赞皇大枣的群体遗传多样性评价Genetic diversity evaluation of Zizyphus jujuba cv. Zanhuangdazao
刘平;彭建营;彭士琪;周俊义;
摘要(Abstract)：
为了探明三倍体赞皇大枣的遗传基础,利用22个多态性引物对赞皇县及周边县区的50个赞皇大枣类型、26个酸枣类型、8个枣品种等3个群体进行了RAPD扩增,共扩增285个位点。遗传多样性分析表明:赞皇大枣群体的多态性位点百分率为58％,小于枣品种群体(66％)和酸枣群体(89％);赞皇大枣群体共扩增出205种带型,而酸枣群体扩增出416种带型;赞皇大枣群体内平均遗传距离为0．087,小于枣品种群体(0．181)和酸枣群体(0．254)。说明:赞皇大枣的遗传多样性小于枣品种群体,酸枣群体有较丰富的遗传背景。
关键词(KeyWords)： 枣;赞皇大枣;酸枣;RAPD;群体;遗传多样性
基金项目(Foundation): 河北省自然科学基金资助项目(300123)。
作者(Author): 刘平;彭建营;彭士琪;周俊义;
Email:
DOI: 10.13925/j.cnki.gsxb.2006.05.006
参考文献(References)：
- [1] PENGJY, LIUP, ZHOUJY, et al(彭建营,刘平,周俊义,等). Karyotypes of different strains in Ziziphus jujuba Mill. Zanhuang-dazao[J]. Acta Horticulturae Sinica(园艺学报), 2005,32 (5): 798-801. (in Chinese)
- [2] LIU X J, ZHENG Y L, LIU J L(刘勋甲,郑用琏,刘纪麟). Genetic diversity evaluation of maize recurrent selection population With RAPD marker[J]. Scientia Agrieultura Sinica(中国农业科学), 1999,32(3): 11-20. (in Chinese)
- [3] WUYM, PE I D, XI S K, et al(吴燕民,裴东,奚东珂,等). Study on relationships between species of Juglans by using RAPD tech-niques[J]. Acta Horticulturae Sinica(园艺学报),2000,27(1):17-22. (in Chinese)
- [4] HARADA T. DNA-RAPDs detect genetic variation and paternity in Malus[J]. Euphytica, 1993,65(2):87-91.
- [5] Francois L. DNA amplified fingerprinting, a useful tool for determination of genetic orign and diversity analysis in Citrus [J]. HortSci-ence, 1995,30(5): 1063-1067.
- [6] LIU P (刘平). Molecular evaluation of genetic variation in Ziziphus jujuba Mill. cv. Zanhuangdazao and its related populations (赞皇大枣及相关类群群体变异的分子评价)[D]. Baoding: Agricultural University of Hebei, 2002. (in Chinese)
- [7] SKROCH P, TIVANG J, NIENHAUS J. Analysis of genetic rela- tionships using RAPD marker data[J]. J Plant Breed Symp Applications of RAPD Technology to Plant Breeding Minneapolis, 1992, 26.
- [8] GE S, HONG D Y(葛颂,洪德元). Biosystemnatic studies on Adenophora potaninii Korsh. complex (Campanulaceae) IV. allozyme variation and differentiation[J]. Acta Phytotaxonomica Sinica(植物分类学报), 1998,36(6):481 -489. (in Chinese)
- [9] GITZENDANNER M A,SOLTIS P S. Patterns of genetic variation in rare and widespread plant congeners[J]. American Journal of Botany, 2000,87(6):783-792.
- [10] PAPPERT R,HAMRICK J L, DONOVAN L A. Genetic variation in Pueraria lobata(Fabaceae), an inteoduced , clonal, invasive plant of the southeastern United States[J]. American Journal of Botany, 2000, 87(9):1240-1245.
- [11] VZSSEUR L,AARSSEN L W,BENNETT T. Allozymic variation in local apomictic popylations of lemna minoe (Lwmnaceae) [J]. American Journal of Botany, 1993,80(8):974-979.
- [12] AYRES D R, RYAN F J. Genetic diversity and structure of the narrow endemic Wyethia reticulata and its congener W.bolanderi(Aster-oceae)using RAPD and allozyme techniques[J]. American Journal of Botany, 1999,86(3):344-353.
- [13] BELAJ A, RALLO L, TRUJILLO I. Using RAPD and AFLP markers to distinguish individuals obtained by clonal selection of Arbe-quina and Manzanilla desevilla olive[J] . HortScience, 2004,39(7): 1566-1570.