余丹丹;邵兴锋;许凤;王鸿飞;

• 1:宁波大学食品科学与工程系

摘要(Abstract)：

从原产澳大利亚的互叶白千层植物中提取的茶树精油,作为潜在的生物保鲜剂具有广谱的抑菌性能,可以在果实采后病原真菌病害控制上起积极作用,有效延长果实贮藏期。综述了茶树精油采前喷洒、采后浸渍、熏蒸及复合处理等多种运用于果实保鲜的处理方法,分析了茶树精油不同处理方式对果实品质、病害控制的作用效果及各自商业应用上的优缺点。阐明茶树精油的保鲜作用机制不仅表现在对采后病原真菌菌体形态、超微结构和细胞代谢水平的直接破坏作用,也可能间接的诱导提高果实的抗病防御能力。并进一步讨论茶树精油单组分及组分之间的相互作用,结合其在真菌亚细胞水平上的影响来阐述茶树精油抗真菌作用机制。

关键词(KeyWords)： 果实;茶树精油;真菌;机制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31371860、31271943);; 浙江省公益性应用研究项目(2013C32094);; 浙江省自然科学基金(LY12C20003);; 浙江省教育厅中青年学科带头人学术攀登项目(pd2013110);; 宁波市自然科学基金(2012A610143)

作者(Author): 余丹丹;邵兴锋;许凤;王鸿飞;

Email:

DOI:

