利用RAPD标记评价桃种间杂交一代群体的分离方式Evaluation of Segregation Patterns of Peach in an Interspecific F1 Hybrid with RAPD Markers
乔飞,王力荣,范崇辉,朱更瑞,方伟超
摘要(Abstract):
以毛桃2-7(Prunus persica)、山桃白山碧桃(Prunus davidiana)以及两者的F_1代为试材,对RAPD标记的分离方式进行分析。结果表明,RAPD标记在F_1代呈3种分离方式:孟德尔分离、偏离孟德尔分离规律、异常分离。3种分离位点出现的频率和数量分别为:80%、240,14%、42,6%、18。呈孟德尔分离的情况有:不分离、1:1分离和3:1分离,其中不分离的频率为64%。并对偏离孟德尔分离比例和异常分离的RAPD标记进行分析。其研究结果可为该群体是否适合构建遗传连锁图谱进行评价及进一步利用该群体奠定良好基础。
关键词(KeyWords): 桃;RAPD标记;分离方式;多态性
基金项目(Foundation):
作者(Author): 乔飞,王力荣,范崇辉,朱更瑞,方伟超
Email:
DOI: 10.13925/j.cnki.gsxb.2003.04.017
参考文献(References):
- [1] Baird WV, Estager AS, Well J. Estimating nuclear DNA content in peach and related diploid species using laser flow egtometry and DNA hybridisation[J]. J Amer Soc Hort Sci, 1994, 119: 1 312~1 316.
- [2] Hemmat M, Weeden N F, Manganaris AG,et al. Molecular marker linkage map for apple[J]. J Hered, 1994, 85(1) : 4~11.
- [3] 刘孟军, Shin Yong-UK,Yae Byeong-Woo. RAPD标记在苹果种间杂交一代的分离方式[J].园艺学报, 1998, 25(3) : 214~219.
- [4] Ayliffe MA, Lawrence GJ, Ellis JG, et al. Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands[J].Nucleic Acid Research, 1994, 22(9) :1 632~1 636.
- [5] 房经贵,章镇,马正强,等.AFLP标记在两个芒果品种间杂交F_1代的多态性及分离方式[J].中国农业科学,2000,33(3) :19~24.
- [6] 房经贵,章镇,周兰华,等.RAPD标记鉴定果树芽变的可行性分析[J].果树学报,2001,18(3) :182~185.
- [7] Stockinger EJ, Mulimx CM, Long TS, et al. A Linkage map of sweet cherry based on RAPD analysis of a microspore,derived callus culture population[J]. J Hered, 1996, 87:214~218.
- [8] Dalbo MA, Ye GN, Weeden NF, et al. A gene controlling sex in grapevines placed on a molecular marker based genetic map[J]. Genome, 2000, 43(2) : 333~340.