低温胁迫下4种苹果砧木叶片多胺的变化Changes of polyamines in the leaves of 4 apple rootstocks during low temperature stress
赵玲玲;宋来庆;刘志;谢兴斌;翟衡;郝玉金;
摘要(Abstract):
以山定子(Malus baccata Borkh)、新疆野苹果[M.sieversii(Ledeb)Roem]、烟台沙果[M.prunifoli(Willd)Borkh]和莱芜难咽(M.micromalus Makino)等4种苹果砧木为试材,测定了低温胁迫下不同砧木叶片的多胺含量。结果表明,胁迫6 h时叶片的MDA含量可反映4个砧木的耐寒性,从高到低依次为山定子>新疆野苹果>莱芜难咽>烟台沙果;低温胁迫能明显诱导多胺总量(PAs)、腐胺(Put)、亚精胺(Spd)和精胺(Spm)的合成,耐寒性强的山定子和新疆野苹果PAs、Put和Spd增加显著,而耐寒性较弱的莱芜难咽和烟台沙果多胺和3种胺的变化不大。低温胁迫6 h时,MDA变化量与叶片PAs、Put、Spd变化量以及Put/PAs比值呈极显著或显著负相关,而与(Spd+Spm)/Put、Spd/PAs、Spm/PAs呈显著正相关,与Spm变化量无相关关系,表明当低温胁迫下叶片PAs、Put、Spd增加量较大时,苹果砧木耐低温胁迫的能力比较强,PAs、Put及Spd在胁迫条件下的增加量可作为苹果砧木耐低温胁迫能力高低的鉴定指标。
关键词(KeyWords): 苹果;砧木;低温胁迫;多胺;MDA
基金项目(Foundation): 高等学校全国优秀博士学位论文作者专项资金(200459);; 新世纪优秀人才支持计划
作者(Author): 赵玲玲;宋来庆;刘志;谢兴斌;翟衡;郝玉金;
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参考文献(References):
- [1]LI Bo,LIU Cheng-lian,YANG Rui-hong,LIU Qing-zhong.Studyon the cold resistance ofcherryrootstocks[J].Journal of Fruit Science,2006,23(2):196-199.李勃,刘成连,杨瑞红,刘庆忠.樱桃砧木抗寒性鉴定[J].果树学报,2006,23(2):196-199.
- [2]LI Rong-fu,JIANG Qin-xian,LIANG Yan-rong,LIANG Li,GUOXi-ping.Identification on cold resistance of apple rootstock in field[J].Inner Mongolia Agricultural Science and Technology,2003,6:7-8.李荣富,蒋亲贤,梁艳荣,梁莉,郭喜平.苹果砧木的抗寒性田间鉴定[J].内蒙古农业科技,2003,6:7-8.
- [3]CHEN Shu-fang,ZHU Yue-lin,LIU You-liang,HU Chun-mei,ZHANGGu-wen.Effects of Na Cl stress on ABAand polyamine con-tents in leaves ofgrafted tomatoseedlings[J].Acta Horticulturae Sini-ca,2006,33(1):58-62.陈淑芳,朱月林,刘友良,胡春梅,张古文.Na Cl胁迫对番茄嫁接苗叶片ABA和多胺含量的影响[J].园艺学报,2006,33(1):58-62.
- [4]IRE#NE H,ABDELHAK E A,GWENOLA G,FRANC$OISE H,IYANC$.Involvement of polyamines in the interactingeffects of lowtemperature and mineral supply on Pringlea antiscorbutica(Kergue-len cabbage)seedlings[J].Journal of Experimental Botany,2004,55(399):1125-1134.
- [5]LIN Wen-xiong,WU Xing-hun,LIANG Kang-jing,GUO Yu-chun,HE Hua-qin,CHEN Fang-yu,LIANG Yi-yuan.Effect ofenhanced UV-B radiation on polyamine metabolism and endogenoushormone contents in rice(Oryza sativa L.)[J].Chinese Journal of Ap-plied Ecology,2002,13(7):807-813.林文雄,吴杏春,梁康迳,郭玉春,何华勤,陈芳育,梁义元.UV-B辐射增强对水稻多胺代谢及内源激素含量的影响[J].应用生态学报,2002,13(7):807-813.
- [6]TERESA C,LUDOVIC B,PAUL C.Modulation of the polyaminebiosynthetic pathway in transgenic rice confers tolerance to droughtstress[J].PNAS,2004,101(26):9909-9914.
- [7]GE C,CUI X,WANG Y,HU Y,FU Z,ZHANG D,CHENG Z,LIJ.BUD2,encoding an S-adenosylmethionine decarboxylase,is re-quired for Arabidopsis growth and development[J].Cell Research,2006,16:446-456.
- [8]GUO Zi-juan,SUN Feng-guo,ZHANG Shen-hao.The studies ofpolyaminesin friuts[J].Journal of Hebei Agricultural Sciences,2005,9(3):99-102.郭紫娟,孙风国,张慎好.多胺对果树生长发育的影响研究进展[J].河北农业科学,2005,9(3):99-102.
