秦旭;高文胜;周文杰;吕德国;杨力;

• 1:山东农业工程学院
• 2:山东省果茶技术指导站
• 3:沈阳农业大学园艺学院
• 4:山东省农业科学院农业资源与环境研究所

摘要(Abstract)：

【目的】探明控释肥对果园土壤微生物功能的影响,为控释肥在果树上的应用提供理论依据。【方法】以两种养分含量不同的果树专用控释肥CRF1(20-10-15)、CRF3(19-7-19)为试材,设置不同控释肥、减氮控释肥(CRF2)、施用复合肥、不施肥等试验处理,综合利用氯仿熏蒸法和BIOLOG-ECO技术,检测土壤中微生物群落功能多样性的变化。【结果】控释肥对微生物利用碳源能力的影响与果树生育时期有关,果树专用控释肥CRF1与CRF2于果树生长的前期5月28日促进了微生物对碳源的利用,而果树专用控释肥CRF3于8月27日促进微生物对碳源的利用。微生物对不同种类碳源的利用能力随控释肥种类及果树生长时期的不同而有差异,CRF1、CRF2及CRF3处理在果树生长中后期利用最多的碳源依次为聚合物类、氨基酸类及胺类。【结论】与施用普通复合肥相比,施用控释肥可显著增加果园土壤微生物利用碳源的能力,碳水化合物及氨基酸类碳源的利用情况是区分控释肥处理对土壤微生物功能多样性影响的主要碳源。

关键词(KeyWords)： 苹果园;控释肥;土壤微生物;微生物功能多样性

Abstract:

Keywords:

基金项目(Foundation): 十二五国家科技支撑计划项目(2011BAD11B01,2011BAD11B02);; 山东省科技发展计划项目(2012GNC11319);; 山东省农业科技成果转化资金项目(2012065)

作者(Author): 秦旭;高文胜;周文杰;吕德国;杨力;

Email:

