杧果细菌性角斑病病原菌XC01菌株的全基因组测序及序列分析Complete genome sequencing of Xanthomonas citri pv. mangiferaeindicae XC01 in mango
柳凤;欧雄常;詹儒林;
摘要(Abstract)：
【目的】由柑橘黄单胞菌杧果致病变种引起的杧果细菌性角斑病是杧果生产上的重要病害,解析杧果细菌性角斑病病原菌(柑橘黄单胞菌杧果致病变种)XC01菌株的基因组序列信息,为深入研究病菌的致病机制提供序列背景信息。【方法】利用PacBio RS II测序平台对柑橘黄单胞菌杧果致病变种XC01菌株进行全基因组测序,并对测序数据进行序列拼接、基因预测与功能注释、COG/GO聚类分析等。【结果】柑橘黄单胞菌杧果致病变种XC01菌株整个基因组大小为3 865 165 bp,GC含量为68.9%,编码3 442个蛋白基因,共有3 297个基因具有明显的生物学功能,其中150个基因参与碳水化合物代谢,582个基因参与到寄主与病原互作机制中。序列提交至GenBank数据库,登录号为CP016836。【结论】本研究首次报道了杧果细菌性角斑病病原菌的全基因组序列,分析了基因组的基本特征,初步解释了该菌株致病的关键基因,为深入开展该病菌侵染植物的作用机制提供重要的理论基础。
关键词(KeyWords)： 杧果细菌性角斑病;全基因组测序;致病机制
基金项目(Foundation): 国家重点研发计划(2017YFD0202107);; 广东省现代农业产业技术体系创新团队-优稀水果产业技术体系首席专家(2016LM144);; 海南省自然科学基金(20163109)
作者(Author): 柳凤;欧雄常;詹儒林;
Email:
DOI: 10.13925/j.cnki.gsxb.20180117
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