张倩;曹珂;朱更瑞;方伟超;王新卫;王力荣;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】探索扩张蛋白(EXPANSIN)基因家族在桃抗南方根结线虫过程中的作用。【方法】首先对桃基因组中的EXPANSIN基因家族进行生物信息学分析,然后利用转录组测序对EXPANSIN基因在南方根结线虫侵染免疫型(‘红根甘肃桃1号’)和高感型(‘贝蕾’)桃种质根系后的不同时期的表达丰度进行分析。【结果】桃EXPANSIN基因家族有27个成员,这些成员分布在8条染色体上,以第2和第5号染色体上最多,均为5个。基因结构分析显示,这些基因包含1⁵个外显子,大部分EXPANSIN基因包含DPBB_1和Pollen_allerg_1保守结构域,聚类分析将这27个成员分为2大类,其中有8个与拟南芥中已注释的与线虫侵染过程中巨细胞形成有关的蛋白质聚在一起,均属于EXPA亚类。转录组分析表明,这8个EXPANSIN基因在对南方根结线虫免疫和高感2种种质不同时期的表达趋势存在明显差异,其中ppa010314m、ppa010226m在‘贝蕾’中表达量随侵染上调,而在‘红根甘肃桃1号’中上调不明显,这2个基因可能是参与南方根结线虫侵染过程中巨细胞形成的关键基因。【结论】桃EXPANSIN家族成员在结构上高度保守,其中多个成员可能参与调控桃抗南方根结线虫过程。

关键词(KeyWords)： 桃;EXPANSIN基因家族;南方根结线虫;抗性

Abstract:

Keywords:

基金项目(Foundation): 中央级科研院所基本科研业务费(Y2017JC37);; 中国农业科学院科技创新工程项目[CAAS-ASTIP-2017-ZFRI-(01)]

作者(Authors): 张倩;曹珂;朱更瑞;方伟超;王新卫;王力荣;

