范昆;曲健禄;李林光;王涛;秦旭;李晓军;

• 1:山东省果树研究所
• 2:山东省农业干部管理学院

摘要(Abstract)：

【目的】为明确苹果主产区轮纹病菌对戊唑醇的敏感现状,并评价苹果轮纹病菌对戊唑醇的抗性风险,【方法】采用菌丝生长速率法,测定了51株采自山东、河北、陕西等地的苹果轮纹病菌对戊唑醇的敏感性,并通过紫外线诱变野生敏感菌株获得抗药性突变体,进一步利用菌丝生长速率法测定抗性菌株对其他一些杀菌剂的交互抗性并比较其与敏感菌株之间的部分生物学特性。【结果】51株苹果轮纹病菌菌株EC50值分布于0.0111~0.7264 mg.L-1,平均EC50值(0.2541±0.0151)mg.L-1,其对戊唑醇的敏感性呈单峰分布,可作为将来进行田间苹果轮纹病菌对戊唑醇抗药性检测的敏感性基线。通过紫外线诱变野生敏感菌株获得9株抗性突变体,抗性倍数在11.00～67.88。抗性菌株UV-TS1-10对丙环唑等7种药剂表现出了不同程度的交互抗药性。9株抗戊唑醇突变体继代培养至第9代,抗性倍数逐渐下降,表明所获抗性不能稳定遗传。抗药性突变体的菌丝生长速率与亲本菌株无明显差异,而菌丝干重却与亲本菌株相比显著性降低。【结论】苹果轮纹病菌对戊唑醇具有中度至高度抗药性风险,生产中需引起高度重视。

关键词(KeyWords)： 苹果轮纹病菌;戊唑醇;敏感基线;抗药突变体

Abstract:

Keywords:

基金项目(Foundation): 现代苹果产业技术体系建设专项(CARS-28);; 山东省科技发展计划(2012GNC11319)

作者(Author): 范昆;曲健禄;李林光;王涛;秦旭;李晓军;

Email:

