杨宏霞;刘冰雁;刘振坤;鹿艳新;王雪;曲柏宏;申永杰;

• 1:延边大学农学院
• 2:沈阳农业大学园艺学院
• 3:延边朝鲜族自治州农业科学院
• 4:延边华龙集团果树农场

摘要(Abstract)：

【目的】克隆‘苹果梨’(Pyrus bretschneideri Rehd.‘Pingguoli’)Py CHI和Py F3H的全长c DNA序列,检测其在果实着色过程中的表达特性及其与花青苷积累的关系。【方法】以‘苹果梨’套袋后去袋处理的果实和未套袋的果实(CK)为试材,采用同源克隆技术克隆Py CHI和Py F3H的全长c DNA序列并进行生物信息预测分析;利用实时荧光定量PCR技术检测Py CHI和Py F3H在去袋处理与未套袋处理的不同着色时期的果实中的表达特性。【结果】Py CHI和Py F3H的全长c DNA分别为702 bp和1095 bp,分别编码233和364个氨基酸。同源性分析显示,其与沙梨、西洋梨等蔷薇科梨属植物相应基因的同源性均超过95%,相应氨基酸序列相似性均达到85%。实时荧光定量PCR分析显示,套袋果实去袋见光后Py CHI和Py F3H表达量均迅速上升,随后Py CHI表达量虽然略有下降,但在整个果实着色过程中Py CHI和Py F3H的表达量一直维持在较高水平,均显著高于对照,且与果实花青苷含量的变化规律基本一致。【结论】获得‘苹果梨’PyCHI和Py F3H的全长c DNA序列,其表达水平与果实花青苷积累关系密切,表明其在调控‘苹果梨’果实着色过程中起重要作用。

关键词(KeyWords)： ‘苹果梨’;花青苷;基因克隆;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31160389)

作者(Author): 杨宏霞;刘冰雁;刘振坤;鹿艳新;王雪;曲柏宏;申永杰;

Email:

DOI: 10.13925/j.cnki.gsxb.20140419

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