胡玉林;谢江辉;江新华;左雪冬;周红霞;

• 1:中国热带农业科学院南亚热带作物研究所

摘要(Abstract)：

为了比较威廉斯香蕉8818及其抗枯萎病突变体8818-1在细胞学和组织解剖学上存在的差异,对这2种香蕉的体细胞染色体数目、气孔保卫细胞的形态特征和根系细胞结构等进行了相关研究。结果表明,8818和8818-1染色体数目均分布在28～37,属三倍体,其整倍体染色体数目为2n=3x=33,8818和8818-1体细胞中的整倍体个数分别占观察总数的41%和52%,除整倍体外,2者体细胞中还存在大量的非整倍体;8818-1的气孔保卫细胞长度与宽度,气孔密度均比8818大,而2者保卫细胞内叶绿体数目差异不明显;8818-1的根系中导管直径和两穿空板间的距离均显著比8818小,而单位面积上的导管数和薄壁细胞数目比8818多。

关键词(KeyWords)： 威廉斯香蕉;抗病突变体;细胞学;组织学

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务专项(NYS200703);; 国家科技基础条件平台重点项目(2005DKA21000-5-05);; 海南省自然基金项目(807039)

作者(Author): 胡玉林;谢江辉;江新华;左雪冬;周红霞;

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DOI:

参考文献(References)：

• [1]HWANG S C.Proceedings of the international workshop on the ba-nana fusarium wilt diseasc[C].Los Banos,2001:39-49.
• [2]LIU Hai-rui.Sereening the resistant mutants to banana vaseular wilt using the erude toxins of Fusarium oxyporum f.sp.eubense[D].Fu-jian Agrieulture and Forestry University,2007.刘海瑞.应用病菌毒素筛选香蕉抗枯萎病突变体[D].福建农林大学,2007.
• [3]XU Xue-lian,DAI Peng.Isolation and identification of two entophyt-ic bacter ia str ains against Fusarium oxysporum[J].Journal of Fruit Science,2007,24(4):483-486.徐雪莲,代鹏.2株抗枯萎病尖镰孢菌内生细菌菌株的分离及鉴定[J].果树学报,2007,24(4):483-486.
• [4]CHEN Ya-ping,CHEN Yun-feng,HUANG Xia,XIAO Wang,HUANG Xue-Lin.PCR-RFLP analysis of three wild bananas and their resistance to fusarium wilt[J].Acta Horticulturae Sinica,2008,35(1):19-26.陈雅平,陈云凤,黄霞,肖望,黄学林.3个野生香蕉株系的PCR-RFLP分析及其对枯萎病抗性的研究[J].园艺学报,2008,35(1):19-26.
• [5]BHAGWAT B,DUNCAN E J.Mutation breeding of banana cv.High-gate(Musa spp.AAA Group)for tolerance to Fusarium oxysporum f.sp.Cubense usingchemicalmutagens[J].ScientiaHorticulturae,1998,73:11-22.
• [6]BHAGWAT B,DUNCAN E J.Mutation breeding of banana cv.High-gate(Musa acu minata,AAA)for tolerance to Fusarium oxysporum f.sp.Cubense using gamma irradiation[J].Euphytica,1998,101:143-150.
• [7]HU Yu-lin,XIE Jiang-hui,LIANG Guo-lu,CHEN Jia-ying,WANG Zhong-meng.Induction and selection of banana mutants r esistant to fusarium wilt disease[J].Journal of Fruit Science,2008,23(2):462-464.胡玉林,谢江辉,梁国鲁,陈佳瑛,王忠猛.香蕉抗枯萎病突变体的诱发与筛选[J].果树学报,2008,23(2):462-464.
• [8]WANG Zheng-xun,LIU Hong-xian.Chromosome aberration in ba-nana micropropagation[J].Journal of Descendiblity,1997,24(6):550-560.王正询,刘鸿先.香蕉苗试管繁殖染色体数量畸变的研究[J].遗传学报,1997,24(6):550-560.
• [9]JIANG Yu-rong,FANG Wei-ping,ZHU Shui-jin,JI Dao-pan.Relationship of verticillium wilt resistance with plant anatomicalstructure and Biochemical metabolism in upland cotton[J].Acta A-gronomica Sinica,2005,31(3):337-341.蒋玉蓉,房卫平,祝水金,季道藩.陆地棉植株组织结构和生化代谢与黄萎病抗性的关系[J].作物学报,2005,31(3):337-341.
• [10]FENG Jie.The present situation and prospect in the study of the in-teraction mechanism between cotton and Verticillium dahliae[J].Ac-ta Gossypii Sinica,1998,10(6):281-284.冯洁.棉花与黄萎病菌互作机制研究现状及展望[J].棉花学报,1998,10(6):281-284.
• [11]BECKMAN M H.Physical barriers associated with resistance in Fusarium wilt of bananas[J].Phytopathology,1961,51:507-515.
• [12]WANG Li.Studies on the mechanism of resistance to Verticillium dahliae in Cotton[D].Taiyuan:Shanxi Agricultural University,2005.王莉.不同抗性的棉花品种抗黄萎病机理的初步研究[D].太原:山西农业大学,2005.