程和禾;吴雅琴;吴永杰;李玉生;陈龙;周锡明;赵艳华;

• 1:河北省农林科学院昌黎果树研究所

摘要(Abstract)：

【目的】为了研究影响杂交后代胚挽救萌发率的主要因素,并对其无核性状进行早期鉴定,【方法】以无核、抗病葡萄品种‘奥迪亚’为母本,‘玫瑰香’、‘摩尔多瓦’和‘红地球’3个主栽品种为父本进行杂交,通过L9(33)正交试验,建立简单高效的胚挽救体系,并用分子标记对杂交苗无核性状进行鉴定。【结果】结果表明,影响胚挽救萌发率的最关键因素为接种时期,其次为培养基种类和NAA浓度;以‘奥迪亚’为母本的杂交胚其适宜接种程序为授粉后49～56 d剥取胚珠,接种在B5或NN69培养基上充分发育70 d,再转入1/2 B5培养基上萌芽,‘奥迪亚’ב摩尔多瓦’杂交胚通过此程序萌发率达到了50.48%;经分子标记鉴定,各组合后代的无核株系均超过50%。【结论】利用胚挽救方法以‘奥迪亚’为杂交母本可高效创新葡萄无核种质。

关键词(KeyWords)： 葡萄;‘奥迪亚’;无核;胚挽救;分子鉴定

Abstract:

Keywords:

基金项目(Foundation): 河北省农林科学院项目(A09020203)

作者(Authors): 程和禾;吴雅琴;吴永杰;李玉生;陈龙;周锡明;赵艳华;

DOI: 10.13925/j.cnki.gsxb.2012.05.015

参考文献(References)：

• [1]LIU San-jun,KUAI Chuan-hua,YU Qiao-li,CHEN Yong-peng.Research and application of the inheritance of Seedlessness andembryo rescue techniques in seedless grape breeding[J].Journal ofFruit Science,2009,26(1):71-76.刘三军,蒯传化,于巧丽,陈勇鹏.葡萄无核性状遗传与胚挽救技术的研究及应用[J].果树学报,2009,26(1):71-76.
• [2]RAMMING D W.The use of embryo culture in fruit breeding[J].HortSci,1990,25(4):393-398.
• [3]ZHAO Yan-hua,WU Ya-qin,ZHAO Sheng-jian,GUO Zi-juan,CHENG He-he.Study on the embryo rescue of the crossed betweendiploid and tetraploid grape[J].Acta Agirculturae Boreali-Sinica,2005,20(6):110.赵艳华,吴雅琴,赵胜建,郭紫娟,程和禾.二倍体与四倍体葡萄品种间杂交胚抢救研究[J].华北农学报,2005,20(6):110.
• [4]CHENG He-he,WU Ya-qin,ZHAO Yan-hua,WU Yong-jie,LIYu-sheng,ZHAO Sheng-jian.Studies on embryo rescue techniquesof otilia seedless grape[J].Journal of Hebei Agricultural Sciences,2008,12(11):30-32.程和禾,吴雅琴,赵艳华,吴永杰,李玉生,赵胜建.奥迪亚无核葡萄胚抢救技术研究[J].河北农业科学,2008,12(11):30-32.
• [5]XU Hai-ying,YAN Ai-ling,ZHANG Guo-jun.Determination of theproper sampling period for embryo rescue from crosses betweendiploid and tetraploid grape cultivars[J].Scientia Agricultura Sinica,2005,38(3):629-633.徐海英,闫爱玲,张国军.葡萄二倍体与四倍体品种间杂交胚挽救取样时期的确定[J].中国农业科学,2005,38(3):629-633.
• [6]GUO Xiu-wu,GUO Yin-shan,ZHANG Hai-e,LI Yi-hui,LICheng-xiang.Effects of culture medium and inoculating date on em-bryo rescue of seedless grape[J].Acta Horticulturae Sinic,2007,34(2):329-332.郭修武,郭印山,张海娥,李轶晖,李成祥.接种时期和培养基对无核葡萄胚挽救的影响[J].园艺学报,2007,34(2):329-332.
• [7]WANG Yue-jin,YANG Ying-jun,ZHOU Peng,ZHANG Jian-xia,WANG Xi-ping.Detecting the seedless characteristics of the grapesin China with DNA probe[J].Acta Horticulturae Sinica,2002,29(2):105-108.王跃进,杨英军,周鹏,张剑霞,王西平.用DNA探针检测我国栽培的无核葡萄及辅助育种初探[J].园艺学报,2002,29(2):105-108.
• [8]ZHAO Mi-zhen,SU Jia-le,QIAN Ya-ming,WANG Zhuang-wei,DIAO Man-ni.Technolgy of ovule culture for Ruby Seedless grapecultivar[J].Journal of Fruit Science,2005,22(2):166-168.赵密珍,苏家乐,钱亚明,王状伟,刁嫚妮.红宝石无核葡萄胚珠培养成苗技术研究[J].果树学报,2005,22(2):166-168.
• [9]QI Gui-mei.Several factors affecting hybrid ovule culture and germi-nate of seedless grape[J].Sino-overseas Grapevine&Wine,2001(4):24-27.亓桂梅.无核葡萄杂种胚珠的培养及萌发成苗的若干影响因素[J].中国葡萄与葡萄酒,2001(4):24-27.
• [10]WANG Fei,WANG Yue-jin,ZHOU Hui-ling,WAN Yi-zhen,YANG Jin-xiao.Cytological study of embryo development and abor-tion in hybrid progeny of seedless grape and Chinese wild grapes[J].Jour of Northwest Sci-Tech Univ of Agri and For:Nat Sci Ed,2005,33(3):61-65.王飞,王跃进,周会玲,万怡震,杨进孝.无核葡萄与中国野生葡萄杂种胚发育和败育的细胞学研究[J].西北农林科技大学学报:自然科学版,2005,33(3):61-65.