王永江;李中安;张振臣;杨方云;周彦;曹孟籍;申晚霞;刘金香;周常勇;

• 1:国家柑桔工程技术研究中心
• 2:河南省农业科学院植物保护研究所

摘要(Abstract)：

【目的】预测鉴定甜橙miRNA及其作用机制和功能,为深入研究挖掘甜橙miRNA及其抗病相关miRNA的作用等提供翔实的基因信息。【方法】利用高通量测序技术、相关的生物信息学软件和数据库,构建甜橙小RNA文库,然后以甜橙基因组为参考基因组,进行甜橙miRNA鉴定和靶基因预测;利用GO和KEGG数据库对靶基因进行功能分析。【结果】成功构建了甜橙小RNA文库:获得12 617 944条小RNA序列分属于3 065 625种小RNA序列,获得序列中24 nt长的序列最多,占到序列的49.3%。鉴定出2 199 561条miRNA,其中已知miRNA638 722条,分属52种miRNA家族,候选miRNA1 560 839条,分属204种miRNA。已知的52种保守miRNA基因预测出927个靶基因位点,候选miRNA中有154种预测出1 777个靶基因位点。GO数据库对靶基因分类显示,甜橙miRNA调控的靶基因主要参与多细胞互作、转录本调控和细胞组成等生物学途经和分子功能方面。KEGG数据库中病原物与寄主互作亚库分析结果显示,靶基因主要与抗性蛋白、转录因子和抗病酶等关联。【结论】预测鉴定出一批甜橙miRNA及其调控的靶基因,为进一步研究提供了参考信息。

关键词(KeyWords)： 甜橙;miRNA;靶基因;高通量测序

Abstract:

Keywords:

基金项目(Foundation): 农业部行业公益项目(200903004-06,201203076-01);; 科技支撑计划(2012BAD19B00);; 教育部创新团队(IRT0976)

作者(Authors): 王永江;李中安;张振臣;杨方云;周彦;曹孟籍;申晚霞;刘金香;周常勇;

DOI: 10.13925/j.cnki.gsxb.2013.04.011

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