牛茹萱;张剑侠;王跃进;翟焕;赵凯;

• 1:西北农林科技大学园艺学院·旱区作物逆境生物学国家重点实验室

摘要(Abstract)：

【目的】为了培育抗病抗寒无核葡萄新品种,【方法】以2个种子败育型无核品种‘波尔莱特’、‘红无籽露’作母本,以抗病抗寒的中国野生山葡萄株系‘黑龙江实生’、‘双优’及欧山杂种‘北醇’、‘00-1-10’(‘玫瑰香’ב黑龙江实生’)分别作父本杂交,授粉51 d后将胚珠分别接种于ER和MM4培养基上进行胚挽救,培养60 d后在WPM+BA 0.2 mg.L-1培养基上诱导成苗。【结果】结果表明,2个母本品种胚挽救的适宜培养基不同,‘波尔莱特’作母本适合于ER培养基,‘红无籽露’作母本适合于MM4培养基。2个母本基因型对胚珠的发育率和成苗率的影响差异不大,‘波尔莱特’略优于‘红无籽露’;但4个父本基因型对胚挽救效果影响较大,以欧山杂种‘00-1-10’和‘北醇’作父本的杂交组合胚株的发育率和成苗率明显高于山葡萄‘黑龙江实生’、‘双优’作父本的杂交组合。共获得无核葡萄胚挽救新种质50个株系。【结论】在胚挽救过程中,不同基因型的无核葡萄适宜于不同的基本培养基,欧山杂种比山葡萄更适宜于作杂交的父本。

关键词(KeyWords)： 无核葡萄;抗病;抗寒;胚挽救

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系建设专项基金(CARS-30-yz-7);; 陕西省科技攻关项目(2011K02-10)

作者(Authors): 牛茹萱;张剑侠;王跃进;翟焕;赵凯;

DOI: 10.13925/j.cnki.gsxb.2012.05.023

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