柳凤;欧雄常;詹儒林;

• 1:中国热带农业科学院南亚热带作物研究所·农业部热带果树生物学重点实验室

摘要(Abstract)：

【目的】由柑橘黄单胞菌杧果致病变种引起的杧果细菌性角斑病是杧果生产上的重要病害,解析杧果细菌性角斑病病原菌(柑橘黄单胞菌杧果致病变种)XC01菌株的基因组序列信息,为深入研究病菌的致病机制提供序列背景信息。【方法】利用PacBio RS II测序平台对柑橘黄单胞菌杧果致病变种XC01菌株进行全基因组测序,并对测序数据进行序列拼接、基因预测与功能注释、COG/GO聚类分析等。【结果】柑橘黄单胞菌杧果致病变种XC01菌株整个基因组大小为3 865 165 bp,GC含量为68.9%,编码3 442个蛋白基因,共有3 297个基因具有明显的生物学功能,其中150个基因参与碳水化合物代谢,582个基因参与到寄主与病原互作机制中。序列提交至GenBank数据库,登录号为CP016836。【结论】本研究首次报道了杧果细菌性角斑病病原菌的全基因组序列,分析了基因组的基本特征,初步解释了该菌株致病的关键基因,为深入开展该病菌侵染植物的作用机制提供重要的理论基础。

关键词(KeyWords)： 杧果细菌性角斑病;全基因组测序;致病机制

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划(2017YFD0202107);; 广东省现代农业产业技术体系创新团队-优稀水果产业技术体系首席专家(2016LM144);; 海南省自然科学基金(20163109)

作者(Author): 柳凤;欧雄常;詹儒林;

Email:

DOI:

参考文献(References)：

• [1] NORDBERG H,CANTOR M,DUSHEYKO S,HUA S,POLIAKOV A,SHABALOV L,SMIRNOVA T,GRIGORIEV LV,DUBAHAK I. The genome portal of the department of energy joint denome institute:2014 updates[J]. Nucleic Acids Research,2004,42(1):26-31.
• [2] SILBY M W,CERDENO-TARRAGA A M,VERNIKOS G S,GIDDEN S R,JACKSON R W,PRESTIN G M,ZHANG X X.Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens[J]. Genome Biology,2009,10(5):51.
• [3] DEAN R A,TALBOT N J,EBBOLE D J,FARMAN M L,MITCHELL T K,ORBACH M J,THON M,KULKARNI R,XU J R,PAN H,READ N D,LEE Y H,CARBONE L,BROWN D,OH Y Y. The genome sequence of the rice blast fungus Magnaporthe grisea[J]. Nature,2005,434(7036):980-986.
• [4] LEE D G,URBACH J M,WU G,LIBERATI N T,FEINBAUM R L,MIYAYA S,DIGGINS L T,HE J,SAUCIER M,DEZIEL E,FRIEDMAN L,LI L,GRILLS G,MONTGOMERY K. Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial[J]. Genome Biology,2006,7(10):90.
• [5] MARCET H M,BALLESTER A R,FUENTE B,HARRIES E,MARCOS J F,CONZALEZ-CANDELAS L,GABALDON T,ANA-ROSA B,BEATRIZ F. Genome sequence of the necrotrophic fungus Penicillium digitatum,the main postharvest pathogen of citrus[J]. BMC Genomics 2012,13(1):646.
• [6] SALZBERG S L,SOMMER D D,SCHATZ M C,PHILLIPPY A M,RABINOEICZ P D,TSUGE S,FURUTANI A. Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A[J]. BMC Genomics,2008,9(1):204.
• [7]陈相永.大丽轮枝菌不同毒力菌株全基因组测序及重测序分析[D].北京:中国农业科学院,2012.CHEN Xiangyong. The analysis of whole genome sequencing and re-sequencing of diversely virulent isolates of Verticillium dahlia[D]. Beijing:Chinese Academy of Agricultural Sciences,2012.
• [8] HAAS B J,KAMOUN S,ZODY M C,JIANG R H Y,HANDSAKER R E,CANO L M,GRABHER M,KODIRA C D,RAFFAELE S,TORYO-ALALIBO T,BOZKURT T O. Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans[J]. Nature,2009,461(7262):393-398.
• [9]张大智,詹儒林,柳凤,李国平,赵艳龙,常金梅.杧果细菌性角斑病菌细胞壁降解酶致病作用[J].果树学报,2016,33(5):585-593.ZHANG Dazhi,ZHAN Rulin,LIU Feng,LI Guoping,ZHAO Yanlong,CHANG Jinmei. Pathogenic effect of of cell wall degrading enzymes produced by pathogen causing mango bacterial leaf spot[J]. Journal of Fruit Science,2016,33(5):585-593.
• [10]张大智,詹儒林,柳凤,赵艳龙,何衍彪,常金梅.杧果细菌性角斑病菌粗毒素的生物学活性、理化性质及致病作用[J].果树学报,2017,34(6):723-734.ZHANG Dazhi,ZHAN Rulin,LIU Feng,ZHAO Yanlong,HE Yanbiao,CHANG Jinmei. Biological activity,physical and chemical properties and pathogenic effect of crude toxic produced by pathogen causing mango bacterial leaf spot[J]. Journal of Fruit Science,2017,34(6):723-734.
• [11] EID J,FEHR A,GRAY J,LUING K,LYLE J,OTTO G,PELUSO P,RANK D,BAYBAYAN P,BETTMAN B,BIBILLO A,BJORNSON K,CHAUDHURI B,CHRISTIANS F,CICERO R. Real-time DNA sequencing from single polymerase molecules[J]. Science,2009,323(5910):133-138.
• [12] FAINO L,SEIDL M F,DATEMA E,BERG G C M,JANSSEN A,WITTENBERG A H J,THOMMA B P H. Single-molecule real-time sequencing combined with optical mapping yields completely finished fungal genome[J]. MBio,2015,6(4):e00936.
• [13] ZERBINO D R,BIRNER E. Velvet:algorithms for de novo short read assembly using de Bruijn graphs[J]. Genome Research,2008,18(5):821-829.
• [14] HYATT D,CHEN G L,LOCASCIO P F,LAND M L,LARIMER F W,HAUSER L J. Prodigal:prokaryotic gene recognition and translation initiation site identification[J]. BMC Bioinformatics,2010,11(1):119.
• [15] OCHIAI H,INOUE Y,TAKEYA M,SASAKI A,KAKU H. Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity[J]. JARQ,2005,39(4):275-287.
• [16] CAILLAUD M C,ASAI S,RALLAPALLI G,PIQUEREZ S,FABRO G,JONES J D G. A downy mildew effector attenuates salicylic acid-triggered immunity in Arabidopsis by interacting with the host mediator complex[J]. PLoS Biology,2013,11(12):e1001732.
• [17] BIRCH P R,BOEVINK P C,GILROY E M,HEIN I,PRITCHARD L,WHISSON S C. Oomycete RXLR effectors:delivery,functional redundancy and durable disease resistance[J]. Current Opinion in Plant Biology,2008,11(4):373-379.
• [18] ZULUAGA A P,SOLE M,LU H B,GONGORA-GASTILLO E,VAILLANCOURT B,COLL N,BUELL C R,VALLS M.Transcriptome responses to Ralstonia solanacearum infection in the roots of the wild potato Solanum commersonii[J]. BMC Genomics,2015,16(1):246.