利用拮抗酵母防治水果采后病害的研究进展Advances in the application of antagonistic yeasts to manage postharvest diseases in fruit
喻最新;贺明阳;王日葵;韩冷;周炼;
摘要(Abstract):
随着人们安全意识的提高,化学杀菌剂由于安全性差、且具致癌性等问题逐渐被淘汰,新型绿色、环保的生物防治逐渐成为当下研究的热点。拮抗酵母以其来源广泛、对营养条件要求低、生长迅速、安全性高等特点,在水果采后病害防治方面应用广泛。笔者在对拮抗酵母防治水果采后病害的来源途径、可能的拮抗机制以及增效途径进行综述的基础上,针对限制拮抗酵母商业化应用的原因进行了分析,即目前对拮抗酵母拮抗机制的研究还有待深入,同时工业上大规模生产的拮抗酵母制剂很难实现在实验室规模下发酵所具有的性质。通过组学等新的技术进一步揭示拮抗酵母防治采后病害的作用机制,进一步探讨拮抗酵母的分离干燥方式、佐剂以及剂型的选择,逐步完善适合拮抗酵母特性的商业化生产、销售方法和技术。
关键词(KeyWords): 水果;拮抗酵母;生物防治;组学技术;商业化应用
基金项目(Foundation): 重庆市科技专项(cstc2016shms-ztzx80005);; 国家科技支撑计划(2015BAD16B07);; 中央高校基本科研业务费(XDJK2017C016);; 重庆市重点实验室基金(CKLC201302)
作者(Authors): 喻最新;贺明阳;王日葵;韩冷;周炼;
DOI: 10.13925/j.cnki.gsxb.20170385
参考文献(References):
- [1]胡浩.西藏罗伦隐球酵母对水果采后病害的防治效果及相关机制研究[D].杭州:浙江大学,2016.HU Hao.Biocontrol of fruit postharvest decay by Tibetan Cryptococcus laurentii and its action mechanism[D].Hangzhou:Zhe jiang University,2016.
- [2]ZHANG H,APALIYA M T,MAHUNU G K,CHEN L,LI W.Control of ochratoxin A-producing fungi in grape berry by microbial antagonists:A review[J].Trends in Food Science&Technology,2016,51:88-97.
- [3]盖智星,王日葵,贺明阳,周炼,韩冷,黄小兰.肉桂酸钾对柑橘采后主要病原真菌的抑制作用[J].食品与发酵工业,2016,42(3):109-113.GE Zhixing,WANG Rikui,HE Mingyang,ZHOU Lian,HANLeng,HUANG Xiaolan.Antifungal acitivity of potassium cinnamate against the main postharvest fungal pathogens of citrus fruits[J].Food and Fermentation Industries,2016,42(3):109-113.
- [4]YANG Q,WANG H,ZHANG H,ZHANG X,APALIYA M T,ZHENG X,MAHUNU G K.Effect of Yarrowia lipolytica on postharvest decay of grapes caused by Talaromyces rugulosus and the protein expression profile of T.rugulosus[J].Postharvest Biology and Technology,2017,126:15-22.
- [5]DE PAIVA E,SERRADILLA M J,RUIZ-MOYANO S,CóRDOBA M G,VILLALOBOS M C,CASQUETE R,HERNáNDEZ A.Combined effect of antagonistic yeast and modified atmosphere to control Penicillium expansum infection in sweet cherries cv.Ambrunés[J].International Journal of Food Microbiology,2017,241:276-282.
- [6]DROBY S,WISNIEWSKI M,TEIXIDóN,SPADARO D,JIJAKLI M H.The science,development,and commercialization of postharvest biocontrol products[J].Postharvest Biology and Technology,2016,122:22-29.
- [7]张红印,蒋益虹,郑晓冬,席玙芳,孙萍.酵母菌对果蔬采后病害防治的研究进展[J].农业工程学报,2003,19(4):23-27.ZHANG Hongyin,JIANG Yihong,ZHENG Xiaodong,XI Yufang,SUN Ping.Research advances on the biological control of postharvest diseases of fruits and vegetables with yeast[J].Transactions of the Chinese Society of Agricultural Engineering,2003,19(4):23-27.
- [8]耿鹏,杨柳,郝卫宁,李辉,胡美英.拮抗酵母菌控制果蔬采后病害的研究进展[J].北方园艺,2010(20):220-225.GENG Peng,YANG Liu,HAO Weining,LI Hui,HU Meiying.Research advances on the biological control of postharvest diseases of fruits and vegetables with antagonistic yeasts[J].Northern Horticulture,2010(20):220-225.
- [9]林晓敏,谭晓荣.拮抗酵母菌防治果蔬采后病害的研究[J].北方园艺,2015(1):182-186.LIN Xiaomin,TAN Xiaorong.Study on antagonistic yeasts control of biological postharvest diseases of fruit and vegetables[J].Northern Horticulture,2015(1):182-186.
