汪良驹;王中华;李志强;朱云娜;

• 1:南京农业大学园艺学院
• 2:南京农业大学园艺学院
• 3:南京农业大学园艺学院
• 4:南京农业大学园艺学院 南京210095

摘要(Abstract)：

为了探索更为经济适用的果实增色技术,以13年生富士苹果为材料,在果实着色初期(9月中旬)用200～800mg/L谷氨酸(Glu)溶液处理,然后分批取样,分析结果表明,不同浓度谷氨酸处理均能明显促进果皮花青素积累,增加果实着色面积,并明显增加果实可溶性糖含量。分析果实中内源5-氨基乙酰丙酸(ALA)含量,发现Glu能够促进内源ALA含量提高,推测Glu促进苹果着色可能与其促进ALA生物合成有关。分析果实着色过程中超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性,没有观察到这些抗氧化酶活性与果实着色之间存在着明显的相关关系。

关键词(KeyWords)： 富士苹果;L-谷氨酸;花青素;5-氨基乙酰丙酸(ALA);抗氧化酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30471181)。

作者(Author): 汪良驹;王中华;李志强;朱云娜;

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DOI:

参考文献(References)：

• [1]SAURE M C.External control of anthocyanin formation in apple[J].Hort Sci,1990,42:181￣218.
• [2]BAE R N,LEE S K.Influence of chlorophyll,internal ethylene,andPAL on anthocyanin synthesis in Fuji apple[J].J Korean Soc Hort Sci,1995,36(3):361～370.
• [3]ZHAO Z L,LI B G,QI G H,et al(赵志磊,李保国,齐国辉,等).Advances of studyon bagging effect on‘Fuji’s fruit quality[J].Hebei J ForestryOrchard Research(河北林果研究),2003,18(1):81￣86.(in Chinese)
• [4]CHALMERS D J,ARAGHER J P.Regulation of anthocyanin syn-thesisinappleskin.I.Involvementofethylene[J].AustJPlantPhysiol,1977(4):123￣131.
• [5]WANGLJ,WANGZH,LI ZQ,et al(汪良驹,王中华,李志强,等).Effect of5-Aminolevulinic acid on enhancing apple fruit col-oration[J].Journal of Fruit Science(果树学报),2004,21(6):512～515.(in Chinese)
• [6]WANGZH,WANGLJ,LI ZQ,et al(王中华,汪良驹,李志强,等).Promotion of5-aminolevulinic acid and genistein on antho-cyanin accumulation in apples[J].Acta Horticulturae Sinica(园艺学报),2006,33,in press.(in Chinese)
• [7]WANGLJ,JIANGWB,ZHANGZ,et al(汪良驹,姜卫兵,章镇,等).Biosynthesis and physiological activities of5-aminolevulinic acid and its potential application in agriculture[J].Plant Physiology Communications(植物生理学通讯),2003,39(3):185￣192.(in Chinese)
• [8]TONG Y A,ZHOU H J(仝月澳,周厚基).The nutrition thesis of fruit tree(果树营养诊断法)[M].Beijing:Agriculture Press,1982:112￣113.(in Chinese)
• [9]ZHANG Z Q(张振清).The determination of soluble sugar in plant material.See:Handbook ofplant physiologyexperiment(植物生理学实验手册)[M].Shanghai:Shanghai Science and Technology Press,1985:135￣138.(in Chinese)
• [10]MA Z B,CHENG Y E(马志本,程玉娥).The methods about an-thocyanin content in apple skin[J].China Fruits(中国果树),1984(4):49～51.(in Chinese)
• [11]FENGR Y(冯瑞云).The extract methods of chlorophyll[J].J Jiangsu Agricultural College(江苏农学院学报),1985,6(3):53～54.(in Chinese)
• [12]HARELE,KLEINS.Light dependent formation ofδ-aminolevulin-ic acid in etiolated leaves of higher plants[J].Biochem Biophys Res Commun,1972,49:364～370.
• [13]LI H S(李合生).The Principle and Technology of Plant Physiology&Biochemistry(植物生理生化实验原理和技术)[M],Beijing:Higher Education Press,2000:67￣169.(in Chinese)
• [14]ASADAK.Chloroplasts:Formation of active oxygen and its scaveng-ing[J].Methods Enzymol,1984,105:422￣429.
• [15]WANGH C,HUANGXM,HUGB,et al(王惠聪,黄旭明,胡桂兵,等).Studies on the relationship between anthocyanin biosynthe-sis and related enzymes in litchi pericarp[J].Scientia Agricultura Sinica(中国农业科学),2004,37(12):2028￣2032.(in Chinese)
• [16]ZHANGH Y,WANGSG,MOQY,et al(张华云,王善广,牟其芸,等).Effect of bagging on the peel structure and PPO,POD activ-ityofPyrus bretschneideri[J].Acta Horticulturae Sinica(园艺学报),1996,23(1):23￣26.(in Chinese)
• [17]RAO M V,PALIYATH G,ARMROD D P.Ultraviolet-b radiation and ozone-induced biochemical changes in the antioxidant enzymes ofArabidopsis thaliana[J].Plant Physiol,1996,110:125￣136.
• [18]QIE GF,LIUJ H,DONGXY,et al(郄光发,刘俊华,董晓颖,等).Relationship between coloration and antioxidant enzymes in Fuji apple fruit[J].Acta Horticulturae Sinica(园艺学报),2004,31(3):347～349.(in Chinese)
• [19]NISHIHARAE,KONDOK,PARVEZMM,etal.Roleof5-aminole-vulinic acid(ALA)on active oxygen-scavenging system in NaCl-treated spinach(Spinacia oleracea)[J].J Plant Physiol,2003,160:1085￣1091.
• [20]HIRASUNA T J,SHULER M L,LACKNEYV K,et al.Enhanced anthocyanin production in grape cell cultures[J].Plant Sci,1991,78(1):107￣120.
• [21]GOLLOP R,FARHI S,PERL A.Regulation of the leucoanthocyani-din dioxygenase gene expression in Vitis vinifera[J].Plant Sci,2001,161:579￣588.
• [22]WANG L J,SHI W,HOU X L,et al.(汪良驹,石伟,侯喜林,等)Effects of exogenous5-aminolevulinic acid treatment on leaf pho-tosynthesis of pak-choi[J].J Nanjing Agri Univer(南京农业大学学报),2004(2):34￣38.(in Chinese)