钙处理对葡萄果实花青素含量及品质的影响Effects of calcium treatments on grape fruit quality and anthocyanin content
余俊;赵玉华;白描;杨国顺;
摘要(Abstract):
【目的】通过研究不同钙处理对葡萄花青素合成及品质的影响,确定适宜的喷钙时期、浓度、部位,为葡萄的优质高产提供理论依据。【方法】以6 a(年)生‘美人指’为试验材料,研究了不同钙处理水平(0、1、3、5、8 g·L~(-1))、施肥方式(叶果喷施和浸果)及时期(第1次膨大期和第2次膨大期)对葡萄果实品质和花青素含量的影响。【结果】钙处理可明显提高果实的单粒质量、可溶性固形物及花青素含量,并降低果实硬度和酸度,果实品质和花青素含量随着钙质量浓度的增加而提高,在5 g·L~(-1)时效果最好,同时还发现钙处理效果在葡萄第2次膨大期优于第1次膨大期,浸果处理优于叶果喷施处理。第2次膨大期5 g·L~(-1)钙溶液浸果处理的花青素含量为820.23 nmol·g~(-1)(约为对照的5.72倍)、可溶性固形物含量(ω)为16.23%(约为对照的1.28倍)、固酸比为63.40(约为对照的1.67倍),而总酸含量(ω)显著低于其他处理,为0.26%(约为对照的0.76倍)。【结论】在葡萄第2次膨大期采用5 g·L~(-1)钙溶液浸果处理,对于提高葡萄品质和果实花青素含量最为有利。
关键词(KeyWords): 葡萄;钙;果实品质;花青素
基金项目(Foundation): 国家葡萄产业技术体系(CARS-29-ZP-9);; 湖南省重点研发项目(2013FJJ6102)
作者(Author): 余俊;赵玉华;白描;杨国顺;
Email:
DOI: 10.13925/j.cnki.gsxb.20170449
参考文献(References):
- [1]JAAKOLA L.New insights into the regulation of anthocyanin biosynthesis in fruits[J].Trends in Plant Science,2013,18(9):477.
- [2]PETRONI K,TONELLI C.Recent advances on the regulation of anthocyanin synthesis in reproductive organs[J].Plant Science,2011,181(3):219-229.
- [3]KUROSAKI F,TSURUSAWA Y,NISHI A.The elicitation of phytoalexins by Ca2+,and cyclic AMP in carrot cells[J].Phytochemistry,1987,26(7):1919-1923.
- [4]MURATA Y,PEI Z M,MORI I C,SCHROEDER J.Abscisic acid activation of plasma membrane Ca2+channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants[J].Plant Cell,2001,13(11):2513.
- [5]SUDHA G.The role of calcium channels in anthocyanin production in callus cultures of Daucus carota[J].Plant Growth Regulation,2003,40(2):163-169.
- [6]马廷蕊.NO和H2O2对马铃薯花青素合成调控及信号转导[D].兰州:甘肃农业大学,2013.MA Tingrui.Analysis of exogenous NO and H2O2regulation to potato anthocyanin synthesis and its signal transduction[D].Lanzhou:Gansu Agricultural University,2013.
- [7]夏春华,蔡世英.GA3和Ca2+对安祖花佛焰苞花青苷含量及花梗PAL活性的影响[J].园艺学报,2004,31(3):343-344.XIA Chunhua,CAI Shiying.Effects of GA3,Ca2+on anthocyanin and phenylalanine amonia lyase in stem of Anthurium andraeanum[J].Acta Horticulturae Sinica,2004,31(3):343-344.
- [8]车玉红.钙肥对红富士苹果果实品质及生理生化特性影响的研究[D].杨凌:西北农林科技大学,2005.CHE Yuhong.Study on the effect of calcium fertilization on fruit quality and physiological and biochemical characteristics of Red Fuji apple[D].Yangling:Northwest Agriculture and Forestry University,2005.
- [9]周开兵,苏举,徐远锋.磷、钾和钙元素对三月红荔枝果皮着色的影响[J].中国土壤与肥料,2007(6):54-57.ZHOU Kaibing,SU Ju,XU Yuanfeng.Effects of P,K and Ca on the pigmentation of sanyuehong litchi(Litchi chinensis Sonn.cv.Sanyuehong)fruits[J].Soil and Fertilizer Sciences in China,2007(6):54-57.
- [10]郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2007:80-186.HAO Jianjun,KANG Zongli,YU Yang.Plant physiology experiment technology[M].Beijing:Chemical Industry Press,2007:80-186.
- [11]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007:110-125.CAO Jiankang,JIANG Weibo,ZHAO Yumei.Physiological and biochemical experimental guidance for fruits and vegetables[M].Beijing:China Light Industry Press,2007:110-125.
- [12]BUSH D S.Calcium regulation in plant cells and its role in signaling[J].Annual Review of Plant Physiology&Plant Molecular Biology,1995,46(1):95-122.
- [13]BOWLER C,FLUHR R.The role of calcium and activated oxygens as signals for controlling cross-tolerance[J].Trends in Plant Science,2000,5(6):241.
- [14]CICCARESE A,STELLACCI A M,GENTILESCO G,RUBINO P.Effectiveness of pre-and post-veraison calcium applications to control decay and maintain table grape fruit quality during storage[J].Postharvest Biology&Technology,2013,75(1):135-141.
- [15]王强,罗巨海,李磊,王平,张静.果蔬钙肥在葡萄生产上的应用试验[J].西北园艺,2010(4):41-42.WANG Qiang,LUO Juhai,LI Lei,WANG Ping,ZHANG Jing.Application of calcium fertilizer in grape production[J].Northwest Horticulturae,2010(4):41-42.
- [16]刘鑫铭,陈婷,雷龑,黄新忠,蔡盛华.施钙处理对夏黑葡萄成熟过程果实品质及贮藏性的影响[J].福建农业学报,2013,28(12):1252-1256.LIU Xinming,CHEN Ting,LEI Yan,HUANG Xinzhong,CAI Shenghua.Effects of calcium treatments on quality of Xiahei grape during ripening and storage property[J].Fujian Journal of Agricultural Science,2013,28(12):1252-1256.
- [17]LO G D,WOLF T K,ZOECKLEIN B W.Effects of prohexadione-calcium on grape yield components and fruit and wine composition[J].American Journal of Enology&Viticulture,2004,55(1):73-83.
- [18]XU W P,PENG H,YANG T B,WHITAKER B,HUANG L H,SUN J H,CHEN P.Effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation[J].Plant Physiology&Biochemistry,2014,82(3):289-298.
- [19]WANG C Q,ZHANG Y F,LIU T.Activity changes of Ca M and Ca2+-ATPase during low temperature-induced anthocyanin accumulation in Alternanthera bettzickiana[J].Physiologia Plantarum,2005,124(2):260-266.
- [20]VITRAC X,LARRONDE F,KRISA S,DECENDIT A,DEFFIEUX G,MERILLON J M.Sugar sensing and Ca2+-calmodulin requirement in Vitis vinifera cells producing anthocyanins[J].Phytochemistry,2000,53(6):659.
- [21]SHIN D H,CHOI M G,LEE H K,CHO M,CHOI S B,CHOI G,PARK Y I.Calcium dependent sucrose uptake links sugar signaling to anthocyanin biosynthesis in Arabidopsis[J].Biochemical&Biophysical Research Communications,2013,430(2):634-639.