赵莘;朱自果;徐炎;张朝红;王跃进;

• 1:西北农林科技大学园艺学院农业部西北园艺植物种质资源利用重点开放实验室陕西省农业分子生物学重点实验室

摘要(Abstract)：

根据从本课题组前期构建的中国野生葡萄华东葡萄株系白河-35-1在白粉病诱导下的cDNA文库中获得的1条与抗白粉病相关的EST序列,设计特异引物,结合RACE(Rapid-amplrfication of cDNA ends)技术克隆了中国野生华东葡萄白河-35-1广泛逆境蛋白基因(Universal stress protein,USP)全长序列。该基因全长836 bp,编码175个氨基酸,GenBank登录号为GU946975。进一步研究该基因在白粉菌诱导下的相对表达水平,我们通过半定量RT-PCR技术,研究了VpUSP在中国野生华东葡萄抗病、感病株系中的表达变化,结果表明,广泛逆境蛋白在中国野生华东葡萄感病株系中,接种白粉病前后均有表达,但表达量变化不明显;然而,抗病的中国野生华东葡萄株系在接种白粉病后VpUSP基因表达量呈明显的增加趋势,初步表明中国野生华东葡萄VpUSP基因在与白粉病菌互作过程中具有表达活性。

关键词(KeyWords)： 中国野生葡萄;RACE(Rapid-amplrficationofcDNAends);葡萄白粉病

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金((30971972);; 现代农业产业技术体系专项(Nycytx-30-ZP-06);; 公益性行业(农业)科研专项经费子课(200903044)

作者(Author): 赵莘;朱自果;徐炎;张朝红;王跃进;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.01.024

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