赵先炎;赵强;刘鑫;郝玉金;由春香;

• 1:作物生物学国家重点实验室·国家苹果工程技术研究中心·山东农业大学园艺科学与工程学院

摘要(Abstract)：

【目的】将苹果Md DRB1基因转入番茄中,探究苹果MdDRB1基因在番茄中的异位表达与功能。【方法】采用农杆菌介导法,将苹果基因Md DRB1转入番茄植物体内,用PCR检测到该基因已整合到番茄总DNA中。对获得的5个转基因番茄株系进行半定量RT-PCR分析,挑选MdDRB1基因表达水平由低到高的3个株系,用实时荧光定量PCR检测与生长素有关的mi RNAs及相应靶基因的相对表达量。并对非转基因和转基因番茄进行3μmol·L-1IAA和5μmol·L-1IAA处理,对植株进行向光性检测。【结果】转基因株系中miR164、miR160、mi R393表达量下调,mi R167表达量上调,同时,它们分别对应的番茄中的靶基因SlNAC1、SlARF10和SlARF16、SlTIR1的相对表达量上调,SlARF8相对表达量下调。IAA处理后,转基因株系幼苗的侧根数目比未转基因植株增多,同时,转基因株系幼苗的向光性也增强。【结论】苹果Md DRB1基因在番茄中的异位表达,通过抑制生长素响应相关的mi R164、miR160、miR393和增加miR167的积累水平,部分解除其对下游靶基因SlNAC1、SlARF10和Sl ARF16、SlTIR1以及增加Sl ARF8的转录后基因沉默作用,进而提高了转基因番茄对生长素响应的敏感性。

关键词(KeyWords)： 苹果MdDRB1基因;番茄;生长素响应;miRNAs

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(项目号31171946);; 教育部“长江学者和创新团队发展计划”创新团队(IRT1155)

作者(Author): 赵先炎;赵强;刘鑫;郝玉金;由春香;

Email:

DOI: 10.13925/j.cnki.gsxb.20140335

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