冉春;张云飞;陈飞;刘浩强;李鸿筠;胡军华;姚廷山;

• 1:中国农业科学院柑桔研究所
• 2:国家柑桔工程技术研究中心
• 3:西南大学园艺园林学院
• 4:南方山地园艺学教育部重点实验室

摘要(Abstract)：

【目的】为了明确谷胱甘肽S-转移酶(GST)基因、羧酸酯酶(CarE)基因,过氧化氢酶(CAT)基因在柑橘全爪螨Panonychus citri抗性中的作用,【方法】在室内用噻螨酮对柑橘全爪螨进行抗性选育,进一步构建抗/敏品系数字基因表达谱,采用RPKM法对柑橘全爪螨敏感品系和噻螨酮抗性品系3种代谢抗性相关基因进行表达差异分析。【结果】经过20代抗性选育,获得了柑橘全爪螨噻螨酮抗性品系,与敏感品系比较,柑橘全爪螨对噻螨酮的抗性倍数达到3 532.12倍。基因差异性分析发现,抗性品系中有11条GST基因、17条CarE基因和6条CAT基因表达上调;14条GST基因、24条CarE基因和3条CAT基因表达下调。上调倍数最高的GST基因、CarE基因和CAT基因分别为Unigene31530[log2 ratio(RS/SS)=1.05]、Unigene23121[log2 ratio(RS/SS)=2.05]和Unigene31477[log2 ratio(RS/SS)=10.04]。进一步对Unigene31477进行荧光定量PCR分析发现,抗性和敏感品系基因表达水平没有显著差异。【结论】根据柑橘全爪螨抗/敏性品系基因表达差异推断,GST、CarE和CAT基因可能与柑橘全爪螨对噻螨酮产生的抗性没有密切关系。

关键词(KeyWords)： 柑橘全爪螨;噻螨酮;抗性品系;敏感品系;代谢酶;基因表达差异

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划课题(2012BAD19B06);; 长江学者和创新团队发展计划项目(IRT0976);; 柑桔学重庆市市级重点实验室开放基金计划资助项目

作者(Author): 冉春;张云飞;陈飞;刘浩强;李鸿筠;胡军华;姚廷山;

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