‘次郎’柿成熟叶多酚类物质分布和积累的研究Distribution patterns of polyphenolic compounds in leaves of Diospyros kaki 'Cilang'
刘林
摘要(Abstract):
【目的】为了解多酚类物质在‘次郎’柿成熟叶组织和细胞中的分布与积累特点,【方法】根据多酚类物质与锇作用形成稳定螯合物的特性,用四氧化锇处理样品,在光学和电子显微镜下观察。【结果】结果表明,多酚类物质分布于叶片各种组织中,但分布不均匀,上表皮多数细胞充满多酚类物质,下表皮少数细胞含多酚类物质,且含量少;栅栏组织多数细胞含多酚类物质,海绵组织少数细胞含多酚类物质,且含量少;韧皮部和同侧维管束鞘有较多含多酚类物质的细胞,而木质部和同侧维管束鞘基本没有含多酚类物质的细胞;多酚类物质沿液泡膜内表面均匀沉积,在液泡周缘呈一层,或充满液泡。【结论】显然,多酚类物质在"次郞"柿成熟叶中的分布有位置特异性,负责前体转运和加工的装置在液泡膜上密集而均匀地分布。
关键词(KeyWords): 柿;叶;多酚类物质;超微结构
基金项目(Foundation): 临沂大学科研基金(HX09104);; 临沂市科技局计划项目(200101);; 山东省教育厅科研计划项目(J01H51)
作者(Author): 刘林
DOI: 10.13925/j.cnki.gsxb.2012.04.024
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