DOI: 10.13925/j.cnki.gsxb.20140082

参考文献(References)：

• [1]YAN Xiang,JIN Ji-yun,HE Ping,LIANG Ming-zao.Recent ad-vances in technology of increasing fertilizer use efficiency[J].Scientia Agricultura Sinica,2008,41(2):450-459.闫湘,金继运,何萍,梁鸣早.提高肥料利用率技术研究进展[J].中国农业科学,2008,41(2):450-459.
• [2]GAO Wen-sheng,LüDe-guo.Apple bagging cultivation base[M].Beijing:Chinese Agricultural Science and Technology Press,2010:7-9.高文胜,吕德国.苹果有袋栽培基础[M].北京:中国农业出版,2010:7-9.
• [3]YANG Hong-qiang.Green pollution-free fruit production series[M].Beijing:Chinese Agricultural Science and Technology Press,2003:147.杨洪强.绿色无公害果品生产全编[M].北京:中国农业出版社,2003:147.
• [4]ZHANG Min,SHI Yan-xi,YANG Shou-xiang,YANG Yue-chao.Status quo of study of controlled-release and slow-release fertilizers and progress made in this respect[J].Journal of the Chemical Fertilizer Industry,2001,28(5):27-30.张民,史衍玺,杨守祥,杨越超.控释和缓释肥的研究现状和进展[J].化肥工业,2001,28(5):27-30.
• [5]ZHANG Min,YANG Yue-chao,SONG Fu-peng,SHI Yan-xi.Study and industrialized development of coated controlled release fertilizers[J].Journal of the Chemical Fertilizer Industry,2005,32(5):24-28.张民,杨越超,宋付朋,史衍玺.包膜控释肥料研究与产业化开发[J].化肥工业,2005,32(5):24-28.
• [6]ZHANG Fu-suo,FAN Xiao-lin,LI Xiao-lin.The new trends of research on Soil and plant nutrition[M].Beijing:Chinese Agricultural Science and Technology Press,1995:87-89.张福锁,樊小林,李晓林.土壤与植物营养研究新动态[M].北京:中国农业出版社,1995:87-89.
• [7]SUN Jian,ZHANG Qiang,LIU Song-zhong,WANG Xiao-wei,LIU Jun,WEI Qin-ping.Comparison of soilmicrobe community structure between organic and conventional apple or chards[J].Journal of Fruit Science,2013,30(2):230-234.孙健,张强,刘松忠,王小伟,刘军,魏钦平.有机和常规苹果园的土壤微生物结构差异研究[J].果树学报,2013,30(2)230-234.
• [8]VANCE E D,BROOKES P C,JENKINSON D S.An extraction method for measuring microbial biomass C[J].Soil Biology and Biochemistry,1987,19(6):703-707.
• [9]ISLAM M R,CHAUHAN P S,KIM Y,KIM M,SA T.Community level functional diversity and enzyme activities in paddy soils under different long-term fertilizer management practices[J].Biology and Fertility of Soils,2011,47(5):599-604.
• [10]BORRERO C,ORDOVA’S J,TRILLAS M I,AVILéS M.Tomato Fusarium wilt suppressiveness:The relationship between the organic plant growth media and their microbial communities as characterised by biolog[J].Soil Biology and Biochemistry,2006,38(7):1631-1637.
• [11]GARLAND J L.Analysis and interpretation of community-level physiological profiles in microbial ecology[J].FEMS Microbiology Ecology,1997,24(4):289-300.
• [12]CHEN Wei,JIANG Zhong-wu,HU Yan-li,SHU Huai-rui.Study on biological character of rhizosphere microorganism in apple orchard[J].Journal of Soil and Water Conservation,2008,22(3):168-171.陈伟,姜中武,胡艳丽,束怀瑞.苹果园土壤微生物生态特征研究[J].水土保持学报,2008,22(3):168-171.
• [13]YAN Dong-yun,ZHANG Min,JIANG Xin.Effects of control-re-lease Fertilizers on growth and development of potted Salvia[J].Acta Horticulture Sinica,2004,31(6):773-777.颜冬云,张民,蒋新.控释复合肥对盆栽一串红生长发育与品质的影响[J].园艺学报,2004,31(6):773-777.
• [14]QIU Xian-kui,DONG Yuan-jie,WAN Yong-shan,HU Guo-qing,WANG Yan-hua.Effects of different fertilizing treatment son contents of soil nutrients and soil enzyme activity[J].Soils,2010,42(2):249-255.邱现奎,董元杰,万勇善,胡国庆,王艳华.不同施肥处理对土壤养分含量及土壤酶活性的影响[J].土壤,2010,42(2):249-255.
• [15]LUO Lan-fang,ZHENG Sheng-xian,LIAO Yu-lin,XIE Jian,XIANG Yan-wen,NIE Jun.Effect of controlled release nitrogenfertilizer on soil microbe as well as its relation to soil nitrogen fertilizy[J].Journal of Hunan Agricultural University,2007,33(5):608-613.罗兰芳,郑圣先,廖育林,谢坚,向艳文,聂军.控释氮肥对稻田土壤微生物的影响及其与土壤氮素肥力的关系[J].湖南农业大学学报,2007,33(5):608-613.
• [16]HUANG Hui,CHEN Guang-shui,XIE Jin-sheng,HUANG Chao-fa.Advances on soil microbial biomass carbon and its effect factor[J].Hubei Forestry Science and Technology,2008(4):34-41.黄辉,陈光水,谢锦升,黄朝法.土壤微生物生物量碳及其影响因子研究进展[J].湖北林业科技,2008(4):34-41.
• [17]XU Hua-qin,XIAO Run-lin,ZOU Dong-sheng,SONG Tong-qing,LUO Wen,LI Sheng-hua.Effects of long-term fertilizationon functional diversity of soil microbial community of the tea plantation[J].Acta Ecologica Sinica,2007,27(8):3356-3361.徐华勤,肖润林,邹冬生,宋同清,罗文,李盛华.长期施肥对茶园土壤微生物群落功能多样性的影响[J].生态学报,2007,27(8):3356-3361.
• [18]ZHANG Yi-fei,ZONG Wen-hui,LI Zhong-pei,CAI Zu-cong.Effects of Long-Term different fertilization on soil enzyme activity and microbial community functional diversity panddy soil derived from quaternary red clay[J].Journal of Ecology and Rural Environment,2006,22(4):39-44.张逸飞,钟文辉,李忠佩,蔡祖聪.长期不同施肥处理对红壤水稻土酶活性及微生物群落功能多样性的影响[J].生态与农村环境学报,2006,22(4):39-44.
• [19]SUN Feng-xia,ZHANG Wei-hua,XU Ming,ZHANG Wen-ju,LI Zhao-qiang,ZHANG Jing-ye.Effects of long-term fertilization on microbial biomass carbon and nitrogen and on carbonsource utilization of microbes in a red soil[J].Chinese Journal of Applied Ecology,2010,21(11):2792-2798.孙凤霞,张伟华,徐明,张文菊,李兆强,张敬业.长期施肥对红壤微生物生物量碳氮和微生物碳源利用的影响[J].应用生态学报,2010,21(11):2792-2798.
• [20]SHEN Wei-shou,LIN Xian-gui,ZHANG Hua-yong,YIN Rui,DUAN Zeng-qiang,SHI Wei-ming.Microbial activity and func-tional diversity in soils used for the commercial production of cu-cumbers and tomatoes in polytunnel greenhouse,under different fertilization[J].Acta Ecologica Sinica,2008,28(6):2683-2688.申卫收,林先贵,张华勇,尹睿,段增强,施卫明.不同施肥处理下蔬菜塑料大棚土壤微生物活性及功能多样性[J].生态学报,2008,28(6):2683-2688.
• [21]XIA Xue,GU Jie,CHE Sheng-guo,GAO Hua,QIN Qing-jun.Effects of nitrogen application rates on microbial community and enzyme activities in Lou Soil[J].Scientia Agricultura Sinica,2011,44(8):1618-1627.夏雪,谷洁,车升国,高华,秦清军.施氮水平对塿土微生物群落和酶活性的影响[J].中国农业科学,2011,44(8):1618-1627.