DOI: 10.13925/j.cnki.gsxb.20170259

参考文献(References)：

• [1]牛俊海,郭仰东,简恒.RNA干扰在植物抗根结线虫病基因工程应用中的研究进展[J].基因组学与应用生物学,2009,28(1):167-173.NIU Junhai,GUO Yangdong,JIAN Heng.Progress in the Study ofRNAi mediated plant root-knot nematodes resistant genetic engi-neering[J].Genomics and Applied Biology,2009,28(1):167-173.
• [2]TRUDGILL D L,BLOK V C.Apomictic,polyphagous root-knotnematodes:Exceptionally successful and damaging biotrophicroot pathogens[J].Annual Review of Phytopathology,2001,39(39):53-77.
• [3]FORNECK A,HUBER L.(A)sexual reproduction-a review of lifecycles of grape phylloxera,Daktulosphaira vitifoliae[J].Entomolo-gia Experimentalis et Applicata,2009,131(1):1-10.
• [4]KELLOW A V,SEDGLEY M,VAN HEESWIJCK R.Interactionbetween Vitis vinifera and grape phylloxera:changes in root tissueduring nodosity formation[J].Annals of Botany,2004,93(5):581-590.
• [5]COSGROVE D J.Loosening of plant cell walls by expansins[J].Nature,2000,407(6802):321-326.
• [6]DAL S S,VANNOZZI A,TORNIELLI G B,FASOLI M,VENTURINI L,PEZZOTTI M,ZENONI S.Genome-wide analy-sis of the expansin gene superfamily reveals grapevine-specificstructural and functional characteristics[J].PLo S One,2013,8(4):2206.
• [7]KENDE H,BRADFORD K,BRUMMELL D,CHO H T,COSGROVE D,FLEMING A,GEHRING C,LEE Y.Nomencla-ture for members of the expansin superfamily of genes and pro-teins[J].Plant Molecular Biology,2004,55(3):311-314.
• [8]LI L C,COSGROVE D J.Grass group I pollen allergens(beta-expansins)lack proteinase activity and do not cause wall loosen-ing via proteolysis[J].European Journal of Biochemistry,2001,268(15):4217-4226.
• [9]BRUMMELL D A,HARPSTER M H.Cell wall metabolism infruit softening and quality and its manipulation in transgenicplants[J].Plant Molecular Biology,2001,47(1/2):311-339.
• [10]SAMPEDRO J,DANIEL J C.The expansin superfamily[J].Ge-nome Biology,2005,6(12):242.
• [11]CADERAS D,MUSTER M,VOGEL H,MANDEL T,ROSE J K.Limited correlation between expansin gene expression and elonga-tion growth rate[J].Plant Physiology,2000,123(4):1399-1413.
• [12]CHO H T,COSGROVE D J.Regulation of root hair initiation andexpansin gene expression in Arabidopsis[J].Plant Cell,2002,14(12):3237-3253.
• [13]CHO H T,COSGROVE D J.Expansins as agents of hormone ac-tion[M].Dordrecht:Plant Hormones,2004:262-281.
• [14]SANCHEZ A M,MATEOS I,LABRADOR E,DOPICO B.Brassi-nolides and IAA induce the transcription of four a-expansingenes related to development in Cicer arietinum[J].Plant Physiolo-gy Biochemistry,2004,42(9):709-716.
• [15]WU Y,MEELEY R B,COSGROVE D J.Analysis and expressionof the a-expansins and b-expansins gene families in maize[J].Plant Physiology,2001,126(1):222-232.
• [16]BALESTRINI R,COSGROVE D J,BONFANTE P.Differential lo-cation of a-expansin proteins during the accommodation of rootcells to an arbuscular mycorrhizal fungus[J].Planta,2004,220(6):889-899.
• [17]GIORDANO W,HIRSCH A M.The expression of Ma EXP1,a Melilotus alba expansin gene,is upregulated during the sweet-clo-ver-Sinorhizobium meliloti interaction[J].Molecular Plant-mi-crobe Interactions,2004,17(6):613-622.
• [18]GAL T,AUSSENBERG E,BURDMAN S,KAPULNIK Y,KOL-TAI H.Expression of a plant expansin is involved in the establish-ment of root knot nematode parasitism in tomato[J].Planta,2006,224(1):155-162.
• [19]GRIESSER M,GRUNDLER F M W.Quantification of tomato ex-pansins in nematode feeding sites of cyst and root-knot nematodes[J].Journal of Plant Diseases and Protection,2008,115(6):263-272.
• [20]JAMMES F,LECOMTE P,DE ALMEIDAl ENGLER J,BITTONF,MARTIN MAGNIETTE M L,RENOU J P,ABAD P,FAVERYB.Genome-wide expression profiling of the host response to root-knot nematode infection in Arabidopsis[J].The Plant Journal,2005,44(3):447-458.
• [21]WIECZOREK K,GOLECKI B,GERDES L,HEINEN P,SZAKA-SITS D.Expansins are involved in the formation of nematode-in-duced syncytia in roots of Arabidopsis thaliana[J].The Plant Jour-nal,2006,48(1):98-112.