- [10]BASTIAANSE H,DE LAPEYRE DE BELLAIRE L,LASSOISL,MISSON C,JIJAKLI M H.Integrated control of crown rot of banana with Candida oleophila strain O,calcium chloride and modified atmosphere packaging[J].Biological Control,2010,53(1):100-107.
- [11]ZENG L,YU C,FU D,LU H,ZHU R,LU L,ZHENG X,YU T.Improvement in the effectiveness of Cryptococcus laurentii to control postharvest blue mold of pear by its culture inβ-glucan amended nutrient broth[J].Postharvest Biology and Technology,2015,104:26-32.
- [12]CAMPOS-MARTíNEZ A,VELáZQUEZ-DEL VALLE M G,FLORES-MOCTEZUMA H E,SUáREZ-RODRíGUEZ R,RAMíREZ-TRUJILLO J A,HERNáNDEZ-LAUZARDO A N.Antagonistic yeasts with potential to control Colletotrichum gloeosporioides(Penz.)Penz.&Sacc.and Colletotrichum acutatum J.H.Simmonds on avocado fruits[J].Crop Protection,2016,89:101-104.
- [13]GUO D,YANG B,REN X,ZHU L.Expression of the Le PR5gene from cherry tomato fruit induced by Cryptococcus laurentii and the analysis of Le PR5 protein antifungal activity[J].Postharvest Biology and Technology,2016,111:337-344.
- [14]LI G,CHI M,CHEN H,SUI Y,LI Y,LIU Y,ZHANG X,SUNZ,LIU G,WANG Q,LIU J.Stress tolerance and biocontrol performance of the yeast antagonist,Candida diversa,change with morphology transition[J].Environmental Science and Pollution Research,2016,23(3):2962-2967.
- [15]ZHIMO V Y,DILIP D,STEN J,RAVAT V K,BHUTIA D D.Antagonistic yeasts for biocontrol of the banana postharvest anthracnose pathogen colletotrichum musae[J].Journal of Phytopathology,2017,165(1):35-43.
- [16]LONG C,WU Z,DENG B.Biological control of Penicillium italicum of Citrus and Botrytis cinerea of grape by strain 34-9 of Kloeckera apiculata[J].European Food Research and Technology,2005,221(1/2):197-201.
- [17]VERO S,GARMENDIA G,GONZALEZ M B,BENTANCURO,WISNIEWSKI M.Evaluation of yeasts obtained from antarctic soil samples as biocontrol agents for the management of postharvest diseases of apple(Malus×domestica)[J].FEMS Yeast Research,2013,13(2):189-199.
- [18]LU L,WANG J,ZHU R,LU H,ZHENG X,YU T.Transcript profiling analysis of Rhodosporidium paludigenum-mediated signalling pathways and defense responses in mandarin orange[J].Food Chemistry,2015,172(172):603-612.
- [19]MAHUNU G K,ZHANG H,YANG Q,ZHANG X,LI D,ZHOU Y.Improving the biocontrol efficacy of Pichia caribbica with phytic acid against postharvest blue mold and natural decay in apples[J].Biological Control,2016,92(1):172-180.
- [20]ARRARTE E,GARMENDIA G,ROSSINI C,WISNIEWSKIM,VERO S.Volatile organic compounds produced by antarctic strains of Candida sake play a role in the control of postharvest pathogens of apples[J].Biological Control,2017,109(1):14-20.
- [21]KALOGIANNIS S,TJAMOS S E,STERGIOU A,ANTONIOUP P,ZIOGAS B N.Selection and evaluation of phyllosphere yeasts as biocontrol agents against grey mould of tomato[J].European Journal of Plant Pathology,2006,116(1):69-76.
- [22]HU H,XU Y,LU H,XIAO R,ZHENG X,YU T.Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits[J].Journal of Zhejiang Universityence B,2015,16(4):275-285.
- [23]WEI Y,XU M,WU H,TU S,PAN L,TU K.Defense response of cherry tomato at different maturity stages to combined treatment of hot air and Cryptococcus laurentii[J].Postharvest Biology and Technology,2016,117:177-186.
- [24]刘海波,田世平.水果采后生物防治拮抗机制的研究进展[J].植物学报,2001,18(6):657-664.LIU Haibo,TIAN Shiping.Recent advances on the mechanism of postharvest biocontrol[J].Chinese Bulletin of Botany,2001,18(6):657-664.
- [25]ZHANG D,SPADARO D,GARIBALDI A,GULLINO M L.Potential biocontrol activity of a strain of Pichia guilliermondii against grey mold of apples and its possible modes of action[J].Biological Control,2011,57(3):193-201.