参考文献(References)：

• [1]ISMAN M B.Plant essential oils for pest and disease management[J].Crop Protection,2000,19(8):603-608.
• [2]LANCIOTTI R,GIANOTTI A,PATRIGNANI F,BELLETTI N,GUERZONI M E,GARDINI F.Use of natural aroma compounds to improve shelf-life and safety of minimally processed fruits[J].Trends in Food Science&Technology,2004,15(3-4):201-208.
• [3]ZHOU Xiao-wei,WANG Jing,GU Nie.ZHENG Yong-hua,Research progress in preservative effect of plant essential oil on fruits and vegetables[J].Food Science,2010,31(21):427-430.周晓薇,王静,顾镍,郑永华.植物精油对果蔬防腐保鲜作用研究进展[J].食品科学,2010,31(21):427-430.
• [4]BAKKALI F,AVERBECK S,AVERBECK D,IDAOMAR M.Biological effects of essential oils–a review[J].Food and Chemical Toxicology,2008,46(2):446-475.
• [5]CHEN Jian-yan,LI Yong-yu,WU Shao-hua.Advances in mechanism of biological activities of plant essential oils[J].Natural Product Research and Development,2012,24(9):1312-1318.陈建烟,李永裕,吴少华.植物精油生物活性作用机理研究进展[J].天然产物研究与开发,2012,24(9):1312-1318.
• [6]HOLLEY R A,PATEL D.Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials[J].Food Microbiology,2005,22(4):273-292.
• [7]LACHOWICZ K,JONES G,BRIGGS D,BIENVENU F,WAN J,WILCOCK A,COVENTRY M.The synergistic preservative effects of the essential oils of sweet basil(Ocimum basilicum L.)against acidtolerant food microflora[J].Letters in Aapplied Microbiology,1998,26(3):209-214.
• [8]REDDY B,ANGERS P,GOSSELIN A,ARUL J.Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits[J].Phytochemistry,1998,47(8):1515-1520.
• [9]WU Peng-cang,ZHANG Wei.Research progress in Tea Tree Oil[J].China Pharmaceuticals,2009,18(3):61-63.吴鹏昌,张伟.茶树油的研究进展[J],中国药业,2009,18(3):61-63.
• [10]CHEN Hai-yan,ZHONG Chang-yong,HUANG Yao-heng.Present status and developing tendency of natural tea tree oil in China[J].Journal of Chemical Industry of Forest Products,2003,37(4):35-36.陈海燕,钟昌勇,黄耀恒,我国天然茶树油的研究现状及发展方向[J].林产化工通讯,2003,37(4):35-36.
• [11]CARSON C F,HAMMER K A,RILEY T V.Melaleuca alternifolia(Tea Tree)oil:a review of antimicrobial and other medicinal properties[J].Clinical Microbiology Reviews,2006,19(1):50-62.
• [12]SWORDS G,HUNTER G.Composition of Australian tea tree oil(Melaleuca alternifolia)[J].Journal of Agricultural and Food Chemistry,1978,26(3):734-737.
• [13]ZHONG Zhen-sheng,FAN Li-fei,HUANG Ji-bing.Chemical components and antimicrobial activity of tea tree oil from introduced Melaleuca alternifolia[J].Journal of South China University of Technology:Natural Science Edition,2011,39(1):53-57.钟振声,樊丽妃,黄继兵,引种互叶白千层茶树油的化学成分及抑菌活性术[J].华南理工大学学报:自然科学版,2011,39(1):53-57.
• [14]HAMMER K A,CARSON C F,RILEY T V,NIELSEN J B.A review of the toxicity of Melaleuca alternifolia(tea tree)oil[J].Food Chemical Toxicology,2006,44(5):616-625.
• [15]ANTUNES M D C,CAVACO A M.The use of essential oils for postharvest decay control.A review[J].Flavour and Fragrance Journal,2010,25(5):351-366.
• [16]WASHINGTON W,ENGLEITNER S,BOONTJES G,SHANMUGANATHAN N.Effect of fungicides,seaweed extracts,tea tree oil,and fungal agents on fruit rot and yield in strawberry[J].Animal Production Science,1999,39(4):487-494.
• [17]PONCE A,VALLE C D,ROURA S.Shelf life of leafy vegetables treated with natural essential oils[J].Journal of Food Science,2004,69(2):50-56.
• [18]BADAWY F I,SALLAM M N,IBRAHIM A,ASRAN M.Efficacy of some essential oils on controlling green mold of orange and their effects on postharvest quality parameters[J].Plant Pathology Journal,2011,10(4):168-174.
• [19]TRIPATHI P,DUBEY N.Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables[J].Postharvest Biology and Technology,2004,32(3):235-245.
• [20]AMIRI A,DUGAS R,PICHOT A L,BOMPEIX G.In vitro and in vitro activity of eugenol oil(Eugenia caryophylata)against four important postharvest apple pathogens[J].International Journal of Food Microbiology,2008,126(1):13-19.
