徐国前;栾丽英;焦旭亮;张振文;

• 1:西北农林科技大学葡萄酒学院
• 2:中法合营王朝葡萄酿酒有限公司
• 3:陕西省葡萄与葡萄酒工程技术研究中心

摘要(Abstract)：

基于不同质量浓度Na2CO3溶液、反应温度和时间对反应体系吸光度影响单因素试验基础上,运用二次回归正交旋转组合设计方法优化反应条件;以微量移液器为工具,建立一种简化、微量测定葡萄与葡萄酒总酚含量的方法。结果表明,建立的回归模型达显著水平,利用优化函数途径进行模拟选优得到最佳反应条件为:0.5 mL样品,0.5mL 1 mol·L-1 Folin-Ciocalteu试剂(F-C试剂)和4.0 mL 52 g·L-1 Na2CO3溶液,在25℃下置102 min后,测765 nm吸光度。相对没食子酸质量浓度在0～0.050 g·L-1内,吸光度和没食子酸浓度有良好的线性关系(R2=0.999 4)。对酿酒葡萄赤霞珠与王朝干红葡萄酒样的加样回收率分别是98.4%和97.8%(n=3),相对标准差(RSD)分别为1.32%和2.01%。该法是一种简化、微量经济测定葡萄与葡萄酒总酚含量的方法,且具有较高的稳定性和准确度。

关键词(KeyWords)： 葡萄;葡萄酒;总酚;Folin-Ciocalteu法;二次回归正交旋转组合设计

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项资金(nycytx-30-zp-04)

作者(Author): 徐国前;栾丽英;焦旭亮;张振文;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.03.030

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