张鹏;秦嗣军;周文杰;吕德国;

• 1:沈阳农业大学园艺学院·沈阳市北方果树栽培与生理生态重点实验室

摘要(Abstract)：

【目的】探讨根际优势细菌对东北山樱幼苗根系功能的影响机制,为樱桃园根际环境调控提供参考依据。【方法】以东北山樱幼苗为试材,进行土壤接种荧光假单胞菌(Pseudomonas fluorescens)处理,通过测定土壤微生物生物量碳、根系呼吸速率、幼苗生长量等指标,分析幼苗对接种根际优势细菌的响应特征。【结果】与对照相比,接种荧光假单胞菌的处理土壤微生物生物量碳短期内提高了22.7%、幼苗根系总呼吸速率增加了39.5%、呼吸途径前期以糖酵解途径—磷酸戊糖途径—细胞色素途径(EMP-PPP-COX)为主,而后期转变为以糖酵解途径—三羧酸循环—细胞色素途径(EMP-TCA-COX)为主。不同时间呼吸代谢关键酶活性不同,接种处理前期显著降低了磷酸果糖激酶(PFK)、苹果酸脱氢酶(MDH)活性,而后期显著增加了苹果酸脱氢酶(MDH)活性。接种处理对幼苗生长量无明显影响。【结论】接种荧光假单胞菌对土壤微生物生物量碳的影响存在短期效应。幼苗根系呼吸代谢过程对接种处理的响应有一个滞后期,即一般在处理结束20 d后,根系呼吸途径分配比例,部分相关酶活性发生改变,影响根系总呼吸速率,但最终对幼苗生长量无明显影响。

关键词(KeyWords)： 东北山樱;呼吸代谢;荧光假单胞菌

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30871688、30900967);; 高等学校博士学科点博导类科研基金(20122103110012)

作者(Author): 张鹏;秦嗣军;周文杰;吕德国;

Email:

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