- [9]WANG Xiao-ling,XU Ji-zhong,SHAO Jian-zhu.Effects of exoge-nous spermidine on endogenous polyamines in leaves of Red Fujiapple and starkrimson in flower bud physiological differentiation pe-riod[J].Journal of Agricultural Universityof Hebei,2005,28(6):32-35.王晓玲,徐继忠,邵建柱.苹果花芽生理分化期外源亚精胺对叶片内源多胺含量的影响[J].河北农业大学学报,2005,28(6):32-35.
- [10]XU Ji-zhong,CHEN Hai-jiang,MA Bao-kun,ZHANG Wen-cai.Effects of exogenous Spermidine on the levels of endogenous hor-mones and polyamines in the flowers and fruitlets of red Fuji apple[J].Acta Horticulturae Sinica,2001,28(3):206-210.徐继忠,陈海江,马宝焜,章文才.外源多胺对富士苹果花和幼果内源多胺与激素的影响[J].园艺学报,2001,28(3):206-210.
- [11]XU Ji-zhong,CHEN Hai-jiang,SHAO Jian-zhu,YUAN Xiao-luan,MA Bao-kun,ZHANG Wen-cai.Effects of exogenous polyamineson flower bud formation of red fuji apple variety[J].Journal of Fruit Science,1998,15(1):10-12.徐继忠,陈海江,邵建柱,袁小乱,马宝焜,章文才.外源多胺促进红富士苹果花芽形成的效应[J].果树学报,1998,15(1):10-12.
- [12]YANGJun-yu,LIUShu-xiang.Observation on the morphologyof vamycorrhizas in apple trees[J].Journal of Hebei Forestry College,Supp,1996,10:198-201.杨军玉,刘淑香.果树抗冻研究现状[J].河北林学院学报(增刊),1996,10:198-201.
- [13]LIU Jun,JI Xiao-jia,LIU You-liang.High performance liquidchromatography method for measuring polyamine content in planttissue[J].Plant Physiol Commun,2002,38(6):596-598.刘俊,吉晓佳,刘友良.检测植物组织中多胺含量的高效液相色谱法[J].植物生理学通讯,2002,38(6):596-598.
- [14]ZHAOShi-jie,XUChang-cheng,ZHOUQi,MENGQing-wei.Themend ofmenstruated MDAin plant tissue[J].Plant Physiol Commun,1994,30(3):207-210.赵世杰,许长成,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
- [15]SHEN W Y,KAZUYOSHI N,SHOJI T.Involvement of polyaminesinthechillingtoleranceofcucumbercultivars[J].Plant Physiol,2000,124:431-440.
- [16]ZHOU Yu-ping,WANG Zheng-xun,TIAN Chang-en.Studies onthe relation of polyamines and cold resistance of banana[J].Guihaia,2003,23(4):252-256.周玉萍,王正询,田长恩.多胺与香蕉抗寒性的关系的研究[J].广西植物,2003,23(4):252-256.
- [17]KUSHAD M M,YELENOSKY G.Evalution of polyamine and pro-linelevelsduringlowtemperatureacclimationof Citrus[J].Plant Phys-iol,1987,84(3):692-695.
- [18]GUYE M G,VIGH L,WILSON J M.Polyamine titre in relatian tochill-sensitivity in Phaseolus sp[J].Journal of Experimental Botany,1986,37:1036-1043.
- [19]MCDONALD R E,KUSHAD MM.Accumulation of putrescine dur-ingchillinginjuryoffruit[J].Plant Physiol,1986,82(1):324-326.
- [20]SERRANO M,MARTINEZ-MADRID MC,PRETE MT.FERNAN-DOR,FELIXR.Modified atmosphere packagingminimizes increas-es in putrescine and abscisic acid levels caused by chilling injury inpepper fruit[J].Journal of Agriculture and Food Chemistry,1997,45:1668-1672.
- [21]FAN Hua,FENG Shuang-qing,ZHAO Yu-mei.The Correlation of Polyamines with chilling injury of cucumber and tomato and thetreatments for alleviatingchillinginjury[J].Journal of China Agricul-tural University,1996,1(1):108-111.范华,冯双庆,赵玉梅.黄瓜、番茄冷害以及黄瓜温度预处理与多胺的相关性[J].中国农业大学学报,1996,1(1):108-111.
- [22]WANG Yong,LU Wang-jin,ZHANG Shao-qi,XIE Hui.ABA andputrescine treatments alleviate chillinginjuryin banana fruits duringstorage at 8℃[J].Acta Photophysiologica Sinica,2003,29(6):549-554.王勇,陆旺金,张昭其,谢会.ABA和腐胺处理减轻香蕉果实贮藏冷害[J].植物生理与分子生物学学报,2003,29(6):549-554.
- [23]YU Bing-jun,JI Xiao-jia,LIU Jun,LIU You-liang.Changes ofpolyamines level in Glycine soja and Glycine max seedlings under Na Cl stress[J].Chinese Journal of Applied Ecology,2004,15(7):1223-1226.於丙军,吉晓佳,刘俊,刘友良.氯化钠胁迫下野生和栽培大豆幼苗体内的多胺水平变化[J].应用生态学报,2004,15(7):1223-1226.
- [24]ZAPATA P J,SERRANO M,PRETEL MT,AMOROS A,BOTELLAM$.Polyamines and ethylene changes during germination of differ-ent plant species under salinity[J].Plant Science,2004,167:781-788.