孙婷梅;王璐平;张永利;秦家慧;于梦楠;李爱;彭立新;

• 1:天津农学院园艺园林学院果树学重点实验室
• 2:天津市汉邦植物保护剂有限责任公司

摘要(Abstract)：

【目的】提高珠美海棠的耐盐性,建立更高效的珠美海棠遗传转化体系。【方法】在转化液中利用超声波直接转化无菌条件下横切主脉的珠美海棠试管苗幼叶,通过愈伤组织培养诱导植株再生、抗性筛选,GUS染色鉴定,RT-PCR分析转基因植株的耐盐性。【结果】较利于珠美海棠愈伤组织诱导分化及抗性筛选的培养基为MS+6-BA 2.0 mg·L~(-1)+NAA 0.1 mg·L~(-1)+Kana 50 mg·L~(-1)。超声波处理的最优工作条件为:处理6 s,间歇10 s,工作重复20次,功率80 W,转化效率为31.3%。GUS染色与RT-PCR鉴定结果一致率为100%,RT-PCR结果分析显示,Mz2NHX1基因在转化苗中的表达量约为对照的3倍。荧光定量PCR结果显示转化苗Mz2NHX1基因的表达量为对照组的6.21倍,盐处理试验结果显示转化苗耐盐性得到了显著地提高。【结论】建立了超声波介导的珠美海棠高效遗传转化体系,获得珠美海棠耐盐新材料,为该耐盐基因的研究和盐碱地的改良奠定了基础。

关键词(KeyWords)： 珠美海棠;遗传转化体系;超声波;植株再生;耐盐基因;过表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30671440,31300564)

作者(Author): 孙婷梅;王璐平;张永利;秦家慧;于梦楠;李爱;彭立新;

Email:

DOI: 10.13925/j.cnki.gsxb.20160063

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