618.
• [22]邹平.特定乙酰度壳寡糖诱导小麦抗盐作用及其机理研究[D].青岛：中国科学院研究生院(海洋研究所)，2015.ZOU Ping. Effect and its mechanism of oligochitosan with certain degrees of acetylation on wheat under salt stress[D]. Qingdao:The Institute of Oceanology, Chinese Academy of Sciences,2015.
• [23] MELONI D A, OLIVA M A, MARTINEZ C A, CAMBRAIA J.Photosynthesis and ability of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress[J]. Environmental and Experimental Botany, 2003, 49(1):69-76.
• [24] DUAN J J, LI J, GUO S R, KANG Y Y. Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance[J]. Journal of Plant Physiology, 2008, 165(15):1620-1635.
• [25] HOQUE M A, BANU M N A, NAKAMURA Y, SHIMOISHI Y,MURATA Y. Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells[J]. Journal of Plant Physiology, 2007, 165(8):813-824.
• [26]孙琳琳，辛士超，强晓晶，程宪国.非生物胁迫下植物水通道蛋白的应答与调控[J].植物营养与肥料学报，2015,21(4):1040-1048.SUN Linlin, XIN Shichao, QIANG Xiaojing, CHENG Xianguo.Responsive regulation of aquaporins in the plants exposed to abiotic stresses[J]. Journal of Plant Nutrition and Fertilizer, 2015,21(4):1040-1048.
• [27]刘梅，郑青松，刘兆普，郭世伟.盐胁迫下氮素形态对油菜和水稻幼苗离子运输和分布的影响[J].植物营养与肥料学报，2015,21(1):181-189.LIU Mei, ZHENG Qingsong, LIU Zhaopu, GUO Shiwei. Effects of nitrogen forms on transport and accumulation of ions in canola(B. napus L.)and rice(Oryza sativa L.)under saline stress[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(1):181-189.