参考文献(References)：

• [1]SU Ping,ZHOU Zeng-qiang,ZHU Jian-lan,HOU Hui,WANG Li.Sensitivity of Botryosphaeria berengeriana f.sp.piricola to tebu-conazole[J].Chinese Agricultural Science Bulletin,2010,26(13):312-318.苏平,周增强,朱建兰,侯珲,王丽.河南省苹果轮纹病菌对戊唑醇的敏感性检测[J].中国农学通报,2010,26(13):312-318.
• [2]LI Guang-xu,SHEN Yong-bo,GAO Yan-min,CHEN Jie,JIEYOUL UHM.Study on the infection mechanism of apple ring rot dis-ease[J].Journal of Fruit Science,2007,24(1):16-20.李广旭,沈永波,高艳敏,陈杰,Jie Youl Uhm.苹果轮纹病菌侵染机制的研究[J].果树学报,2007,24(1):16-20.
• [3]YUAN Zhong-lin,LUO Lan,MENG Zhao-li.Control effect of artifi-cial synthesized fungicide Yintai on 2 kinds of apple diseases[J].Ac-ta Phytophylacica Sinica,2006,33(2):223-224.袁忠林,罗兰,孟昭礼.仿生农用杀菌剂银泰对苹果两种主要病害的防治效果[J].植物保护学报,2006,33(2):223-224.
• [4]WANG Ying-zi,ZHANG Wei,LIU Bao-you,LUAN Bing-hui,WANG Pei-song.Research on resistance and geographical distribu-tion of Botryosphaeria dothidea from apple to Tebuconazole in Shan-dong province[J].Journal of Fruit Science,2010,27(6):961-964.王英姿,张伟,刘保友,栾炳辉,王培松.山东省苹果轮纹病菌对戊唑醇的抗药性及其地理分布[J].果树学报,2010,27(6):961-964.
• [5]LI Fu-gen,WU Xin-ping,LIU Nai-chi.The functional characteris-tics and application situation of tebuconazole[J].Pesticide Scienceand Administration,2001,22(3):40-41.李富根,吴新平,刘乃炽.戊唑醇的作用特点及其应用概况[J].农药科学与管理,2001,22(3):40-41.
• [6]LEROUX P,CHAPELAND F,DESBROSSES D,GREDT M.Pat-terns of crossresistance to fungicides in Botryotinia fuckeliana(Botrytis cinerea)isolates from French vineyards[J].Crop Protec-tion,1999,18(10):687-697.
• [7]ROBBERTSE B,VAN DER RIJST M,VAN AARDE I M R,LENNOX C,CROUS P W.DMI sensitivity and cross-resistancepatterns of Rhynchosporium secalis isolates from South Africa[J].Crop Protection,2001,20(2):97-102.
• [8]MA Z H,MORGAN D P,FELTS D,MICHAILIDES T J.Sensitivityof Botryosphaeria dothidea from California pistachio to tebuconazole[J].Crop Protection,2002,21(9):829-835.
• [9]HUANG Guo-yang.Pesticide testing technology and evaluationmethod[M].Beijing:China Agriculture Press,2000.黄国洋.农药试验技术与评价方法[M].北京:中国农业出版社,2000.
• [10]CHAO L J,SHAN X M,CHE S C,ZENG D P.Study on casual a-gent,epidemic law and integrated control techniques of turfgrassbrown patch disease[J].Grassland of China,2000(4):42-47.
• [11]MU Li-yi.Reseach method of Plant Protection[M].Beijing:China A-griculture Press,1994.慕立义.植物化学保护研究方法[M].北京:中国农业出版社,1994.
• [12]CHEN Nian-chun.Bioassay technology of pesticide[M].Beijing:Bei-jing Agricultural University Press,1990.陈年春.农药生物测定技术[M].北京:北京农业大学出版社,1990.
• [13]BRUIN G C A,EDGINGTON L V.Induction of fungal resistance tometalaxyl by ultraviolet irradiation[J].Phytopathology,1982,72(5):1209-1212.
• [14]YE Tao,MA Zhi-qiang,WANG Wen-qiao,HAN Xiu-ying,ZHANGXiao-feng.Resistance induction of Fusarium graminearum to tebu-conazole and bionomics of resistant-mutant[J].Chinese Journal ofPesticide Science,2011,13(3):261-266.叶滔,马志强,王文桥,韩秀英,张小风.禾谷镰孢菌对戊唑醇抗药性的诱导及抗药性菌株特性研究[J].农药学学报,2011,13(3):261-266.
• [15]LI Heng-kui,CHEN Chang-jun,WANG Jian-xin,ZHOU Ming-guo.Study on baseline-sensitivity of Fusarium graminearum to JS399-19and assessment of the risk of resistance in vitro[J].Acta Phytopatho-logica Sinica,2006,36(3):273-278.李恒奎,陈长军,王建新,周明国.禾谷镰孢菌对氰烯菌酯的敏感性基线及室内抗药性风险初步评估[J].植物病理学报,2006,36(3):273-278.
