杧果蒂腐病菌对多菌灵的抗药性测定及其杀菌剂筛选Resistance of Botryodiplodia theobromae to carbendazim and the fungicides screening using stem end rot of mango fruit as a control
胡美姣;师超;安勇;李敏;杨凤珍;高兆银;
摘要(Abstract):
对来自海南3个地区的86个杧果蒂腐病菌(Botryodiplodia theobromae Pat.)菌株进行了对多菌灵的抗药性测定,结果表明,杧果蒂腐病菌群体中存在着对多菌灵抗性的群体。采用生长速率法进行了23种杀菌剂对4株杧果蒂腐病菌的室内毒力测定,通过EC50值、EC90值及与多菌灵的交互抗性等的综合分析,结果表明,作为防治杧果蒂腐病的首选药剂有咪鲜胺锰盐、丙环唑、氟硅唑、咪鲜胺、异菌脲、苯醚甲环唑、戊唑醇、腈菌唑、吡唑醚菌酯和井冈霉素10种杀菌剂,可选杀菌剂还有百菌清和代森锰锌。杧果蒂腐病菌除对多菌灵产生抗性外,还对甲基硫菌灵、醚菌酯和烯唑醇3种杀菌剂产生了抗药性。通过交互抗性分析,多菌灵、甲基硫菌灵和烯唑醇3种杀菌剂之间存在交互抗性,而醚菌酯与井冈霉素之间存在负交互抗性。为了避免杧果蒂腐病菌对某种杀菌剂产生抗药性,建议上述可选用杀菌剂交替使用。
关键词(KeyWords): 杧果;蒂腐病菌;杀菌剂;抗药性;筛选
基金项目(Foundation): 国家科技支撑计划(2007BAD48B05);; 公益性行业科研专项(nyhyzx07-032-2);; 中央级公益性科研院所基本科研业务费专项(N0.2007hzs1J006、N0.2008hzs1J001、N0.2008hzs1J012);; 热带农业科学院基金(Rky0708)
作者(Author): 胡美姣;师超;安勇;李敏;杨凤珍;高兆银;
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DOI:
参考文献(References):
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