毛樱桃嫁接‘美早’甜樱桃小脚现象的导管分子特性研究Vessel element characteristics of scion overgrowing of the sweet cherry variety ‘Tieton’ grafted on Cerasus tomentosa
郭学民;李娜;刘建珍;肖啸;张立彬;
摘要(Abstract):
【目的】砧木直径细于接穗的小脚现象是樱桃栽培中的常见现象。探究毛樱桃(Cerasus tomentosa)作砧木嫁接‘美早’甜樱桃(C.avium‘Tieton’)不同部位次生木质部导管分子特性的差异,以期为揭示小脚现象的结构机制和深入研究该现象提供一定的理论依据。【方法】采用组织离析法、显微照相技术、生物计量统计法与混合取样法,以嫁接5 a的3株‘美早’甜樱桃树为试材,比较了砧木根段、砧木茎段和接穗茎段木质部导管分子的形态和大小。【结果】‘美早’甜樱桃3个部位次生木质部导管分子均为孔纹导管,单穿孔,互列纹孔式,具有长尾导管分子、短尾导管分子和过渡阶段的导管分子,两端具尾、一端具尾和两端无尾的导管分子,端壁倾斜的、中间过渡类型的和两端近水平的导管分子;与其砧木根段和接穗茎段相比较,砧木茎段两端具尾的导管分子数目多5.3%和29.8%、两端无尾的少50.0%和53.8%、两端倾斜的少3.8%和8.3%、端壁倾角小的多22.2%和66.7%、端壁倾角小9.4%和9.5%、端壁倾角小于32°的多75.0%和95.2%、端壁倾角大于52°的少33.3%和16.7%;砧木茎段导管分子长度短29.9%和21.3%,长度大于260μm的均少100%;砧木茎段导管分子宽度大19.5%和22.4%,宽度大于50μm多60.0%和80.0%,即与其他实生树相比较,‘美早’甜樱桃从砧木根系、砧木茎段到接穗茎段导管宽度不匹配。【结论】‘美早’甜樱桃砧木茎段木质部导管分子形态和大小与砧木根段、接穗茎段的差异可能是导致"小脚"现象的重要原因。
关键词(KeyWords): 甜樱桃;毛樱桃;小脚现象;导管分子
基金项目(Foundation): 河北省自然科学基金(C2014407077)
作者(Author): 郭学民;李娜;刘建珍;肖啸;张立彬;
Email:
DOI: 10.13925/j.cnki.gsxb.20160240
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