• [16]YANG Lian-lai,LIU Wei-wei,LIU Yue-qun,MU Wei,LIU Feng.Baseline sensitivity and acquisition of resistance of Sphaerothecafuliginea to hexaconazole in lab[J].Acta Phytophylacica Sinica,2007,34(4):431-435.杨连来,刘玮玮,刘跃群,慕卫,刘峰.黄瓜白粉病菌对己唑醇的敏感基线及实验室抗性获得[J].植物保护学报,2007,34(4):431-435.
• [17]FAO.Recommended methods for the detection and measurementof resistance of resistance of agricultural pests to pesticides[J].FAOPlant Prot Bull,1982,30(2):30-36.
• [18]LIU Ying-hua,WANG Kai-yun,JIANG Xing-yin,YI Mei-qin,WANG Huai-xun.Resistance of Rhizoctonia cerealis to tebucona-zole and the biological characters of tebuconazole-resistant strains[J].Journal of Plant Protection,2003,30(4):423-428.刘英华,王开运,姜兴印,仪美琴,王怀训.禾谷丝核菌对戊哇醇的抗性及抗药性菌系生物学特性[J].植物保护学报,2003,30(4):423-428.
• [19]WANG Han-cheng,ZHOU Ming-guo,ZHANG Yan-jun,CHENChang-jun,WANG Jian-xin.Fungicidal activity of tebuconazole a-gainst Rhizoctonia solani and its application to rice[J].Chinese Jour-nal of Pesticide Science,2007,9(4):357-362.汪汉成,周明国,张艳军,陈长军,王建新.戊唑醇对立枯丝核菌的抑制作用及在水稻上的应用[J].农药学学报,2007,9(4):357-362.
• [20]HU Yan,WANG Huai-xun,XIA Xiao-ming,WANG Kai-yun,WANG Hong-yan.Susceptibility of Rhizoctonia cerealis to sixfungicides in four wheat areas[J].Acta Phytophylacica Sinica,2006,33(4):55-60.胡燕,王怀训,夏晓明,王开运,王红艳.四地区小麦纹枯病菌对6种杀菌剂的抗性比较[J].植物保护学报,2006,33(4):55-60.
• [21]PENG Bin,LIU Li-feng,WU Hui-jie,TIAN Li-li,ZHOU Zeng-qiang.The intraspecific genetic diversity of pathogenic fungi of appleRing Rot[J].Scientia Agricultura Sinica,2011,44(6):1125-1135.彭斌,刘丽锋,吴会杰,田莉莉,周增强.苹果轮纹病菌种内遗传多样性研究[J].中国农业科学,2011,44(6):1125-1135.
• [22]ZHAO Na,ZHANG Wei-wei,GAO Xiao-ning,LIN Yue-li,KANG Zhen-sheng,HUANG Li-li.Phylogenetic relationships ofBotryosphaeria dothidea and its allies in China[J].Journal of north-west A&F University:Natural Science Edition,2011,39(10):123-133.赵娜,张伟伟,高小宁,林月莉,康振生,黄丽丽.中国部分省份苹果轮纹病菌及相关类群的系统学关系[J].西北农林科技大学学报:自然科学版,2011,39(10):123-133.
• [23]FUCHS A,DRANDAREVSKI C A.The likelihood of decelopment ofresistance to systemic fungicides which inhibit ergosterol biosynthe-sis[J].Neth J Plant Pahtol,1976,82:85-87.
• [24]KLLER W,SCHEINPFLUG H.Fungal resistance to sterol biosyn-thesis inhibitors:a new challenge[J].Plant Disease,1987,71:1066-1074.
• [25]LIN Cai-hua,WANG Kai-yun,GU Chun-bo,ZUO Yi-ming,NIUFang.Sensitivities of Fusarium oxysporum f.sp.fragariae to four tri-azole fungicides in major strawberry-growing areas of ShandongProvince[J].Acta Phytophylacica Sinica,2009,36(1):55-60.林才华,王开运,顾春波,左一鸣,牛芳.山东省草莓枯萎病菌对四种三唑类杀菌剂的敏感性检测[J].植物保护学报,2009,36(1):55-60.
• [26]AKALLAL R,DEBIEU D,LANEN C,DABOUSSI M-J,FRITZ R,MALOSSE C,BACH J,LEROUX P.Inheritance and mechanisms ofresistance to tebuconazole,a sterol C14-demethylation inhibitor,inNectria haematococca[J].Pesticide Biochemistry and Physiology,1998,60(3):147-166.
• [27]LI Xiao-jun,FAN Kun,QU Jian-lu,ZHANG Yong,WANG Tao.De-termination of the sensitivity of Botryosphaeria berengriana to Car-bendazim fungicide[J].Journal of Fruit Science,2009,26(4):516-519.李晓军,范昆,曲健禄,张勇,王涛.苹果轮纹病菌对多菌灵的敏感性测定[J].果树学报,2009,26(4):516-519.
• [28]LI Xiao-jun,FAN Kun,QU Jian-lu,ZHANG Yong,WANG Tao.De-termination on Sensitivities of Botryosphaeria berengriana to iprodi-one[J].Agrochemicals,2009,48(10):774-776.李晓军,范昆,曲健禄,张勇,王涛.苹果轮纹病菌对异菌脲的敏感性测定[J].农药,2009,48(10):774-776.