刘巧;张剑侠;于冬冬;王跃进;

• 1:西北农林科技大学园艺学院·旱区作物逆境生物学重点实验室·农业部西北园艺植物种质资源利用重点开放实验室

摘要(Abstract)：

【目的】探讨提高抗寒无核葡萄胚挽救育种效率的方法。【方法】以4个种子败育型无核葡萄品种‘火焰无核’‘海王星’‘木星’和‘红宝石无核’为母本,欧山杂种(品种)‘北醇’为父本进行杂交,将杂交胚珠接种于添加不同质量浓度6-BA的MM3+500 mg·L-1水解酪蛋白(CH)+1 mmol·L-1丝氨酸(Ser)培养基上进行培养。【结果】除‘海王星’ב北醇’外,供试的其他杂交组合均在添加6-BA质量浓度为0.5 mg·L-1时,胚珠发育率、萌发率和成苗率最高,差异达到显著水平;当6-BA的质量浓度为1.0 mg·L-1时,对胚挽救效率具一定程度的促进作用或作用不显著;当6-BA的质量浓度为1.5 mg·L-1时,对‘火焰无核’ב北醇’‘木星’ב北醇’‘海王星’ב北醇’杂交后代胚的成苗起到一定程度的抑制作用。【结论】不同的杂交组合对不同质量浓度6-BA的敏感程度不同,0.5 mg·L-16-BA能够提高无核葡萄胚挽救效率;首次选用的抗寒无核品种‘木星’和‘海王星’均可作为无核葡萄胚挽救育种的母本。

关键词(KeyWords)： 无核葡萄;杂交;胚挽救;6-BA

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系建设专项基金资助项目(CARS-30-yz-7);; 西北农林科技大学唐仲英作物育种基金(2014-97)

作者(Authors): 刘巧;张剑侠;于冬冬;王跃进;

DOI: 10.13925/j.cnki.gsxb.20150048

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