- [26]KWASIBORSKI A,BAJJI M,RENAUT J,DELAPLACE P,JIJAKLI M H.Identification of metabolic pathways expressed by Pichia anomala Kh6 in the presence of the pathogen Botrytis cinerea on apple:new possible targets for biocontrol improvement[J].PLo S One,2014,9(3):e91434.
- [27]SPADARO D,DROBY S.Development of biocontrol products for postharvest diseases of fruit:The importance of elucidating the mechanisms of action of yeast antagonists[J].Trends in Food Science&Technology,2016,47(49):39-49.
- [28]CHI M,LI G,LIU Y,LIU G,LI M,ZHANG X,SUN Z,SUI Y,LIU J.Increase in antioxidant enzyme activity,stress tolerance and biocontrol efficacy of Pichia kudriavzevii with the transition from a yeast-like to biofilm morphology[J].Biological Control,2015,90(2):113-119.
- [29]杨东升,罗先群,王新广.嗜杀酿酒酵母对海南山兰甜酒发酵品质的影响[J].中国酿造,2013,32(11):59-63.YANG Dongsheng,LUO Xianqun,WANG Xinguang.Effect of killer brewery yeast on the fermentation quality of Hainan shanlan rice wine[J].China Brewing,2013,32(11):59-63.
- [30]BREINIG F,TIPPER D J,SCHMITT M J.Kre1p,the plasma membrane receptor for the yeast K1 viral toxin[J].Cell,2002,108(3):395-405.
- [31]FRIEL D,GOMEZ PESSOA N M,VANDENBOL M,JIJAKLIM H.Separate and combined disruptions of two exo-β-1,3-glucanase genes decrease the efficiency of Pichia anomala(strain K)biocontrol against Botrytis cinerea on apple[J].Molecular Plantmicrobe Interactions,2007,20(4):371-379.
- [32]BANANI H,SPADARO D,ZHANG D,MATIC S,GARIBAL-DI A,GULLINO M L.Biocontrol activity of an alkaline serine protease from Aureobasidium pullulans expressed in Pichia pastoris against four postharvest pathogens on apple[J].International Journal of Food Microbiology,2014,182-183(6194):1-8.
- [33]BANANI H,SPADARO D,ZHANG D,MATIC S,GARIBAL-DI A,GULLINO M L.Postharvest application of a novel chitinase cloned from Metschnikowia fructicola and overexpressed in Pichia pastoris to control brown rot of peaches[J].International Journal of Food Microbiology,2015,199:54-61.
- [34]LIU P,CHENG Y,YANG M,LIU Y,CHEN K.Mechanisms of action for 2-phenylethanol isolated from Kloeckera apiculata,in control of Penicillium,molds of citrus fruits[J].BMC Microbiology,2014,14(1):242.
- [35]HUANG R,CHE H J,ZHANG J,YANG L,JIANG D H,LI GQ.Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberry fruits[J].Biological Control,2012,62(1):53-63.
- [36]LU L,YE C,GUO S,SHENG K,SHAO L,ZHOU T,YU T,ZHENG X.Preharvest application of antagonistic yeast Rhodosporidium paludigenum induced resistance against postharvest diseases in mandarin orange[J].Biological Control,2013,67(2):130-136.
- [37]DROBY S,VINOKUR V,WEISS B,COHEN L,DAUS A,GOLDSCHMIDT E E,PORAT R.Induction of resistance to Penicillium digitatum in grapefruit by the yeast biocontrol agent Candida oleophila[J].Phytopathology,2002,92(4):393-399.
- [38]CHAN Z,QIN G,XU X,LI B,TIAN S.Proteome approach to characterize proteins induced by antagonist yeast and salicylic acid in peach fruit[J].Journal of Proteome Research,2007,6(5):1677-1688.
- [39]薛耀碧,周雅涵,邓丽莉,曾凯芳.采后果实病程相关蛋白研究进展[J].食品工业科技,2015,36(6):391-394.XUE Yaobi,ZHOU Yahan,DENG Lili,ZENG Kaifang.Advances on pathogenesis-related proteins of postharvest fruits[J].Science and Technology of Food Industry,2015,36(6):391-394.
- [40]ZHANG H,CHEN L,SUN Y,ZHAO L,ZHENG X,YANG Q,ZHANG X.Investigating proteome and transcriptome defense response of apples induced by Yarrowia lipolytica[J].Molecular Plant-Microbe Interactions,2017,30(4):301-311.
- [41]MASSART S,MARTINEZ-MEDINA M,JIJAKLI M H.Biological control in the microbiome era:Challenges and opportunities[J].Biological Control,2015,89:98-108.
- [42]MASSART S,JIJAKLI H M.Use of molecular techniques to elucidate the mechanisms of action of fungal biocontrol agents:A review[J].Journal of Microbiological Methods,2007,69(2):229-241.
