砂糖橘上碎叶病毒外壳蛋白基因的克隆及序列分析Cloning and sequencing the coat protein gene of citrus tatter leaf virus from Shatangju mandarin cultivar
孟祥春;向旭;钟云;刘岩;易干军;
摘要(Abstract)：
利用RT-PCR技术对广东砂糖橘碎叶病毒的外壳蛋白(CTLV-CP)基因进行了克隆和测序分析。DNA序列分析表明,砂糖橘CTLV-CP基因的cDNA序列全长为786bp,编码262个氨基酸。BLAST分析结果显示,所得序列与苹果、梨茎沟病毒(ASGV)外壳蛋白基因及其它柑橘CTLV-CP基因的同源性在87%～91%,证明所克隆的片段为CTLV-CP基因。
关键词(KeyWords)： 砂糖橘;碎叶病毒(CTLV);外壳蛋白(CP);克隆
基金项目(Foundation): 广东省科技计划项目(2005B50201002);; 广东省农科院博士启动项目(05-博士-02)
作者(Author): 孟祥春;向旭;钟云;刘岩;易干军;
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