李雄伟;孟宪桥;贾惠娟;王力荣;王志强;马瑞娟;吴大军;董冰;Maria José Aranzana;Pere Arús;高中山;

• 1:浙江大学果树科学研究所·农业部园艺植物生长发育与生物技术重点开放实验室
• 2:中国农业科学院郑州果树研究所
• 3:江苏省农业科学院园艺研究所
• 4:浙江奉化水蜜桃研究所

摘要(Abstract)：

【目的】选择通用的荧光SSR分子标记建立桃种质资源基因型数据库便于进行种质特异性鉴定、资源交换、管理和利用。【方法】根据48个SSR标记对国内外669份桃品系的遗传多样性测试结果,从中选择出一部分多态信息含量较高、适合品种鉴别的标记,采用不同荧光标识组合在ABI 3130测序仪测试,为了使不同批次的测试结果准确可靠,6~8个参照品种纳入每次的测试中。【结果】用16个SSR标记基本可以判断品种的特异性,它们可以用4种荧光标识进行标记,便于分为4组在ABI遗传分析仪上测试。目前数据库包含641份材料,基因型一致的品系多为同一品种突变体和自交系演化的地方品种。文中列出了100份中国代表性种质资源分子标记数据库,并建议8个中国品系为参照。对17个桃品种进行系谱验证发现,我国资源圃所育成的7个品种系谱信息正确,但日本桃品种系谱信息与文献出现偏差。【结论】应用16个多态性高的SSR荧光标记可以构建精确的桃品种分子指纹数据库,测试方法及结果对品种特异性和系谱鉴定提供重要的参考,推动资源分子指纹数据库标准化建设工作。

关键词(KeyWords)： 桃;荧光标记SSR;指纹图谱数据库;品种鉴定;系谱鉴定

Abstract:

Keywords:

基金项目(Foundation): 科技部中国-欧盟科技合作项目(1114);; 公益性行业(农业)科研专项果树遗传改良与控制技术研究及其应用(200903044);; 浙江省高校重大科技攻关项目(ZD2009007);; 浙江省农业新品种选育重大科技专项(2012C12904)

作者(Author): 李雄伟;孟宪桥;贾惠娟;王力荣;王志强;马瑞娟;吴大军;董冰;Maria José Aranzana;Pere Arús;高中山;

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DOI:

参考文献(References)：

• [1]WANG Zu-hua,ZHUANG En-ji.Encyclopedia of Fruit in ChinaPeach[M].Beijing:China Forestry Press,2001.汪祖华,庄恩及.中国果树志—桃卷[M].北京:中国林业出版社,2001.
• [2]WANG Li-rong,ZHU Geng-rui,FANG Wei-chao.Peach genetic resource in China[M].Beijing:China Agricultuve Press,2012.王力荣,朱更瑞,方伟超.中国桃遗传资源[M].北京:中国农业出版社,2012.
• [3]RAJWANT K K,MANOJ K R,SANJAY K,ROHTAS S,DHAWAN A K.Microsatellite markers:an overview of the recent progress in plants[J].Euphytica,2011,177(3):309-334.
• [4]CHENG Z,HUANG H:SSR fingerprinting Chinese peach cultivars and landraces(Prunus persica)and analysis of their genetic relationships[J].Scientia Horticulture,2009,120(2):188-193.
• [5]ARANZANA M J,CARBO J,AR?S P.Microsatellite Variability in Peach[Prunus Persica(L.)Batsch]:Cultivar Identification,Marker Mutation,Pedigree Inferences and Population Structure[J].Theoretical and Applied Genetics,2003,106:1341-1352.
• [6]BOUHADIDA M,MARíAíM,MARA J G,JOSíMA,YOLANDA G.Genetic variability of introduced and local spanish peach cul-tivars determined by SSR markers[J].Tree Genetics&Genomes,2011,7(2):257-270.
• [7]LI X W,MENG X Q,JIA H J,YU M L,MA R J,WANG L R,CAO K,SHEN Z J,NIU L,TIAN J B,CHEN M J,XIE M,ARUS P,GAO Z S,ARANZANA M J.Peach genetic resources:diversity,population structure and linkage disequilibrium[J].BMC Genetics,2013,14:84.
• [8]ARANZANA M J,ABBASSI E K,HOWAD W,ARUS P.Genetic variation,population structure and linkage disequilibrium in peach commercial varieties[J].BMC Genetics,2010,11(1):69.
• [9]ZHANG S M,XU C J,GAO Z S,CHEN K S,WANG G Y.Development and characterization of microsatellite markers for Chinese bayberry(Myrica Rubra Sieb.&Zucc.)[J].Conservation Genetics,2009,10(5):1605-1607.
• [10]LIU K J,MUSE S V.PowerMarker:an integrated analysis environment for genetic marker analysis[J].Bioinformatics,2005,21(9):2128-2129.
• [11]VOORRIPS R E,BINK M C A M,VAN DE WEG W E.Pedimap:software for the visualization of genetic and phenotypic data in pedigrees[J].Journal of Heredity,2012,103(6):903-907.
• [12]CIPRIANI G,LOT G,HUANG W G.MARRAZZO M T,PETERLUNGER E,TESTOLIN R.AC/GT and AG/CT microsatellite repeats in peach[Prunus persica(L.)Batsch]:Isolation;characterisation and cross-species amplification in Prunus[J].Theoretical and Applied Genetics,1999,99(12):65-72.
• [13]ARANZANA M J,GARCIA-MAS J,CARBO J,ARUS P.Development and variability analysis of microsatellite markers in peach[J].Plant Breeding,2002,121(1):87-92.
• [14]DIRLEWANGER E,COSSON P,TAVAUD M,ARANZANA M J,POIZAT C,ZANETTO A,ARUS P,LAIGRET F.Development of microsatellite markers in peach[Prunus persica(L.)Batsch]and their use in genetic diversity analysis in peach and sweet cherry(Prunus avium L.)[J].Theoretical and Applied Genetics,2002,105(1):127-138.
• [15]JONY C,GANDELIN M H,COLLONNIER C,GUITOUNI C,PANHELEUX A L,ZHANG D,FOUILLOUX S,PASCAL T,TUERO C,SZANI Z,ZUBOU L,CHOME P,BADENES M T,SOLER G,MARTINEZ J,ALONSO J M,VERDE I,DETTORI M T,SEMON S.Management of Peach tree reference collections:ongoing research&development program relevant to the community plant variety rights protection system[C].VII International Peach Symposium,2009:51-56.
• [16]LI TH,LI YX,LI ZC,ZHANG HL,QI YW,WANG T.Simple Sequence repeat analysis of genetic diversity in primary core collection of peach(Prunus persica)[J].Journal of Integrative Plant Biology,2008,50(1):102-110.
• [17]XIE R J,LI X W,CHAI M L,SONG L J,JIA H J,WU D J,CHEN M J,CHEN K M,ARANZANA M J,GAO Z S.Evaluation of the genetic diversity of Asian peach accessions using a selected set of SSR markers[J].Scientia Horticulturae,2010,125(4):622-629.
• [18]CHEN Chang-wen,CAO Ke,WANG Li-rong,ZHU Geng-rui,FANG Wei-chao.Molecular ID establishment of main China peach varieties and peach related species[J].Scientia Agricultura Sinica,2011,44(10):2081-2093.陈昌文,曹珂,王力荣,朱更瑞,方伟超.中国桃主要品种资源及其野生近缘种的分子身份证构建[J].中国农业科学,2011,44(10):2081-2093.
• [19]ARANZANA M J,PINEDA A,COSSON P,DIRLEWANGER E,ASCASIBAR J,CIPRIANI G,RYDER C D,TESTOLIN R,ABBOTT A,KING G J,IEZZONI A F,ARUS P.A set of simple-sequence repeat(SSR)markers covering the Prunus genome[J].Theoretical and Applied Genetics,2003,106(5):819-825.
• [20]YAMAMOTO T,MOCHIDA K,HAYASHI T.Shanhai Suimitsuto,one of the origins of Japanese peach cultivars[J].Japanese Society for Horticultural Science,2003,72(2):116-121.
• [21]XU D H,WAHYUNI S,SATO Y,YAMAGUCHI M,TSUNEMATSU M,BAN T.Genetic diversity and relationships of Japanese peach(Prunus persica L.)cultivars revealed by AFLP and pedigree tracing[J].Genetic Resources and Crop Evolution,2006,53(5):883-889.
• [22]EVANS K M,PATOCCHI A,REZZONICO F,MATHIS F,DUREL C E,BOUDICHEVSKAIAA,DUNEMANN F,STANKIEWICZKOSYL M,GIANFRANCESCHI K M,LATEUR M,MADDURI M,NOORDIJK Y,VAN DE WEG W E.Genotyping of pedigreed apple breeding material with a genome-covering set of SSRs:trueness-to-type of cultivars and their parentages[J].Molecular Breeding,2010,28(4):535-547.