• [21]HAN Zhi-ping,ZHU De-min,KUANG Yu.Preservation effect of tea tree oil on banana fruit[C].Chinese Institute of Tropica1 Crops,2007:223-226.韩志萍,朱德明,匡钰.茶树油对香蕉保鲜效果的初步研究[C].中国热带作物学会,2007:223-226.
• [22]BOSQUEZ-MOLINA E,JESUS E,BAUTISTA-BANOS S,VERDECALVO J,MORALES-LOPEZ J.Inhibitory effect of essential oils against Colletotrichum gloeosporioides and Rhizopus stolonifer in stored papaya fruit and their possible application in coatings[J].Postharvest Biology and Technology,2010,57(2):132-137.
• [23]ZHONG Ye-jun,XU Xin-yuan,LIU Cheng-mei,LIU Wei.Effect of tea tree oil,Eugenol and Citral on keeping quality of litchi[J].Jiangxi Food Industry,2011(4):42-45.钟业俊,徐欣源,刘成梅,刘伟.茶树油、丁香酚、柠檬醛在荔枝保鲜中的应用[J].江西食品工业,2011(4):42-45.
• [24]JING Wei,SU Zi-peng,ZHU De-ming,HAN Zhi-ping.Effects of tea tree oil fumigation on banana postharvest disease colletotrichum musae[J].Transactions of the CSAE,2011,27(5):378-384.静玮,苏子鹏,朱德明,韩志萍.茶树油熏蒸处理对香蕉采后炭疽病害的影响[J].农业工程学报,2011,27(5):378-384.
• [25]CHENG Sai,SHAO Xing-feng,GUO An-nan,SONG Yu-xing.Effects of tea tree oil fumigation on disease and quality of postharvest strawberry fruits[J].Transactions of the CSAE,2011,27(4):383-388.程赛,邵兴锋,郭安南,宋钰兴.茶树油熏蒸对草莓采后病害和品质的影响[J].农业工程学报,2011,27(4):383-388.
• [26]SOYLU E M,KURT S,SOYLU S.In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea[J].International Journal of Food Microbiology,2010,143(3):183-189.
• [27]LI Dian-xin,JIANG Lin-lin,ZHU Na,PAN Lei-qing,TU Kang.Research progress of the food preservation by plant essential oils[J].Science and Technology of Food Industry,2012,33(12):396-400.李殿鑫,江琳琳,朱娜,潘磊庆,屠康.植物精油在食品保鲜中的研究进展[J].食品工业科技,2012,33(12):396-400.
• [28]CHFER M,SNCHEZ-GONZLEZ L,GONZLEZ-MART NEZ C,CHIRALT A.Fungal decay and shelf life of oranges coated with chitosan and bergamot,thyme,and tea tree essential oils[J].Journal ofFood Science,2012,77(8):182-187.
• [29]NCHEZ-GONZLEZL L,GONZLEZ-MARTNEZ C,CHIRALTA,CHFER M.Physical and antimicrobial properties of chitosan-tea tree essential oil composite films[J].Journal of Food Engineering,2010,98(4):443-452.
• [30]MEZ-ESTACA J G,LPEZDELACEY A,LPEZ-CABALLERO M,GMEZ-GUILLN M,MONTERO P.Biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation[J].Food Microbiology,2010,27(7):889-896.
• [31]SERRANO M,MARTNEZ-ROMERO D,GUILLN F,VALVERDE J M,ZAPATA P J,CASTILLO S,VALERO D.The addition of essential oils to MAP as a tool to maintain the overall quality of fruits[J].Trends in Food Science&Technology,2008,19(9):464-471.
• [32]ZHANG Yan,KAN Jian-quan.Study and application of naturalChinese herbal medicine as fruitand vegetable antistaling agent[J].China Food Additives,2007(6):106-109.张艳,阚健全.中草药提取物在果蔬保鲜中的研究进展[J].中国食品添加剂,2007(6):106-109.
• [33]ZHU De-ming,DING Li,KUANG Yu,HAN Zhi-ping,LI Ji-hua.Study on the effect of tea tree oil on colletotrichum musae[J].Food Research and Development,2008,29(6):134-137.朱德明,丁丽,匡钰,韩志萍,李积华.茶树油对芭蕉炭疽菌菌丝生长和孢子萌发的抑制活性[J].食品研究与开发,2008,29(6):134-137.
• [34]SZCZERBANIK M,JOBLING J,MORRIS S,HOLFORD P.Essential oil vapours control some common postharvest fungal pathogens[J].Animal Production Science,2007,47(1):103-109.
• [35]TAO Feng-yun,ZHANG Xin-miao,YU Jun,MA Run-yu.Progress on mechanism of antimicrobial activity of tea tree oil[J].Chinese Journal of Antibiotics,2006,(31)5:261-266.陶凤云,张新妙,俞军,马润宇.茶树油抗菌作用机理研究进展[J].中国抗生素杂志,2006,(31)5:261-266.
• [36]KATHIRVEL P,RAVI S.Chemical composition of the essential oil from basil(Ocimum basilicum Linn.)and its in vitro cytotoxicity against HeLa and HEp-2 human cancer cell lines and NIH 3T3mouse embryonic fibroblasts[J].Natural Product Research,2012,26(12):1112-1118.
