胡钟东;乔玉山;陶建敏;渠慎春;章镇;

• 1:南京农业大学园艺学院
• 2:南京农业大学园艺学院
• 3:南京农业大学园艺学院
• 4:南京农业大学园艺学院

摘要(Abstract)：

根据已报道的砂梨ACO3(GenBank Accession No.AB042107)序列设计并合成一对特异引物,以砂梨品种若光(Wakahikari)叶片总DNA为模板,利用染色体步移技术扩增基因的上游调控序列,回收该片段并克隆到pMD19-T载体,测序后去除接头和基因编码序列。得到1段长715bp的上游序列,登陆PLACE数据库在线进行植物DNA顺式作用元件预测并分析,起始位点的上游89bp处有1个TATA-Box核心启动子元件。另外序列还存在多个特异调控基因表达的元件,初步判断为砂梨果实中ACC氧化酶基因的诱导型启动子。

关键词(KeyWords)： 砂梨;ACC氧化酶;调控序列;序列分析

Abstract:

Keywords:

基金项目(Foundation): 江苏省高技术研究(BG2006313);; 南京农业大学青年基金(KJ05007)

作者(Author): 胡钟东;乔玉山;陶建敏;渠慎春;章镇;

Email:

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