- [43]MASSART S,PERAZZOLLI M,HOFTE M,PERTOT I,JIJAKLI M H.Impact of the omic technologies for understanding the modes of action of biological control agents against plant pathogens[J].Biocontrol,2015,60(6):725-746.
- [44]WANG Y,WANG P,XIA J,YU T,LOU B.Effect of water activity on stress tolerance and biocontrol activity in antagonistic yeast Rhodosporidium paludigenum[J].International Journal of Food Microbiology,2010,143(3):103-108.
- [45]LIU J,WISNIEWSKI M,DROBY S,TIAN S,HERSHKOVITZV,TWORKOSKI T.Effect of heat shock treatment on stress tolerance and biocontrol efficacy of Metschnikowia fructicola[J].Fems Microbiology Ecology,2011,76(1):145-155.
- [46]LIU J,WISNIEWSKI M,DROBY S,NORELLI J,HERSHKO-VITZ V,TIAN S,FARRELL R.Increase in antioxidant gene transcripts,stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure[J].Fems Microbiology Ecology,2012,80(3):578-590.
- [47]CHENG Z,CHI M,LI G,CHEN H,SUI Y,SUN H,WIS-NIEWSKI M,LIU Y,LIU J.Heat shock improves stress tolerance and biocontrol performance of Rhodotorula mucilaginosa[J].Biological Control,2016,95:49-56.
- [48]ZHAO Y,TU K,SU J,TU S,HOU Y,LIU F,ZOU X.Heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested cherry tomato fruit[J].Journal of Agricultural and Food Chemistry,2009,57(16):7565-7570.
- [49]OU C,LIU Y,WANG W,DONG D.Integration of UV-C with antagonistic yeast treatment for controlling post-harvest disease and maintaining fruit quality of Ananas comosus[J].Biocontrol,2016,61(5):591-603.
- [50]SUI Y,LIU J.Effect of glucose on thermotolerance and biocontrol efficacy of the antagonistic yeast Pichia guilliermondii[J].Biological Control,2014,74(3):59-64.
- [51]LIU J,WISNIEWSKI M,DROBY S,VERO S,TIAN S,HERSHKOVITZ V.Glycine betaine improves oxidative stress tolerance and biocontrol efficacy of the antagonistic yeast Cystofilobasidium infirmominiatum[J].International Journal of Food Microbiology,2011,146(1):76-83.
- [52]LU H,LU L,ZENG L,FU D,XIANG H,YU T,ZHENG X.Effect of chitin on the antagonistic activity of Rhodosporidium paludigenum against Penicillium expansum in apple fruit[J].Postharvest Biology and Technology,2014,92:9-15.
- [53]LI W,ZHANG H,LI P,APALIYA M T,YANG Q,PENG Y,ZHANG X.Biocontrol of postharvest green mold of oranges by Hanseniaspora uvarum Y3 in combination with phosphatidylcholine[J].Biological Control,2016,103:30-38.
- [54]FU D,ZENG L,ZHENG X,YU T.Effect ofβ-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear fruit[J].Bio Control,2015,60(5):669-679.
- [55]CHAN Z,TIAN S.Induction of H2O2-metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit[J].Postharvest Biology and Technology,2006,39(3):314-320.
- [56]YU T,CHEN J,CHEN R,HUANG B,LIU D.Biocontrol of blue and gray mold diseases of pear fruit by integration of antagonistic yeast with salicylic acid[J].International Journal of Food Microbiology,2007,116(3):339-345.
- [57]GUO J,FANG W,LU H,ZHU R,LU L,ZHENG X,YU T.Inhibition of green mold disease in mandarins by preventive applications of methyl jasmonate and antagonistic yeast Cryptococcus laurentii[J].Postharvest Biology and Technology,2014,88:72-78.
- [58]YANG Q,LI Y,LI C,ZHANG H,JIANG Z,ZHANG X,SER-WAH B N A,MAHUNU G K,ZHU S.Antioxidative enzymes and substances involve in the activity of improving the oxidative tolerance of Pichia caribbica by ascorbic acid[J].Biological Control,2017,108:83-88.
- [59]MAHUNU G K,ZHANG H,YANG Q,ZHANG X,LI D,ZHOU Y.Improving the biocontrol efficacy of Pichia caribbica with phytic acid against postharvest blue mold and natural decay in apples[J].Biological Control,2016,92(1):172-180.
- [60]LIU J,SUI Y,WISNIEWSKI M,DROBY S,LIU Y.Review:Utilization of antagonistic yeasts to manage postharvest fungal diseases of fruit[J].International Journal of Food Microbiology,2013,167(2):153-160.
- [61]DROBY S,WISNIEWSKI M,MACARISIN D,WILSON C.Twenty years of postharvest biocontrol research:Is it time for a new paradigm?[J].Postharvest Biology and Technology,2009,52(2):137-145.