• [37]TERZI V,MORCIA C,FACCIOLI P,VALE G,TACCONI G,MALNATI M.In vitro antifungal activity of the tea tree(Melaleuca alternifolia)essential oil and its major components against plant pathogens[J].Letters in Applied Microbiology,2007,44(6):613-618.
• [38]HAMMER K A,CARSON C F,RILEY T V.Antifungal activity of the components of Melaleuca alternifolia(tea tree)oil[J].Journal of Applied Microbiology,2003,95(4):853-860.
• [39]COX S,MANN C,MARKHAM J.Interactions between components of the essential oil of Melaleuca alternifolia[J].Journal of Applied Microbiology,2001,91(3):492-497.
• [40]HAMMER K,CARSON C,RILEY T.In vitro activity of Melaleuca alternifolia(tea tree)oil against dermatophytes and other filamentous fungi[J].Journal of Antimicrobial Chemotherapy,2002,50(2):195-199.
• [41]SHAO X,CHENG S,WANG H,YU D,MUNGAI C.The possible mechanism of antifungal action of tea tree oil on Botrytis cinerea[J].Journal of Applied Microbiology,2013,114(6):1642-1649.
• [42]CARSON C F,MEE B J,RILEY T V.Mechanism of action of Melaleuca alternifolia(tea tree)oil on Staphylococcus aureus determined by time-kill,lysis,leakage,and salt tolerance assays and electron microscopy[J].Antimicrobial Agents and Chemotherapy,2002,46(6):1914-1920.
• [43]HAMMER K A,CARSON C F,RILEY T V.Antifungal effects of Melaleuca alternifolia(tea tree)oil and its components on Candida albicans,Candida glabrata and Saccharomyces cerevisiae[J].Journal of Antimicrobial Chemotherapy,2004,53(6):1081-1085.
• [44]ESCALANTE A,GATTUSO M,PREZP,ZACCHINO S.Evidence for the mechanism of action of the antifungal phytolaccoside B isolated from Phytolacca tetramera Hauman[J].Journal of Natural Products,2008,71(10):1720-1725.
• [45]TIAN J,BAN X,ZENG H,HE J,CHEN Y,WANG Y.The mechanism of antifungal action of essential oil from dill(Anethum graveolens L.)on Aspergillus flavus[J].Public Library of Science One,2012,7(1):e30147.
• [46]COX S,GUSTAFSON J,MANN C,MARKHAM J,LIEW Y,HARTLAND R,BELL H,WARMINGTON J,WYLLIE S.Tea tree oil causes K+leakage and inhibits respiration in Escherichia coli[J].Letters in Applied Microbiology,1998,26(5):355-358.
• [47]JIN Er-yong.The antifungal activities of BIT of B.cinerea and the preliminary studies on the mechanism[D].Xi’an:Northwest University,2008.荆二勇.BIT对灰霉菌抑菌活性及抑菌机制的初步研究[D].西安:西北大学,2008.
• [48]QIU J,ZHANG X,LUO M,LI H,DONG J,WANG J,LENG B,WANG X,FENG H,REN W.Subinhibitory concentrations of perilla oil affect the expression of secreted virulence factor genes in Staphylococcus aureus[J].Public Library of Science one,2011,6(1):e16160.
• [49]WU Fang-li.Primary study on inhibiting mechanism of propamidine against Botrytis Cinerea[D].Yangling:Northwest Agriculture&Forestry University,2008.吴方丽.丙烷脒对番茄灰霉病菌的抑菌机理初探[D].杨凌:西北农林科技大学,2008.
• [50]ZHANG Z,TIAN S,ZHU Z,XU Y,QIN G.Effects of 1-methylcyclopropene(1-MCP)on ripening and resistance of jujube(Zizyphus jujuba cv.Huping)fruit against postharvest disease[J].LWT-Food Science and Technology,2012,45(1):13-19.
• [51]NISHIKAWA F,KATO M,HYODO H,IKOMA Y,SUGIURA M,YANO M.Ascorbate metabolism in harvested broccoli[J].Journal of Experimental Botany,2003,54(392):2439-2448.
• [52]DIXON R A,ACHNINE L,KOTA P,LIU C J,REDDY M,WANG L.The phenylpropanoid pathway and plant defence-a genomics perspective[J].Molecular Plant Pathology,2002,3(5):371-390.
• [53]VAN LOON L,REP M,PIETERSE C.Significance of inducible defense-related proteins in infected plants[J].Annuul Reviews Phytopathol,2006,44:135-162.
• [54]SHAO X,WANG H,XU F,CHENG S.Effects and possible mechanisms of tea tree oil vapor treatment on the main disease in postharvest strawberry fruit[J].Postharvest Biology and Technology,2013,77:94-101.
• [55]ARREBOLA E,SIVAKUMAR D,BACIGALUPO R,KORSTEN L.Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases[J].Crop Protection,2010,29(4):369-377.