• [22]HEBA I M M,HOSSEINI P,ALKHAROUF N W,EBTISSAM HH A,ABD EL K G E Y.Analysis of gene expression in soybean(Glycine max)roots in response to the root knot nematode Meloidogyne incognita using microarrays and KEGG pathways[J].BCM Genomics,2011,12(1):220.
• [23]TIRUMALARAJU S V,JAIN M,GALLO M.Differential gene ex-pression in roots of nematode-resistant and susceptible peanut(Arachis hypogaea)cultivars in response to early stages of peanutroot-knot nematode(Meloidogyne arenaria)parasitization[J].Journal of Plant Physiology,2011,168(5):481-492.
• [24]PERE A,IGNAZIO V,BRYON S,TATYANA Z,ALBERT A.The peach genome[J].Tree Genetics&Genomes,2012,8(3):531-547.
• [25]VERDE I,ABBOTT A G,SCALABRIN S,JUNG S,SHU S.Thehigh-quality draft genome of peach(Prunus persica)identifiesunique patterns of genetic diversity,domestication and genomeevolution[J].Nature Genetics,2013,45(5):487-494.
• [26]HAYAMA H,ITO A,MORIGUCHI T,KASHIMURA Y.Identifi-cation of a new expansin gene closely associated with peach fruitsoftening[J].Postharvest Biology&Technology,2003,29(1):1-
• [27]TAMURA K,DUDLEY J,NEI M,KUMAR S.MEGA4:molecular evolutionary genetics analysis(MEGA)software version 4.0[J].Molecular Biology and Evolution,2007,24(8):1596-1599.
• [28]李海炎,王力荣,曹珂,朱更瑞,王新卫,方伟超,陈昌文,王小丽.桃根系与南方根结线虫早期互作的组织病理学研究[J].园艺学报,2015,42(6):1040-1048.LI Haiyan,WANG Lirong,CAO Ke,ZHU Gengrui,WANG Xin-wei,FANG Weichao,CHEN Changwen,WANG Xiaoli.Histo-pathological of the early interactions between peach roots and Meloidogyne incognita[J].Acta Horticulturae Sinica,2015,42(6):1040-1048.
• [29]SHANI Z,DEKEL M,ROIZ L,HOROWITZ M,KOLOSOVSKI N,LAPIDOT S,ALKAN S.Expression of endo-1,4-beta-glucanase(cel1)in Arabidopsis thaliana is associated with plant growth,xy-lem development and cell wall thickening[J].Plant Cell Reports,2006,25(10):1067-1074.
• [30]DAVIS E L,HUSSEY R S,BAUM T J.Getting to the roots of para-sitism by nematodes[J].Trends Parasitol,2004,20(3):134-141.
• [31]GOVERSE A,OVERMARS H,ENGELBERTINK J,SCHOTS A.Both induction and morphogenesis of cyst nematode feeding cellsare mediated by auxin[J].Molecular Plant-microbe Interactions,2000,13(10):1121-1129.
• [32]SWARUP K,BENKOVA E,SWARUP R,CASIMIRO I,PERETB,YANG Y.The auxin influx carrier LAX3 promotes lateral rootemergence[J].Nature Cell Biology,2008,10(8):946-954.
• [33]GENING M L,TSVETKOV Y E,PIER G B,NIFANTIEV N E.Synthesis ofβ-(1->6)-linked glucosamine oligosaccharides cor-responding to fragments of the bacterial surface polysaccharidepoly-N-acetylglucosamine[J].Carbohydr Research,2007,342(3/4):567-575.
• [34]SAKAKIBARA H,TAKEI K,HIROSE N.Interactions between ni-trogen and cytokinin in the regulation of metabolism and develop-ment[J].Trends Plant Science,2006,11(9):440-448.
• [35]NEMHAUSER J L,HONG F,CHROY J.Different plant hor-mones regulate similar processes through largely nonoverlappingtranscriptional responses[J].Cell,2006,126(3):467-475.
• [36]MANTIRI F R,KURDYUKOV S,LOHAR D P,SHAROPOVA N,SAEED N A,WANG X D,VANDENBOSCH K A,ROSE R J.The transcription factor Mt SERF1 of the ERF subfamily identi-fied by transcriptional profiling is required for somatic embryogen-esis induced by auxin plus cytokinin in Medicago truncatula[J].Plant Physiology,2008,146(4):1622-1636.
• [37]SEKI Y,KIKUCHI Y,YOSHIMOTO R,ABURAI K,KANAI Y,RUIKE T,IWABATA K.Promotion of crystalline cellulose degra-dation by expansins from Oryza sativa[J].Planta,2015,241(1):83-93.
• [38]MOCKAITIS K,ESTELLE M.Auxin receptors and plant develop-ment:a new signaling paradigm[J].Annual Review Cell Develop-ment Biology,2008,24(24):55-80.
• [39]CATALA C,ROSE K C,BERNETT A B.Auxin-regulated genesencoding cell wall-modefying proteins are expressed during earlytomato fruit growth[J].Plant Physiology,2000,122(2):527-534.
• [40]KARCZMAREK A,OVERMARS H,HELDER J.Feeding cell de-velopment by cyst and root-knot nematodes involves a similar ear-ly,local and transient activation of a specific auxin-inducible pro-moter element[J].Molecular Plant Pathology,2004,5(4):343-346.